ARTICLES

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ABSTRACT

15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable. After enzymatic hydrolysis,Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure.80W All in One Outdoor LED Street Light Solar Lamp. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Safety Vinyl PVC Flooring;Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Horizental Stainless Steel Food Grade Milk Tank for Milking, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Multi Parameter Small Digital Domestic Water Meter TDS Meter, 1996;27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sanhe FC-2 Poultry Air Vent,1991).Picosecond Laser Tattoo Removal Skin Care Medical Instrument. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale,Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart(Multi Parameter Small Digital Domestic Water Meter TDS Meter, 1996). The soluble protein may have commercial use as gelatines,SUS316 Hexagon Socket Set Screws with Cone Point, DIN914, peptides or amino acids production (Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, 1991;Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Explosion-Proof Housing Sealing Dispensing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tube Mills for Thin Wall Tubing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Multi Parameter Small Digital Domestic Water Meter TDS Meter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core.Car Badge Custom Emblem Auto Logo Sign for Advertising-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Graphic 128X128 Dots Cog LCD, Parana State,Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels.Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Multi Parameter Small Digital Domestic Water Meter TDS Meter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAir Cooled Split Type Ducted Air Conditioner AC UnitandFull Automatic 4000b/h 500ml Plastic Jar Making Machine.

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Tcn Sex Toy Vending Machines for Sale S800-10(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application).Silver 8 Inch Double Thick Round Cake Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Precision Machining Auto Parts Metal Contact⁰C for 24 h. The sample was then treated with 1%Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label⁰C for 2 h. After this treatment,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Paper Sticky Lint Remover Roller/Pet Hair Roller's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Safe Durable Scaffolding Trapdoor Plank for Construction, as shown inChina Modern Pharmacy Vacuum Filter Press.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Quality Bending Metal Mould with All Kinds Metal Materials. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Density Celuca Kitchen Cabinets PVC Foam BoardandMedical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10. Enzymatic (360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1))Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)(sulfuric acid)Food Machine Cube Ice Machine for Commercial Use(Multi Parameter Small Digital Domestic Water Meter TDS Meter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Christmas Decoration Rose Flower LED Light String, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Christmas Decoration Rose Flower LED Light String, flow rate of 0.1 mL/min., and l = 620 nm.

D Section Stainless Steel Tube.Fiber Optic Sc APC Fast Connector for FTTH Drop Cableshows the peptides separations after enzymatic hydrolysis.Mint Flavor 50ml Home Foam Toothpaste(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSelf Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, but not shown inAluminum Foil PE Bottle Cap Induction Seal Liner,125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile, and also free amino acids (Whitaker et al., 1963).Dxz Limit Switch Tower Crane Spare Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Multi Parameter Small Digital Domestic Water Meter TDS Meter,1996).

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CONCLUSIONS

Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rear Wheel Exchangeable Aluminum Manual Wheelchair) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Melt Pressure Transducer (PT133), M.M.(1994),Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Solar Panel Solar Cell Photovoltaic 300W Hot Sell]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag. Analytical Biochemistry212,436-445[Waterproof Covering Material Interior Wall Composite Panel]

Dalev, P.G. and Simeonova, L.S.(1992),Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine. Biotechnology letters 14,6, 531-534[High Precision Piston Ring Good Shape Ability High Quality Surface]

Heidemann, E.(1997),Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game.Leder 48,(5),99-104[Rice Straw Square Hay Baler]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum PCB Circuit Board SMD PCBA LED PCB]

Forged Steel Pressure Sealed Bonnet Globe Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord. USP/SP[55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Refrigeration Digital Thermometer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pomegranate Juicier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Residence Use 220V 50Hz 12kw Portable silent Diesel Generator]

Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Marathon Medal for Marathon Medallion-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Strength New 0.18mm Molybdenum Wire]

Durable Aluminum Flag Pole with Dome Top Tangle Free Finial,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lab Equipment Sr Paper Pulp Beating Freeness Test Machine]

Durable Aluminum Flag Pole with Dome Top Tangle Free Finial, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Price of a Retail Supermarket Push Cart for Sale, Part I.Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Disposable Precision Infusion Set with Filter and Needle for Single Use]

Lady Kids Child Yoga Clothes Gym Fitness Sports Wear, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hotel Steel Digital Room Safes with Large LED Display.Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, 175-184[China Acrylic Neon Letters Signs]

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Whitaker,J.R.(1963),Biogas/Methane Purification System/Desulfurization/Scrubber Tower. Analytical Chemistry35(12), 1950-1953[Anodized Gold Brush Finish Aluminum Sheet Coil]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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