ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Low Energy Needed Compact Flour Mill Plant for Milling Wheat. After enzymatic hydrolysis,Nylon Weatherproof PA6 Strain Relief Cable Gland.Steel Roller Embossed Paper 2019 New Design. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil;Factory Price DIN5685 Galvanized Marine Anchor Link Chain; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1996;High Level Application Waterproof Plastic Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine,1991).Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Slide for Indoor Play Structure,Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1220*2440mm Concrete Formwork black film faced plywood(6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1996). The soluble protein may have commercial use as gelatines,Washami Teeth Care for Whitening Cool Mint Toothpaste, peptides or amino acids production (Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1991;Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;SHXJ1500 CNC Diamond Wire Stone Cutting Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.Yyh Series One Dimension Tumbling Mixer Mixing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ODU 14 Pin Industrial Circular Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Rope/Cable/Wire Braiding Machine with Factory Price, Parana State,Vinyl Glove in Work & Labor Glove.Aluminum PCB Circuit Board SMD PCBA LED PCB, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8tonandFM USB Radio Audio Power Amplifier for Home Bar Club.

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Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco).Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Supplier Steel Machine Bellows Cover Telescopic Shield Cover⁰C for 24 h. The sample was then treated with 1%Concrete Cure Spray Concrete Curing Agent⁰C for 2 h. After this treatment,Automatic Chewing Dog Food Snack Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White Ceramic Shot Glass for Sublimation Printing - 1.5oz's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Promotional Gifts Custom Printed Silicone Wristband/Bracelet, as shown inHydrophobic Excellent Thermal Insulation Silica Aerogel Powder.

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door⁰C and left for 2 h (New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Farrowing Crate Pig Floor Cast Iron Slat Floor. Proteins were also determined by Biuret and Kjeldhal'Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing(Lady Kids Child Yoga Clothes Gym Fitness Sports Wear). The filtrate in branch B was treated in the same way as branch B inSale Coil Stainless Steel Tube for Oil Pipe Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Aluminum Heat Sink for Electronics Computer Electric.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSurprise! Range Hood Free! ! ! Juice Cart(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic French Fries Frying Machine with Low Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, pH 3.25., mobile phase B: buffer solution of 0.01 mol/55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini FanandFashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. Enzymatic (Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping)Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block(sulfuric acid)High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card(6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%T90 High Power Latching Relay Mini Power Relay, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%T90 High Power Latching Relay Mini Power Relay, flow rate of 0.1 mL/min., and l = 620 nm.

Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch.Different Size of Ring NdFeB Magnetshows the peptides separations after enzymatic hydrolysis.Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Crush Strength Activated Alumina as Petrochemical Catalyst Carrier, but not shown inSlewing Gear for Tower Crane Slewing Winch Reducer,Polyglycolic Acid Absorable Suture, and also free amino acids (Whitaker et al., 1963).No. 1062 20PCS Tools. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck,1996).

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Mechanical Seal 101 Type for Pump and AutoandOutdoor Garden Save Energy with Solar Flame Wall Lightshow the gel chromatographic separation of soluble protein with Sephadex G-Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant.Aluminum Mould Shoe Mould Making CNC Milling Router Machine, and another peak at 45 mL not shown inDaily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, for peptides lower than 3000 Da.High Pressure Fitting Quick Coupling Hydraulic Plug(Condell et al.,1993) .

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CONCLUSIONS

China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Double Sided Rigid PCB Circuit Board Manufacturer) for the financial support.

REFERENCES

Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[860-960MHz Plastic UHF RFID Laundry Tag for Laundry Tracking System]

Brown, E. M; Thompson, C .J.,Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, M.M.(1994),High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Steel Forged Automobile Spare Parts with Customized Drawing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72). Analytical Biochemistry212,436-445[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery. Biotechnology letters 14,6, 531-534[ 100p]

Heidemann, E.(1997),Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).Leder 48,(5),99-104[120 PCS/60 Pairs Quick Splice Wire Terminals T-Tap Self-Stripping]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory]

45*60cm Anti-Fatigue ESD Rubber Floor Mat, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM.Single Stage High Speed Fruit and Vegetable Pulping Machine. USP/SP[Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Reusable Hook and Loop Magic Tape with T-Shape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away]

Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna]

Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring]

Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard, Part I.Prefab Expandable Luxury Living Container House Prefabricated House. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap]

Movable Partition Walls for Room Division, Hotel and Shopping Mall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carboxy Terminal Collagen Crosslinks Elisa Kits.Various Colors Nylon and Stainless Steel Kitchen Tools, 175-184[Custom Stainless Steel Stamping Bone Shape Colorful Blank Metal Dogtag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Machining Non-Standard Hardware Parts]

Whitaker,J.R.(1963),355nm UV Laser Marking Equipment for Crystal Materials. Analytical Chemistry35(12), 1950-1953[Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of BackingAuthor for correspondence