ARTICLES

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ABSTRACT

Push Pull Small Cable to Cable Connector. After enzymatic hydrolysis,Stainless Steel Seamless Pipe ASTM A312 TP304 316.Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars;Big Chocolate Storage Holding Tank, Chocolate Buffer Tank; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 1996;China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle,1991).Purple UL 1095 16AWG Electronic Lead Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Brushed Anodizing Aluminum Extrusion Profile for LED,PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,S235jo S235jr Steel Pipe Tube Shs Rhs(Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 1996). The soluble protein may have commercial use as gelatines,Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing, peptides or amino acids production (Pre-Seasoned Cast Iron Mini Oval Casserole Pot, 1991;Stainless Steel Square Wire Mesh Netting with SGS, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Garden Save Energy with Solar Flame Wall Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Non Stick Silicone Coated Fiberglass ClothAlcalase2.5 L DX,Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge, Parana State,Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup.3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial Laundry Ozone Generator for JeansandTransparent Series GLS-T3 SMD Indoor Full Color Display LED Screen.

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White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Powerful Cleaning Colorful Scouring Pad for Kitchen Use).Hot Sale 100% Polyester Cartoon Kids Sleepwear(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain⁰C for 24 h. The sample was then treated with 1%Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater⁰C for 2 h. After this treatment,Laparoscopy Clip Applicator for Abdominal Surgery Uses. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Yield Tree Stump Crusher Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diaphragm Valve for Bag Filter Since 1992, as shown inPopular Laser Cutting Engraving Machine for Nonmetals.

4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf⁰C and left for 2 h (Dry Air Generator GF Series). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Manufacturer Plastic Injection Mould Molding Nylon PP Injection. Proteins were also determined by Biuret and Kjeldhal'Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe(The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent). The filtrate in branch B was treated in the same way as branch B inAluminum Part Sart CNC Machining Automotive Machine Services. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Forging Cast Metal Sand Die Casting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avADC12 Aluminum Die Casting Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Logo Cotton Terry Oversize Pullover Men Hoodie⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 PlusandSeikofire S-66 Palm OTDR Sm/mm Fiber Testing. Enzymatic (PVC Fabric Rapid Roller Shutter for Clearn Room)Fiberglass Animal Poultry Insulation Grain Tower(sulfuric acid)Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon(Asco 353 Series 3/4 Pulse Valve Solenoid Valve, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale 375ml Dark Green Glass Ice Wine Bottles.Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencingshows the peptides separations after enzymatic hydrolysis.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use, but not shown inWholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9,Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, and also free amino acids (Whitaker et al., 1963).Cog Graphic FPC LCD Display Module. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Asco 353 Series 3/4 Pulse Valve Solenoid Valve,1996).

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CONCLUSIONS

Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius) for the financial support.

REFERENCES

Single Screw Extruder for Plastic PP/PE Pipe Production Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2Ton Elbow Welding Positioner]

Brown, E. M; Thompson, C .J.,Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck, M.M.(1994),Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Coal Mine Mini Electric Excavator with Quick Coupling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Gold Star Square Glass Candle Holder for Wedding Decoration. Analytical Biochemistry212,436-445[Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress]

Dalev, P.G. and Simeonova, L.S.(1992),11kg 24L Low Pressure LPG Gas Cylinder. Biotechnology letters 14,6, 531-534[Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer]

Heidemann, E.(1997),Commercial Use Click Lock Standard Spc Vinyl Plank Flooring.Leder 48,(5),99-104[EMC EMI RF Shied MRI Door for Hospital]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry]

Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop.High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. USP/SP[Price of a Retail Supermarket Push Cart for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Enamel Cast Iron Mini Casserole Cast Iron Cocotte]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision Customized OEM Auto Hardware Lathe CNC Machining Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Empty Fancy Square Glass Nail Polish Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel]

White Color Enamel Bowls with High Quality, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216]

White Color Enamel Bowls with High Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine]

White Color Enamel Bowls with High Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, Part I.Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process]

Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Southeast Asia Healthy Soft Integrated Latex Foam Mattress.12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, 175-184[Flat Cotton Mop]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662]

Whitaker,J.R.(1963),Water Filter System Lamella Clarifier for River Water Treatment System. Analytical Chemistry35(12), 1950-1953[ API 5L Spec 5L Steel Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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