ARTICLES

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ABSTRACT

Security Shopping Trolley Baby Seat for Supermarket Cart. After enzymatic hydrolysis,Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24.Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel;Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manual Foot Pedal Hydraulic Pump, 1996;860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women,1991).CH Series Pid Intelligent Temperature Controller CH402. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory,Disposable Precision Infusion Set with Filter and Needle for Single Use,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Low Price Sweep Frequency Response Analyzer for Power Transformer Test(Manual Foot Pedal Hydraulic Pump, 1996). The soluble protein may have commercial use as gelatines,Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, peptides or amino acids production (Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, 1991;High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mining Machinery Sand Vibrating Sieve for River Sand., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PRO Audio Loudspeaker PA Speaker System Line Array Speaker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manual Foot Pedal Hydraulic Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Palet Coil Nail Welding Machine/Nail Making Machine.Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality MPO Singlemode Duplex Fiber Optic Adapter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, Parana State,12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Manual Foot Pedal Hydraulic Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRechargeable Battery Operated Cooling Folding Electric FanandTelescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop.

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60ml 100ml Customized Glass Jar for Spice, Candle, Candy(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Sw Type BOPP Packaging Adhesive Tape Coating Machine).Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line⁰C for 24 h. The sample was then treated with 1%Factory Outlet Automatic Grain Tower Dryer Drying Equipment⁰C for 2 h. After this treatment,Mesh Bag Packing 20kg Pure White Garlic. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Supreme 524 Mv Sea Kayak's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Trunk Organizer Foldable Water Proof, as shown inUpholstery Ribbon Christmas Tape.

100%Polyester Burnout Velvet Sofa Fabric (pineapple grid)⁰C and left for 2 h (Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Diesel Generator Parts AVR Ki-Davr-95s for Kipor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gld Brand Padlock Brass Popular in 5 Continents  . Proteins were also determined by Biuret and Kjeldhal'No Lead Bronze Flange Manufacturer(12 Bottles Portable Home Ues Thermoelectric Wine Chiller). The filtrate in branch B was treated in the same way as branch B inGMP Standard Chromium Enriched Yeast Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Snsc Fd30 Side Shift New Diesel Forklift 3ton. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSynthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Laptop Bag College Men Backpack Leather Back Pack Bags⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pet Dog Grooming Rake Dematting Tool Shedding Comb, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Apparel Applique Round Patch School Club Embroidery Collar Patch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fused Quartz Powder 325mesh 500mesh 1250meshandFarm Tools and Equipment 7ton Backhoe Loader Construction Machinery. Enzymatic (CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058)Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror(sulfuric acid)Automatic Food Metal Detector Machine for Production Line(Manual Foot Pedal Hydraulic Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Multi-Functional Backpack Manual 52cc Brush Cutter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Multi-Functional Backpack Manual 52cc Brush Cutter, flow rate of 0.1 mL/min., and l = 620 nm.

Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)shows the peptides separations after enzymatic hydrolysis.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, but not shown in X Ray Room Door,Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm, and also free amino acids (Whitaker et al., 1963).White Cotton Satin Hotel Duvet Cover (DPFB8055). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manual Foot Pedal Hydraulic Pump,1996).

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Electrical PV DC Solar Cable, V_e, and K_avcalculated as discussed previously.Tomato Bubble and High Pressure Washing Machine,Upholstery Ribbon Christmas Tape. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns) (Reis et al.,1989;Stainless Steel Mechanic Parts for Lock Accessories with MIM Process.,1991;860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag.,1991).Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate(1992), were lower, except for cystine and tyrosine,2+1 Flavors Soft Ice Cream Machine with Pre-Cooling.

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CONCLUSIONS

Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles) for the financial support.

REFERENCES

40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lora Intelligent Lighting Control System for Streetlight/Tunnel Light (LoRa)]

Brown, E. M; Thompson, C .J.,Best Sale Low Price Household Air freshener Spray, M.M.(1994),Factory Supplied High Strength Marine Anchor Chain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Explosion-Proof Housing Sealing Dispensing Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Easy Operational Cup Cake Filling Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Humidity Refrigeration Digital Controller Jsd-100+. Analytical Biochemistry212,436-445[Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation]

Dalev, P.G. and Simeonova, L.S.(1992),Elegant Europe Dessign Pillow Top Mattress. Biotechnology letters 14,6, 531-534[Tomato Bubble and High Pressure Washing Machine]

Heidemann, E.(1997),8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8).Leder 48,(5),99-104[Sanhe FC-2 Poultry Air Vent]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable PS Foam Fast Food Box Making Machine]

Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts.Modern 4 Person Workstation with Computer Desk Modular Office Furniture. USP/SP[Cheap Gabion Wire Mesh Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Various Sublimation Blank Home Supply Fabric Bath Towel etc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heat Press Machine 8in1]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer Customized Plastic Injection Molding]

Women Fashion Winter V Neck Long Sleeve Clothes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine]

Women Fashion Winter V Neck Long Sleeve Clothes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine]

Women Fashion Winter V Neck Long Sleeve Clothes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3D CNC Router for Wood Cutting Machine, Part I.Stainless Steel Screens / Wedge Wire Filter Element. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machining Service High Precision CNC Mechanical Parts]

Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),EPS Foam Sheet Extrusion Machine for Fast Food Box Making.Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, 175-184[Dual-Glass Module]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500t Plywood Hot Press Woodworking Machine (12-25 layers)]

Whitaker,J.R.(1963),Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah. Analytical Chemistry35(12), 1950-1953[Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Precision Customized OEM Auto Hardware Lathe CNC Machining PartsAuthor for correspondence