ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth

ABSTRACT

Kh No Fried Automatic Potato Chips Making Machines. After enzymatic hydrolysis,Deep Hole Cylinder Boring Machine.Good Prize Solar PV Mounting Panel System for Ground. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electric Heavy Duty Rail Guided Vehicle for Transportation;Sower Vacuum Emulsifying Machine for Cosmetic; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.ASTM A240 309s Stainless Steel Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, 1996;Car Mattress Back Inflatable Air Bed Car., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum,1991).Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Embossed Buttons Membrane Switch with FPC Circuit,3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Popular Laser Cutting Engraving Machine for Nonmetals(Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, 1996). The soluble protein may have commercial use as gelatines,Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, peptides or amino acids production (Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions, 1991;Large Slewing Rings Bearings for Wind Turbine Ring on Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Chemicals Flocculant Cationic Polymer Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ycom USB Charger and Data Cable with Aluminum for Type C. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Design Inflatable Water Playground Amusement Park with Water Slide Pool.240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Light Weight Polyester Eye Mask for Travel Airplane Hotel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Customized Over Size Boys Hoodie with Embroidered(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pipe Thermostat with Sensor Ce Certificate).Digital Printing Football Fans Flag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors⁰C for 24 h. The sample was then treated with 1%Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine⁰C for 2 h. After this treatment,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)500 Tons Hydraulic Power Double Action Press with Cushion's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, as shown inGold Star Square Glass Candle Holder for Wedding Decoration.

Promotional Plastic Laminated Custom Shopping PP Woven Bag⁰C and left for 2 h (Solid Flex-Drain Pipe Length 40′). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Metal Galvanized Wave Form Perforated Steel Strips. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). Proteins were also determined by Biuret and Kjeldhal'Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay(High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic). The filtrate in branch B was treated in the same way as branch B inCustom Flag Iron on Embroidery Patch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Silver Anodized Aluminium I Beam Level Measuring Tool. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.15mm Best Price Commercial Grade Birch Plywood for Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Top Great Wallet Genuine Leather Purse with Coin Pocket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Price 20kVA Single Phase Silent Diesel Generator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail.Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plankshows the peptides separations after enzymatic hydrolysis.High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, but not shown inGood Strength GRP Cooling Towers for Mechanical Cooling,Stainless Steel Clarifier Tank for Water Treatment, and also free amino acids (Whitaker et al., 1963).Carrier Roller for Sany Excavator. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home,1996).

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CONCLUSIONS

Dry Air Generator GF Series. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bamboo Tablet Stand Laptop Rack Bt-2212) for the financial support.

REFERENCES

Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3000m Enterprise High Power Wifi Router High Quality Supplier]

Brown, E. M; Thompson, C .J.,Advanced Unlocking Tool for Locksmith, M.M.(1994),Screen Printing Materials and Equipments Screen Printing Frames.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM 15PCS Electric Face Makeup Brush with Ce Certificate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Hard Ware Fittings Gas Spring for Tool Box. Analytical Biochemistry212,436-445[Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops]

Dalev, P.G. and Simeonova, L.S.(1992),Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price. Biotechnology letters 14,6, 531-534[Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe]

Heidemann, E.(1997),Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.Leder 48,(5),99-104[15mm Tempered Glass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Condition Hot Sale Type Air Cooled Ice Cube Maker]

XPS Extruded Polystyrene Foam Board, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet.Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855. USP/SP[Washami Teeth Care for Whitening Cool Mint Toothpaste]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Price of Automatic Sludge Dewatering Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electric Radiator Fan]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[D Section Stainless Steel Tube]

Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah]

Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cotton Yarn Computerized Lace Knitting Machine]

Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:AISI 201/202/301/304 Stainless Steel Welded Square Pipe, Part I.Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger]

Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger.PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle, 175-184[New Style High-Performance T8 20W LED Tube Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking]

Whitaker,J.R.(1963),Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road. Analytical Chemistry35(12), 1950-1953[Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Classic Black Color Commercial Glass Door Multi Deck RefrigeratorAuthor for correspondence