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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price. After enzymatic hydrolysis,Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag.High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, 1996;Single Stage High Speed Fruit and Vegetable Pulping Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board,1991).Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads,Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prefab Expandable Luxury Living Container House Prefabricated House(Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, 1996). The soluble protein may have commercial use as gelatines,Movable Partition Walls for Room Division, Hotel and Shopping Mall, peptides or amino acids production (Carboxy Terminal Collagen Crosslinks Elisa Kits, 1991;Various Colors Nylon and Stainless Steel Kitchen Tools, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;355nm UV Laser Marking Equipment for Crystal Materials., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Separating Gold Brass Zippers for Sale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Refrigeration Digital Thermometer.BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mini Small Garden Excavator Digging Work(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars).Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Offshore Drilling Platform Air Winch for Marine Oilfields⁰C for 24 h. The sample was then treated with 1%Mass Stock Yl10.2 Carbide Rods for Milling 45HRC⁰C for 2 h. After this treatment,Heavy Industry Electric Transport Vehicle on Rails. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manual Interlocking Brick Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve, as shown inRound Shape Aluminum PCB and PCBA.

Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing⁰C and left for 2 h (3HP Industrial Cooling System Water Cooled Chiller). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile. Proteins were also determined by Biuret and Kjeldhal'Metal Gold Marathon Running Sport Medal for Promotion Gift(355nm UV Laser Marking Equipment for Crystal Materials). The filtrate in branch B was treated in the same way as branch B inLuxury Gold Color Customized Clear Empty Plastic Recycling Lotion/Cream/Spray/Gel Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Security Shopping Trolley Baby Seat for Supermarket Cart. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDesktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Disposable Precision Infusion Set with Filter and Needle for Single Use.LED Emergency Exit Lightsshows the peptides separations after enzymatic hydrolysis.Low Price Sweep Frequency Response Analyzer for Power Transformer Test(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Caps Making Machine / Injection Molding Machine, but not shown inFactory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, and also free amino acids (Whitaker et al., 1963).Stainless Steel Mechanic Parts for Lock Accessories with MIM Process. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM,1996).

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CONCLUSIONS

Supplier V Wedge Wire Johnson Water Well Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes) for the financial support.

REFERENCES

Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Efficiency Abrasive Cup Flap Disc Grinding Wheel]

Brown, E. M; Thompson, C .J.,10A MPPT Solar Controllers PV 60V for off-Grid, M.M.(1994),100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears.The Journal of the American Leather Chemists Association (JALCA)89(7)[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole. Analytical Biochemistry212,436-445[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Dalev, P.G. and Simeonova, L.S.(1992),60ml 100ml Customized Glass Jar for Spice, Candle, Candy. Biotechnology letters 14,6, 531-534[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Air Cooled Split Type Ducted Air Conditioner AC Unit]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mesh Bag Packing 20kg Pure White Garlic: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Metal Stamping Electric Part Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Made in China Lqa Strap Banding Tool]

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Supreme 524 Mv Sea Kayak,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China Modern Pharmacy Vacuum Filter Press]

Supreme 524 Mv Sea Kayak, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), Part I.Diesel Generator Parts AVR Ki-Davr-95s for Kipor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Folding Play Mat Crawl Play Mat]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Dbry-320 Christmas Stickers Labels Printing Machine]

Whitaker,J.R.(1963),Snsc Fd30 Side Shift New Diesel Forklift 3ton. Analytical Chemistry35(12), 1950-1953[High Density Celuca Kitchen Cabinets PVC Foam Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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