ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Wholesale Black Galvanized Head Stainless Steel Screw. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine

ABSTRACT

45*60cm Anti-Fatigue ESD Rubber Floor Mat. After enzymatic hydrolysis,Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Vinyl Glove in Work & Labor Glove. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Steel Roller Embossed Paper 2019 New Design, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, 1996;Factory Price DIN5685 Galvanized Marine Anchor Link Chain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001),1991).6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Level Application Waterproof Plastic Box,Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse(Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, 1996). The soluble protein may have commercial use as gelatines,Water Slide for Indoor Play Structure, peptides or amino acids production (Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, 1991;1220*2440mm Concrete Formwork black film faced plywood, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Washami Teeth Care for Whitening Cool Mint Toothpaste., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SHXJ1500 CNC Diamond Wire Stone Cutting Machine.Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Foam Concrete Machine/Foamed Concrete Block Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Classical New Coming Plastic Female Sunglasses, Parana State,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine.OEM Double Sided Rigid PCB Circuit Board Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Displayand Jar Opener.

Polyglycolic Acid Absorable Suture

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Robot of World Robotics Competition(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4 Axis CNC Woodworking Machinery Engraver Router).Professional Rope/Cable/Wire Braiding Machine with Factory Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum PCB Circuit Board SMD PCBA LED PCB⁰C for 24 h. The sample was then treated with 1%Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)⁰C for 2 h. After this treatment,Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Supplier Steel Machine Bellows Cover Telescopic Shield Cover's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Concrete Cure Spray Concrete Curing Agent, as shown inEnergy Saving Track Rail Dimmable 15W 20W 30W LED Track Light.

Automatic Chewing Dog Food Snack Machine⁰C and left for 2 h (Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.White Ceramic Shot Glass for Sublimation Printing - 1.5oz. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Promotional Gifts Custom Printed Silicone Wristband/Bracelet. Proteins were also determined by Biuret and Kjeldhal'Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door( 9004-78-8.). The filtrate in branch B was treated in the same way as branch B inElectrical Impact Wrench For Tire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Farrowing Crate Pig Floor Cast Iron Slat Floor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avManufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Surprise! Range Hood Free! ! ! Juice Cart, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steam Eye Mask Heating One Eye Mask for Sleeping, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ODU 14 Pin Industrial Circular Connector) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Black Color Hexagon Bolt with High Quality, M.M.(1994),China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Picc Puncture Care Kit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price. Analytical Biochemistry212,436-445[Hot Sale Safety Vinyl PVC Flooring]

Dalev, P.G. and Simeonova, L.S.(1992),Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag. Biotechnology letters 14,6, 531-534[Tianjin Manufacturer PTFE Coated Butterfly Valve]

Heidemann, E.(1997),High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton.Leder 48,(5),99-104[Professional Wood Processor Log Splitter for Skid Steer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Biomass Drum Wood Chipper with Competitive Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Distribution Box with Changeable Single Cover Metal Floor Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts]

Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Stage High Speed Fruit and Vegetable Pulping Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, Part I.Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Parcel Tape Adhesive OPP Packing Tape]

Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard.Prefab Expandable Luxury Living Container House Prefabricated House, 175-184[Short Tee Key Klamp]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Whitaker,J.R.(1963),Movable Partition Walls for Room Division, Hotel and Shopping Mall. Analytical Chemistry35(12), 1950-1953[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic ProductsAuthor for correspondence