ARTICLES

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ABSTRACT

Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens. After enzymatic hydrolysis,Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping.Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment;Metal Material and Sports Theme Custom Metal Medal; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Portable Installation and Ce Certification 20g/Hr OEM Air Purifier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), 1996;Full Computer Plain Socks Knitting Machine with High Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil,1991).New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette,Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light(Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), 1996). The soluble protein may have commercial use as gelatines,Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles, peptides or amino acids production (Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel, 1991;Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Made in China Lqa Strap Banding Tool., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New R&D Wire Cable Feeding Winding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Residence Use 220V 50Hz 12kw Portable silent Diesel Generator.Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Carbon Graphite Crucible, Parana State,Flour Making Machine Wheat Maize Flour Mill Machinery.Decorative Aluminum Solid Panel for Commercial Building, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Quality Pillow for Pregnant Wome Body Pregnant Pillow CaseandStainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3).

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Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM 15PCS Electric Face Makeup Brush with Ce Certificate).Di DIN3352 Double Orifice Automatic Air Release Valve Dn65(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Magic Hair Twist   Curl Sponge Brush⁰C for 24 h. The sample was then treated with 1%Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)⁰C for 2 h. After this treatment,Geyi Disposable Laparoscopic Visible Optical Trocar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bimetallic Thermometer Industrial Temperature Gauge, as shown inDisposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops.

19 Network Cabinet with Lockable Rear Door⁰C and left for 2 h (Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,175mm Diamond Cutting Blade for Granite&Tiles. Proteins were also determined by Biuret and Kjeldhal'Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables(New Condition Hot Sale Type Air Cooled Ice Cube Maker). The filtrate in branch B was treated in the same way as branch B inLeafing And Non Leafing Aluminum. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PU-Flex Duct Hose for Transporting Dust, Granules and Shavings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUprigth Touch-Screen Red Wine Chiller(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Washami Teeth Care for Whitening Cool Mint ToothpasteandGroove Type Wood Log Tree Peeling and Debarking Machine. Enzymatic (High Quality CE Digital Display Adjustable Power Supply)Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires(sulfuric acid)Parcel Tape Adhesive OPP Packing Tape(Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Efficiency Non-Expensive Cracking Agent, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Efficiency Non-Expensive Cracking Agent, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Galvanized Roller Shutter Window Torsion Springs) for the financial support.

REFERENCES

Pink French Loop Terry Knit Fabric for Jacket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High-Tech Platen Vulcanizing Press Rubber Machine / Automatic Plate Vulcanizing Press]

Brown, E. M; Thompson, C .J.,Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, M.M.(1994),Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel. Analytical Biochemistry212,436-445[ 316L)]

Dalev, P.G. and Simeonova, L.S.(1992),Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case. Biotechnology letters 14,6, 531-534[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Heidemann, E.(1997),Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600).Leder 48,(5),99-104[Galvanized Welded Wire Mesh Rolls with Square Hole]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[22 High Rubber Mixing Mill]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Quilting Machines Exporter Hot Sale Product]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Temperaturethermocouple Male and Female Plug]

Cute Pink Cashmere Soft Plush Lining Warm Bootie, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3kw 24V 310mm Vertical Agv Drive Wheel Assembly-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle]

Cute Pink Cashmere Soft Plush Lining Warm Bootie,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrical PV DC Solar Cable]

Cute Pink Cashmere Soft Plush Lining Warm Bootie, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sofa Materials PU Leather with Flock Backing, Part I.Three Phase 800mm Plate Fan. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine]

Whitaker,J.R.(1963),Wholesale Genuine Leather Credit Card Mens Wallet. Analytical Chemistry35(12), 1950-1953[Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

175mm Diamond Cutting Blade for Granite&TilesAuthor for correspondence