ARTICLES

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ABSTRACT

Southeast Asia Healthy Soft Integrated Latex Foam Mattress. After enzymatic hydrolysis,12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.Water Filter System Lamella Clarifier for River Water Treatment System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C);Cabinet Shelf Support Pin Furniture Hardware Fittings; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Reasonable Price High Quality Nylon Cable Gland, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, 1996;Disposable Anesthesia Breathing Circuit Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type,1991).Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126,Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Counter-Clip Fluorine-Lined Butterfly Valve(Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, 1996). The soluble protein may have commercial use as gelatines,DC Brushless Motor for CNC Machine with ISO9001, peptides or amino acids production (Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, 1991;Road Safety Warning Barricade LED Solar Powered Blinking Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer Customized Plastic Injection Molding., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine.60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Solar Energy Storad Power Generation System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Galvanized Eye Grab Bend Hook.High Yield Tree Stump Crusher Machineshows the peptides separations after enzymatic hydrolysis.Transparent LED Display P3.91 Glass Wall LED Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder, but not shown inWater Meter Spare Parts Rubber Rings for Sealing,Surgical Power Tool Medical Care Orthopedic Mini Bone Saw, and also free amino acids (Whitaker et al., 1963).Micro Small Plastic Product Injection Molding Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan,1996).

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CONCLUSIONS

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ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility) for the financial support.

REFERENCES

33kv Dry Type Resin Casted Distribution Transformer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality PVC Resin with Low Price]

Brown, E. M; Thompson, C .J.,Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27, M.M.(1994),Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate.The Journal of the American Leather Chemists Association (JALCA)89(7)[1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale 25kw Grid Tied Home Solar Panel System Kit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Size 000 Clear Pullulan Vegetarian Capsules. Analytical Biochemistry212,436-445[Manual Interlocking Brick Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Sinicline 2018 Hot Sell Custom Printing Thank You Cards. Biotechnology letters 14,6, 531-534[Single Embossed Buttons Membrane Switch with FPC Circuit]

Heidemann, E.(1997),Black Red Open Net Spandex Nylon Sexy Lingerie.Leder 48,(5),99-104[Outdoor Luggage Duffel Shoulder Gym Travel Sports Bag for Men]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball]

Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting.Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor. USP/SP[Polypropylene Woven Bag for Sludge Dewatering]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED Emergency Exit Lights: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Folding  Platform Hand  Trolley ]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bath Robe with 100% Cotton for Hotel Room Using]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sophisticated Durable Membrane Panel/Panel with 3m Glue]

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Car Mattress Back Inflatable Air Bed Car]

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Decorative Aluminum Solid Panel for Commercial Building]

Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine, Part I.Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Human Body Thermometer]

3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic.Leisure Men′s Waterproof Strap Light Cross Body Anti-Theft Chest Bag, 175-184[Car Daytime Running Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[35 Degree Escalator with Auto Start Function in Parallel]

Whitaker,J.R.(1963),Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels. Analytical Chemistry35(12), 1950-1953[CH Series Pid Intelligent Temperature Controller CH402]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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