ARTICLES

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ABSTRACT

Explosion-Proof Housing Sealing Dispensing Machine. After enzymatic hydrolysis,Tube Mills for Thin Wall Tubing.Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Car Badge Custom Emblem Auto Logo Sign for Advertising;2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, 1996;Rear Wheel Exchangeable Aluminum Manual Wheelchair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow,1991).Graphic 128X128 Dots Cog LCD. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen,Tcn Sex Toy Vending Machines for Sale S800-10,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Silver 8 Inch Double Thick Round Cake Board(Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, 1996). The soluble protein may have commercial use as gelatines,High Precision Machining Auto Parts Metal Contact, peptides or amino acids production (Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label, 1991;Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Paper Sticky Lint Remover Roller/Pet Hair Roller., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Safe Durable Scaffolding Trapdoor Plank for Construction.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Various Sublimation Blank Home Supply Fabric Bath Towel etc.High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails, Parana State,Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC.Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStainless Steel Filter StrainerandCheap Price Paper Straws Individually Wrapped Drinking Straw.

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Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh).Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1)⁰C for 24 h. The sample was then treated with 1%Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)⁰C for 2 h. After this treatment,Food Machine Cube Ice Machine for Commercial Use. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Christmas Decoration Rose Flower LED Light String's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)D Section Stainless Steel Tube, as shown inLaser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System.

Mint Flavor 50ml Home Foam Toothpaste⁰C and left for 2 h (XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dxz Limit Switch Tower Crane Spare Parts. Proteins were also determined by Biuret and Kjeldhal'OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock(96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood). The filtrate in branch B was treated in the same way as branch B inSilicone Radiator Hose. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High-End Business Magnet Leather Ring Binder Notebook.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Bearing with Housing Bearing / Pillow Block Bearing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800, pH 3.25., mobile phase B: buffer solution of 0.01 mol/The Most Competitive Sodium Gluconate for Water Reducer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine.Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)shows the peptides separations after enzymatic hydrolysis.Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Mechanic Parts for Lock Accessories with MIM Process, but not shown inHigh Quality MPO Singlemode Duplex Fiber Optic Adapter,Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, and also free amino acids (Whitaker et al., 1963).Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006,1996).

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Price of a Retail Supermarket Push Cart for Sale, V_e, and K_avcalculated as discussed previously.Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder,Lady Kids Child Yoga Clothes Gym Fitness Sports Wear. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube) (Reis et al.,1989;Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label.,1991;Rear Wheel Exchangeable Aluminum Manual Wheelchair.,1991).Hotel Steel Digital Room Safes with Large LED Display(1992), were lower, except for cystine and tyrosine,Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture.

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CONCLUSIONS

Biogas/Methane Purification System/Desulfurization/Scrubber Tower. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Popular Custom School Uniform Woven Badge Patch with Merrow Border, M.M.(1994),1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. Analytical Biochemistry212,436-445[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

Dalev, P.G. and Simeonova, L.S.(1992),Electrical Stationary Hoist Tower Crane 500kg Motor. Biotechnology letters 14,6, 531-534[Agriculture Film for Corn Silage Wrap Baler]

Heidemann, E.(1997),Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage.Leder 48,(5),99-104[abs box plc]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange.Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. USP/SP[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flow Wrapper/ Bakery Moon Cake Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wet Wipes Bucket Sealing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gym Fitness Equipment Adjustable Weight Bench]

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb]

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card]

Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reusable Metal Stainless Steel Drinking Straws, Part I.Designed Steel Frame Factory Building. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Reusable Metal Stainless Steel Drinking Straws]

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table.Scouring Pads Making Machine for Brush King, 175-184[Multi-Function Gear Ring Single-Section Flexible Stainless Steel Pipe Coupling]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine]

Whitaker,J.R.(1963),Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor. Analytical Chemistry35(12), 1950-1953[ D1400]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)Author for correspondence