ARTICLES

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ABSTRACT

High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. After enzymatic hydrolysis,21.5′′ Wall Mount Display Capacitive Touch Monitor.Pagoda Gazebo Tent in Guangzhou for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe;Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Black Small Fridge for Showcase, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), 1996;Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Zircon Butterfly Open Ring Diamond Fashion Woemn Rings,1991).High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Spare Parts Custom Injection Plastic Moulding Manufacturer,Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood(Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), 1996). The soluble protein may have commercial use as gelatines,Kingdomway Brand Vitamin a 1000, peptides or amino acids production (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, 1991;High Speed Roll Plastic T-Shirt Bag Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Black Galvanized Head Stainless Steel Screw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰45*60cm Anti-Fatigue ESD Rubber Floor Mat.Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vinyl Glove in Work & Labor Glove(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Price DIN5685 Galvanized Marine Anchor Link Chain, Parana State,Rechargeable Battery Operated Cooling Folding Electric Fan.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Refrigeration Digital ThermometerandResidence Use 220V 50Hz 12kw Portable silent Diesel Generator.

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6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lab Equipment Sr Paper Pulp Beating Freeness Test Machine).High Level Application Waterproof Plastic Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine⁰C for 24 h. The sample was then treated with 1%Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse⁰C for 2 h. After this treatment,Water Slide for Indoor Play Structure. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1220*2440mm Concrete Formwork black film faced plywood, as shown inDisposable Precision Infusion Set with Filter and Needle for Single Use.

Washami Teeth Care for Whitening Cool Mint Toothpaste⁰C and left for 2 h (Ycom USB Charger and Data Cable with Aluminum for Type C). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. Proteins were also determined by Biuret and Kjeldhal'SHXJ1500 CNC Diamond Wire Stone Cutting Machine(Anodized Gold Brush Finish Aluminum Sheet Coil). The filtrate in branch B was treated in the same way as branch B inCap Automatic Sandblasting Machine USD for Cook Ware Cleaning Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ODU 14 Pin Industrial Circular Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Classical New Coming Plastic Female Sunglasses, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with PrintedandOEM Double Sided Rigid PCB Circuit Board Manufacturer. Enzymatic (Robot of World Robotics Competition)Professional Rope/Cable/Wire Braiding Machine with Factory Price(sulfuric acid)Aluminum PCB Circuit Board SMD PCBA LED PCB(Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159), flow rate of 0.1 mL/min., and l = 620 nm.

Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck.Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valveshows the peptides separations after enzymatic hydrolysis.China Supplier Steel Machine Bellows Cover Telescopic Shield Cover(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table, but not shown inChampagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net,Concrete Cure Spray Concrete Curing Agent, and also free amino acids (Whitaker et al., 1963).Automatic Chewing Dog Food Snack Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Microwave Motion Sensor Module Doppler Radar Detector (HW-M10),1996).

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8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, V_e, and K_avcalculated as discussed previously.Farrowing Crate Pig Floor Cast Iron Slat Floor,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Surgical Power Tool Medical Care Orthopedic Mini Bone Saw) (Reis et al.,1989;Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine.,1991;Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile.,1991).High Quality Aluminum Heat Sink for Electronics Computer Electric(1992), were lower, except for cystine and tyrosine,5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover.

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CONCLUSIONS

Surprise! Range Hood Free! ! ! Juice Cart. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Steel Roller Embossed Paper 2019 New Design) for the financial support.

REFERENCES

Steam Eye Mask Heating One Eye Mask for Sleeping, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Turnbuckle Screws]

Brown, E. M; Thompson, C .J.,Automatic French Fries Frying Machine with Low Price, M.M.(1994),Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns.The Journal of the American Leather Chemists Association (JALCA)89(7)[1.6 Ton Hand Lifting Tool Lever Chain Block]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3.0kw Spindle CNC Router with Certificate for Woodworking]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software. Analytical Biochemistry212,436-445[Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. Biotechnology letters 14,6, 531-534[Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron]

Heidemann, E.(1997),Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping.Leder 48,(5),99-104[High Strength Bolt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace]

Air Bubble Insulation Thermal Building Insulation Material, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block.High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card. USP/SP[10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-T90 High Power Latching Relay Mini Power Relay: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Aluminum Heat Sink for Electronics Computer Electric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[10ml Empty Travel Perfume Glass Roller Bottle Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale]

Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag]

Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset]

Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polyglycolic Acid Absorable Suture, Part I.No. 1062 20PCS Tools. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)]

Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Mould Shoe Mould Making CNC Milling Router Machine.High Pressure Fitting Quick Coupling Hydraulic Plug, 175-184[Big Shot E Light Shr IPL Beauty Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station]

Whitaker,J.R.(1963),High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover. Analytical Chemistry35(12), 1950-1953[Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Snsc Fd30 Side Shift New Diesel Forklift 3tonAuthor for correspondence