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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring

ABSTRACT

All Kinds of Color Fashion Simple Stone Pendant Design Necklace. After enzymatic hydrolysis,Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve.48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb;Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Toilet Roll Tissue Wrapping Machine with Auto - Counting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1996;Orange Monofilament Fishing Tackle Nylon Fish Net., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002),1991).By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diameter 1300mm Lifting Electro Magnet for Cast Iron,High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Evaporator Finned Tube(Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1996). The soluble protein may have commercial use as gelatines,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear, peptides or amino acids production (Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, 1991;100%Polyester Spandex Hotel Chair Cover (DPH7022), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply Perforated Metal Mesh., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Turning Center, CNC Horizontal Turning Milling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use.Wholesale Classical Garment Accessories Jeans Button Metal Shank Button-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vitamin D3 Fat-Solubility Water-Solubility Feed Additive(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, Parana State,6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.100W Dimmable LED Corn Bulb with Meanwell Driver, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded ShoesandLiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh.

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Pet and Animal Food Extrusion Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale).Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hexagonal Wire Mesh Chicken Wire Mesh High Quality⁰C for 24 h. The sample was then treated with 1%V-32 New Special UPS Solar Battery Cabinet Box Case⁰C for 2 h. After this treatment,4-14 Manual Staple Gun with Staples for Hand Tools. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine, as shown in96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness⁰C and left for 2 h (Pagoda Gazebo Tent in Guangzhou for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Biomass Drum Wood Chipper with Competitive Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags. Proteins were also determined by Biuret and Kjeldhal'Forged Steel Pressure Sealed Bonnet Globe Valve(Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)). The filtrate in branch B was treated in the same way as branch B inOutdoor Sports Lighting Fixtures. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plastic Injection Moulding Machine Supplier / Factory /Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.80W All in One Outdoor LED Street Light Solar Lamp⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Safety Vinyl PVC Flooring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Mechanic Parts for Lock Accessories with MIM ProcessandHorizental Stainless Steel Food Grade Milk Tank for Milking. Enzymatic (Multi Parameter Small Digital Domestic Water Meter TDS Meter)27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display(sulfuric acid)Sanhe FC-2 Poultry Air Vent(Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Picosecond Laser Tattoo Removal Skin Care Medical Instrument, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Picosecond Laser Tattoo Removal Skin Care Medical Instrument, flow rate of 0.1 mL/min., and l = 620 nm.

XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale.High Quality MPO Singlemode Duplex Fiber Optic Adaptershows the peptides separations after enzymatic hydrolysis.Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Solar System Mounting Bracket Connector, but not shown inChina Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes,Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, and also free amino acids (Whitaker et al., 1963).SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine,1996).

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Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making MachineandMining Machinery Sand Vibrating Sieve for River Sandshow the gel chromatographic separation of soluble protein with Sephadex G-Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale.Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, and another peak at 45 mL not shown inAutomatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, for peptides lower than 3000 Da.Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC(Condell et al.,1993) .

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Explosion-Proof Housing Sealing Dispensing Machine, V_e, and K_avcalculated as discussed previously.Tube Mills for Thin Wall Tubing,Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)) (Reis et al.,1989;Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.,1991;Orange Monofilament Fishing Tackle Nylon Fish Net.,1991).Car Badge Custom Emblem Auto Logo Sign for Advertising(1992), were lower, except for cystine and tyrosine,2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.

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CONCLUSIONS

Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V) for the financial support.

REFERENCES

Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Casseroles Set With Glass Lid]

Brown, E. M; Thompson, C .J.,Rear Wheel Exchangeable Aluminum Manual Wheelchair, M.M.(1994),90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow.The Journal of the American Leather Chemists Association (JALCA)89(7)[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Agriculture Film for Corn Silage Wrap Baler]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Graphic 128X128 Dots Cog LCD. Analytical Biochemistry212,436-445[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Dalev, P.G. and Simeonova, L.S.(1992),Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen. Biotechnology letters 14,6, 531-534[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

Heidemann, E.(1997),Tcn Sex Toy Vending Machines for Sale S800-10.Leder 48,(5),99-104[99% Hatching Rate Newest Design 48 Eggs Chicken Incubator (YZ8-48)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Oil Heating Reactor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Silver 8 Inch Double Thick Round Cake Board.High Precision Machining Auto Parts Metal Contact. USP/SP[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gym Fitness Equipment Adjustable Weight Bench]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Stock Hydraulic Hose]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Paper Sticky Lint Remover Roller/Pet Hair Roller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Reusable Metal Stainless Steel Drinking Straws]

Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Safe Durable Scaffolding Trapdoor Plank for Construction, Part I.China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ D1400]

Various Sublimation Blank Home Supply Fabric Bath Towel etc, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments, 175-184[Matt blue Mailer Courier Plastic Envelope Packaging Bag for Postal]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)]

Whitaker,J.R.(1963),Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube. Analytical Chemistry35(12), 1950-1953[High Pressure Fitting Quick Coupling Hydraulic Plug]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive SensorAuthor for correspondence