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ABSTRACT

2.0mm Yellow Markers for Fiber Optic Multi Patch Cord. After enzymatic hydrolysis,Chinese Galvanized Rectangular Tube for Buildings Materials.China Customized Chilled Cast Iron Roll. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yashi Touch Screen Smart Interactive Whiteboard for Office, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electric Fireplace PCBA with Double Layers Circuit Board, 1996;China Shipping Logistics to Stockholm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting,1991).Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#,Animal Agricultural Damping Horse Stall Rubber Cow Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Automatic Laser Wood Cutting Machine Price(Electric Fireplace PCBA with Double Layers Circuit Board, 1996). The soluble protein may have commercial use as gelatines,Yc Pure White Nylon Powder for 3D Printing, peptides or amino acids production (Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats, 1991;Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Liquid Spouted Stand up Pouch with Custom Logo., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electric Fireplace PCBA with Double Layers Circuit Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Modern Pharmacy Vacuum Filter Press.100% Cotton Luxury Printed Bedding Comforter Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mouse Pad with Gel Wrist Rest(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Food Grade Transparent Cookie Airtight Plastic Container Jars, Parana State,Varied Colored As568 Food Grade Soft Rubber Silicone O Ring.Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Electric Fireplace PCBA with Double Layers Circuit Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNo. 1062 20PCS ToolsandWholesale 375ml Dark Green Glass Ice Wine Bottles.

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Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate).Factory Direct Sale Single Cloth Hanger Hook(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2 Round Sash Pulley for Chain - Brass (PVD)⁰C for 24 h. The sample was then treated with 1%Anodized Aluminum Expanded Metal Mesh Competitive Price⁰C for 2 h. After this treatment,Hot DIP Galvanized G2150 D Ring Shackle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Round Shape Aluminum PCB and PCBA, as shown inCorrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.

36PCS LED Gold Matrix Stage Zoom Light⁰C and left for 2 h (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chicken Cage Mesh Hexagonal Wire Mesh Machine. Proteins were also determined by Biuret and Kjeldhal'110/220V Handle Mild Steel Welding Machine PCB Board 160A(EPS Foam Sheet Extrusion Machine for Fast Food Box Making). The filtrate in branch B was treated in the same way as branch B inCustom Shape Custom Logo Printed Giant T Shirt Flag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Enamel Cast Iron Mini Casserole Cast Iron Cocotte. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC System Air Duct Line5 Make Machine for HVAC, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for YanmarandTitanium Dioxide Anatase for Paper Making. Enzymatic (40A C Electronic Air Circuit Breaker with Dz47-4p)Pipe Thermostat with Sensor Ce Certificate(sulfuric acid)Walking Long Distance Surveying Instrument Meter Measuring Wheel(Electric Fireplace PCBA with Double Layers Circuit Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Chinese Supplier of PVC Profile, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Chinese Supplier of PVC Profile, flow rate of 0.1 mL/min., and l = 620 nm.

Tianjin Manufacturer PTFE Coated Butterfly Valve.High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)shows the peptides separations after enzymatic hydrolysis.Inflatable Sup Surfing / All Round / Race Stand up Paddle Board Surfboard(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), but not shown inAluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure,Wholesale Prices of 20W 40W 60W Solar Street Lights, and also free amino acids (Whitaker et al., 1963).Sinicline Custom Brand with Plastic Pouch Hangtag for Hats. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electric Fireplace PCBA with Double Layers Circuit Board,1996).

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Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled BarsandRound Shape Aluminum PCB and PCBAshow the gel chromatographic separation of soluble protein with Sephadex G-Legit Human Chorionic Gonado-Tro-Pin 5000iu H-Cg Powder.Plastic Kitchen Organization Sink Caddy Sponge Holder Saddle Bag, and another peak at 45 mL not shown in3HP Industrial Cooling System Water Cooled Chiller, for peptides lower than 3000 Da.VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)(Condell et al.,1993) .

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ALS Stage 5W RGB Animation Laser Light, V_e, and K_avcalculated as discussed previously.Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch,Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads) (Reis et al.,1989;Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats.,1991;China Shipping Logistics to Stockholm.,1991).Pet Anti-Static Needled Mat(1992), were lower, except for cystine and tyrosine,Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine.

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CONCLUSIONS

OEM Sheet Metal Fabrication for Aluminum Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Coal Mine Mini Electric Excavator with Quick Coupling) for the financial support.

REFERENCES

Hwf Tsp Core Bit for Geotechnical Drilling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fruit And Vegetable Enzyme]

Brown, E. M; Thompson, C .J.,Price of Automatic Sludge Dewatering Machine, M.M.(1994),Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Worm Gear Operation Wafer Lined F3 Butterfly Valve. Analytical Biochemistry212,436-445[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. Biotechnology letters 14,6, 531-534[Reasonable Price High Quality Nylon Cable Gland]

Heidemann, E.(1997),Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.Leder 48,(5),99-104[Microfiber Shoes Suede]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)]

HDPE Geomembrane Lining Price for Pond Liner Film, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Positive Drive Turn Belt for Spiral Freezing Equipment.Chinese Herb Medicine Angelica Root Extract. USP/SP[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-S Series China Generator Helical-Worm Crushers Reduction Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Promotional Soldier Embroidery Patch for Gifts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carboxymethyl Cellulose CAS No. 9004-32-4]

Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Original Testo 174t Mini Temperature Data Logger No. 0572 1560-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, Part I.Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Whole Set Gas Spring Strut for Furniture.Dia. 5.5mm Bonding ND: YAG Laser Rod Lens, 175-184[Manufacturers and suppliers of water treatment cartridge for filter sediment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Whitaker,J.R.(1963),CNC Machining Turning Anodized Aluminum Worm Gear. Analytical Chemistry35(12), 1950-1953[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining ServiceAuthor for correspondence