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ABSTRACT

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. After enzymatic hydrolysis,High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou.High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Motorcycle Reflector Injection Plastic Parts Mould Maker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, 1996;Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51,1991).Customized Souvenirs Tin Metal Number & Name Plate for Car. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System,Molasses Fuel Ethanol Distillation Plant,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pond Self Priming Booster Pressure Water Pump with Check Valve(O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, 1996). The soluble protein may have commercial use as gelatines,Banded Type Tee Used on Oil, peptides or amino acids production (20FT New Shipping Container 40FT Dry Cargo Container, 1991;High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Pressure Braided Rubber Oil Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Monochrome Graphic Industrial Control LCD Display Module 128X64. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰SMD LED Light-Operated USB Rechargeble Flashlight.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:1jo4 Hydraulic Adaptors for Hose Fittings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh).Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting⁰C for 24 h. The sample was then treated with 1%Toilet Roll Tissue Wrapping Machine with Auto - Counting⁰C for 2 h. After this treatment,Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Orange Monofilament Fishing Tackle Nylon Fish Net's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), as shown inANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator.

By6-8-200-L Automatic Doypack Premade Pouch Packing Machine⁰C and left for 2 h (Parcel Tape Adhesive OPP Packing Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Diameter 1300mm Lifting Electro Magnet for Cast Iron. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. Proteins were also determined by Biuret and Kjeldhal'Evaporator Finned Tube(Single Screw Extruder for Plastic PP/PE Pipe Production Line). The filtrate in branch B was treated in the same way as branch B inHuman Pathology Prepared Slides Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100%Polyester Spandex Hotel Chair Cover (DPH7022)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Turning Center, CNC Horizontal Turning Milling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator) for the financial support.

REFERENCES

Forged Steel Pressure Sealed Bonnet Globe Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ge Bearing Lubricated Stainless Steel Spherical Bearings Ge50es]

Brown, E. M; Thompson, C .J.,Plastic Injection Moulding Machine Supplier / Factory /Manufacturer, M.M.(1994),Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels.The Journal of the American Leather Chemists Association (JALCA)89(7)[Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[36PCS LED Gold Matrix Stage Zoom Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable. Analytical Biochemistry212,436-445[Glow Powder for Luminous Coating]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure. Biotechnology letters 14,6, 531-534[Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks]

Heidemann, E.(1997),80W All in One Outdoor LED Street Light Solar Lamp.Leder 48,(5),99-104[Proportional Motorized Ball Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids]

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hot Sale Safety Vinyl PVC Flooring.Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation. USP/SP[High Quality Welding Structure]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fiberglass Animal Poultry Insulation Grain Tower]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Horizental Stainless Steel Food Grade Milk Tank for Milking: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Supreme 524 Mv Sea Kayak]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.jmheatexchanger.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory]

Multi Parameter Small Digital Domestic Water Meter TDS Meter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels]

Multi Parameter Small Digital Domestic Water Meter TDS Meter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit]

Multi Parameter Small Digital Domestic Water Meter TDS Meter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sanhe FC-2 Poultry Air Vent, Part I.Picosecond Laser Tattoo Removal Skin Care Medical Instrument. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849.Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart, 175-184[600V 24AWG Teflon FEP Insulated High Temperature Wire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light]

Whitaker,J.R.(1963),SUS316 Hexagon Socket Set Screws with Cone Point, DIN914. Analytical Chemistry35(12), 1950-1953[Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Diameter 1300mm Lifting Electro Magnet for Cast IronAuthor for correspondence