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ABSTRACT

Chinese Galvanized Rectangular Tube for Buildings Materials. After enzymatic hydrolysis,China Customized Chilled Cast Iron Roll.IMTEK High Precision and Rigidity Linear Guides for CNC machinery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Fireplace PCBA with Double Layers Circuit Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Shipping Logistics to Stockholm, 1996;Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings,1991).Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Animal Agricultural Damping Horse Stall Rubber Cow Mat,CNC Automatic Laser Wood Cutting Machine Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yc Pure White Nylon Powder for 3D Printing(China Shipping Logistics to Stockholm, 1996). The soluble protein may have commercial use as gelatines,Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats, peptides or amino acids production (Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, 1991;High Quality Liquid Spouted Stand up Pouch with Custom Logo, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Modern Pharmacy Vacuum Filter Press. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Shipping Logistics to Stockholm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰100% Cotton Luxury Printed Bedding Comforter Set.Mouse Pad with Gel Wrist Rest-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Size Christmas Gift Personalized Watch Boxes for Men(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, Parana State,2.0mm Yellow Markers for Fiber Optic Multi Patch Cord.Boho Gold&Silver Antique 3 Circle Hoops Pendant Earring for Women, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Shipping Logistics to Stockholm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2mm Mirror Glass/Coated Glass for LEDandFood Machine Cube Ice Machine for Commercial Use.

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Factory Direct Sale Single Cloth Hanger Hook(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable).2 Round Sash Pulley for Chain - Brass (PVD)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Anodized Aluminum Expanded Metal Mesh Competitive Price⁰C for 24 h. The sample was then treated with 1%Hot DIP Galvanized G2150 D Ring Shackle⁰C for 2 h. After this treatment,Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Round Shape Aluminum PCB and PCBA's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)36PCS LED Gold Matrix Stage Zoom Light, as shown inFRP Water Tank GRP Fishing Tanks From Factory Supply.

Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China⁰C and left for 2 h (China Solar Panel Solar Cell Photovoltaic 300W Hot Sell). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Chicken Cage Mesh Hexagonal Wire Mesh Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,110/220V Handle Mild Steel Welding Machine PCB Board 160A. Proteins were also determined by Biuret and Kjeldhal'Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming(Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855). The filtrate in branch B was treated in the same way as branch B inAuto Fabric Cutting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Enamel Cast Iron Mini Casserole Cast Iron Cocotte.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnergy Saving Small Air Conitioner Air Source Heat Pump Manufacturer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC System Air Duct Line5 Make Machine for HVAC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Titanium Dioxide Anatase for Paper Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hwf Tsp Core Bit for Geotechnical Drilling. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank) for the financial support.

REFERENCES

Price of Automatic Sludge Dewatering Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery]

Brown, E. M; Thompson, C .J.,Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine, M.M.(1994),Worm Gear Operation Wafer Lined F3 Butterfly Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad. Analytical Biochemistry212,436-445[0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag. Biotechnology letters 14,6, 531-534[Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net]

Heidemann, E.(1997),Positive Drive Turn Belt for Spiral Freezing Equipment.Leder 48,(5),99-104[Hf-44t Mining Exploration Core Drilling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Two Layer License Plates with Wide Border Embossed]

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Chinese Herb Medicine Angelica Root Extract.S Series China Generator Helical-Worm Crushers Reduction Box. USP/SP[Horizental Stainless Steel Food Grade Milk Tank for Milking]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Classical Garment Accessories Jeans Button Metal Shank Button]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Iron Stamping-Vehicle Part-Deep Drawing-Shelf Bracket-Sheet Metal Pressing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Etching Rear Trunk Sill Plates for Hyundai Tucson]

Original Testo 174t Mini Temperature Data Logger No. 0572 1560, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Surgical Power Tool Medical Care Orthopedic Mini Bone Saw]

Original Testo 174t Mini Temperature Data Logger No. 0572 1560,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Design Inclined Plane 18W LED Wall Washer Lighting]

Original Testo 174t Mini Temperature Data Logger No. 0572 1560, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Factory stainless Steel Dining Chair OEM Dining Furniture, Part I.New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality]

Whole Set Gas Spring Strut for Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dia. 5.5mm Bonding ND: YAG Laser Rod Lens.CNC Machining Turning Anodized Aluminum Worm Gear, 175-184[RFID Reader Touch Screen Handheld Barcode Scanner Android PDA (C60)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1.6 Ton Hand Lifting Tool Lever Chain Block]

Whitaker,J.R.(1963),China Solar Panel Solar Cell Photovoltaic 300W Hot Sell. Analytical Chemistry35(12), 1950-1953[3.0kw Spindle CNC Router with Certificate for Woodworking]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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