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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Flat Car Sun Roof Tent Awning. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Classic Black Color Commercial Glass Door Multi Deck Refrigerator

ABSTRACT

Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits. After enzymatic hydrolysis,12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026).Pneumatic Control Shuttle Valve NPT Thread. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing;OEM 15PCS Electric Face Makeup Brush with Ce Certificate; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Styles Fashion Erotic Women Underwear Sexy Lingerie, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, 1996;Stand Hot and Cold Normal Water Dispenser with Refrigerator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Construction Welding Rods Electrode E6013 E7018 E7016,1991).High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular,Lydite Fence Reel Reel with Extension Arms for Farm Fencing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio(on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, 1996). The soluble protein may have commercial use as gelatines,Customized 3D Medal for Religious Medal Gift, peptides or amino acids production (Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, 1991;Dbry-320 Christmas Stickers Labels Printing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad.Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, Parana State,Classic Black Color Commercial Glass Door Multi Deck Refrigerator.O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Selling Used Plastic Single Shaft Shredder for Recycling with Weg MotorandMust 4kw 2HP Pump Power Inverter Ags Bts Available.

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20FT New Shipping Container 40FT Dry Cargo Container⁰C and left for 2 h (Varied Colored As568 Food Grade Soft Rubber Silicone O Ring). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone. Proteins were also determined by Biuret and Kjeldhal'High Pressure Braided Rubber Oil Hose(Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers). The filtrate in branch B was treated in the same way as branch B in16m 18m Construction Use Mini Towable Articulated Boom Lift. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Monochrome Graphic Industrial Control LCD Display Module 128X64.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SMD LED Light-Operated USB Rechargeble Flashlight. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHolland Welded Wire Mesh Euro Fencing /Euro Fence for Garden(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chinese 1325 Ce Certificated CNC Engraving Router for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Oil Heating Reactor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%, V_e, and K_avcalculated as discussed previously.Evaporator Finned Tube,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device) (Reis et al.,1989;Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules.,1991;Stand Hot and Cold Normal Water Dispenser with Refrigerator.,1991).Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(1992), were lower, except for cystine and tyrosine,100%Polyester Spandex Hotel Chair Cover (DPH7022).

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CONCLUSIONS

Factory Supply Perforated Metal Mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Bright Polished Mirror Sheet) for the financial support.

REFERENCES

CNC Turning Center, CNC Horizontal Turning Milling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Factory Door Jamb Frames]

Brown, E. M; Thompson, C .J.,Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, M.M.(1994),Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. Analytical Biochemistry212,436-445[Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure]

Dalev, P.G. and Simeonova, L.S.(1992),Vitamin D3 Fat-Solubility Water-Solubility Feed Additive. Biotechnology letters 14,6, 531-534[Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence]

Heidemann, E.(1997),Factory Wholesale Zinc Plated Carbon Steel Hex Nut.Leder 48,(5),99-104[Hotel Lobby Public Furniture Small Reception Desk for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars]

Partial Thread Steel/Iron Hex Head Self Tapping Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern.Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. USP/SP[Round Shape Aluminum PCB and PCBA]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3HP Industrial Cooling System Water Cooled Chiller]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Cheese Making Machine Electric: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[355nm UV Laser Marking Equipment for Crystal Materials]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.usbcmaster.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads]

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100W Dimmable LED Corn Bulb with Meanwell Driver-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LED Emergency Exit Lights]

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Caps Making Machine / Injection Molding Machine]

Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet and Animal Food Extrusion Machine, Part I.Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller]

Hexagonal Wire Mesh Chicken Wire Mesh High Quality, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),V-32 New Special UPS Solar Battery Cabinet Box Case.4-14 Manual Staple Gun with Staples for Hand Tools, 175-184[Aluminum 6061 7075 CNC Turning Part]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

Whitaker,J.R.(1963),Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan. Analytical Chemistry35(12), 1950-1953[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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