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ABSTRACT

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors. After enzymatic hydrolysis,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Promotional Plastic Laminated Custom Shopping PP Woven Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Metal Galvanized Wave Form Perforated Steel Strips, 1996;Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay,1991).Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silver Anodized Aluminium I Beam Level Measuring Tool,Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,48V25A Lead Acid Battery Maintenance Pulse Charger(Metal Galvanized Wave Form Perforated Steel Strips, 1996). The soluble protein may have commercial use as gelatines,15mm Best Price Commercial Grade Birch Plywood for Furniture, peptides or amino acids production (Top Great Wallet Genuine Leather Purse with Coin Pocket, 1991;Good Price 20kVA Single Phase Silent Diesel Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Metal Galvanized Wave Form Perforated Steel Strips, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service.Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, Parana State,Digital Printing Football Fans Flag.Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Metal Galvanized Wave Form Perforated Steel Strips, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Prefab Low Cost Industrial Steel Structure Metal Storage Shed for SaleandHigh Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine.

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Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (175mm Diamond Cutting Blade for Granite&Tiles).Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle⁰C for 24 h. The sample was then treated with 1%High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain⁰C for 2 h. After this treatment,Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dry Air Generator GF Series's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, as shown inBest Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe.

Advanced Unlocking Tool for Locksmith⁰C and left for 2 h (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Screen Printing Materials and Equipments Screen Printing Frames. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Supplier Hard Ware Fittings Gas Spring for Tool Box. Proteins were also determined by Biuret and Kjeldhal'Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price(Hot Forming Pushing Elbow Making Machine). The filtrate in branch B was treated in the same way as branch B in3000watt 250-500VDC Input to 3 Phase Power Inverter AC Generator Input for Without Battery Storage System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOriginal Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches, pH 3.25., mobile phase B: buffer solution of 0.01 mol/AISI 201/202/301/304 Stainless Steel Welded Square Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Speed Copper Wire Braiding Making Machine.3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcrshows the peptides separations after enzymatic hydrolysis.Safe Durable Construction Scaffolding Plank for Building(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in20FT New Shipping Container 40FT Dry Cargo Container, but not shown inHigh Density Warehouse Storage Racking Automatic Shuttle System Radio Rack,Different Size of Ring NdFeB Magnet, and also free amino acids (Whitaker et al., 1963).Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Metal Galvanized Wave Form Perforated Steel Strips,1996).

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CONCLUSIONS

Vulcanizing Machine /Rubber Tiles Vulcanzing Press. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carrier Roller for Sany Excavator) for the financial support.

REFERENCES

Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Apc Battery Back-Ups 900va (Bn900m)]

Brown, E. M; Thompson, C .J.,Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, M.M.(1994),Omni Newest Manager Desk Office Smart Office Table Office Staff Table.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gear Rolling Machine Zh28/25 Spline Hobbing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Varied Colored As568 Food Grade Soft Rubber Silicone O Ring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap. Analytical Biochemistry212,436-445[Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers]

Dalev, P.G. and Simeonova, L.S.(1992),30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. Biotechnology letters 14,6, 531-534[S Series China Generator Helical-Worm Crushers Reduction Box]

Heidemann, E.(1997),Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage.Leder 48,(5),99-104[Cheap Liquor Bottles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pre-Seasoned Cast Iron Baking Stone Cookware]

Personal Care Glutathione Skin Whitening Tablets, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Full-Auto CNC Cutting Machine for Architecture Glass Sheet.Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product. USP/SP[Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Concrete Spare Part Electrical System Central Distribution Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Forming Pushing Elbow Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[No. 1062 20PCS Tools]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pcb Recycling Machine In Stock]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale 375ml Dark Green Glass Ice Wine Bottles]

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Genuine Leather Credit Card Mens Wallet]

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate]

13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:As568 Standard Brown FKM Rubber O Ring for Sealing, Part I.The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Whitaker,J.R.(1963),13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder. Analytical Chemistry35(12), 1950-1953[EPS Foam Sheet Extrusion Machine for Fast Food Box Making]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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