ARTICLES

Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine

Baby Safety Dining ChairEdivaldo E.Garcia^I; Cláudia C.M.Black Small Fridge for Showcase^I; Ariovaldo C.Martins^II; Gisele O. Rocha^II;Jorge Nozaki^{Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52}

^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Microwave Motion Sensor Module Doppler Radar Detector (HW-M10). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile

ABSTRACT

Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. After enzymatic hydrolysis,High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation.Spare Parts Custom Injection Plastic Moulding Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill;96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Kingdomway Brand Vitamin a 1000, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, 1996;High Speed Roll Plastic T-Shirt Bag Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine,1991).Wholesale Black Galvanized Head Stainless Steel Screw. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine,45*60cm Anti-Fatigue ESD Rubber Floor Mat,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints(Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, 1996). The soluble protein may have commercial use as gelatines,Vinyl Glove in Work & Labor Glove, peptides or amino acids production (Low Energy Needed Compact Flour Mill Plant for Milling Wheat, 1991;Nylon Weatherproof PA6 Strain Relief Cable Gland, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Roller Embossed Paper 2019 New Design., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price DIN5685 Galvanized Marine Anchor Link Chain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001).6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Level Application Waterproof Plastic Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging MachineAlcalase2.5 L DX,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Water Slide for Indoor Play Structure); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

1220*2440mm Concrete Formwork black film faced plywood, Parana State,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile.Washami Teeth Care for Whitening Cool Mint Toothpaste, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustomized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal LabelandDigital Printing Football Fans Flag.

High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet

Film Roll Soap Liquid Packing Machine for Sachet

Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light).Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SHXJ1500 CNC Diamond Wire Stone Cutting Machine⁰C for 24 h. The sample was then treated with 1%Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag⁰C for 2 h. After this treatment,2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yyh Series One Dimension Tumbling Mixer Mixing Machine, as shown inAdhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle.

ODU 14 Pin Industrial Circular Connector⁰C and left for 2 h (Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Classical New Coming Plastic Female Sunglasses. Proteins were also determined by Biuret and Kjeldhal'OEM Double Sided Rigid PCB Circuit Board Manufacturer(PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector). The filtrate in branch B was treated in the same way as branch B inMen Elegant Watch. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Robot of World Robotics Competition.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Rope/Cable/Wire Braiding Machine with Factory Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum PCB Circuit Board SMD PCBA LED PCB(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Concrete Cure Spray Concrete Curing Agent, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa SetandAutomatic Chewing Dog Food Snack Machine. Enzymatic (White Ceramic Shot Glass for Sublimation Printing - 1.5oz)Promotional Gifts Custom Printed Silicone Wristband/Bracelet(sulfuric acid)Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door(Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, flow rate of 0.1 mL/min., and l = 620 nm.

Farrowing Crate Pig Floor Cast Iron Slat Floor.Wholesale Black Galvanized Head Stainless Steel Screwshows the peptides separations after enzymatic hydrolysis.Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTop Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, but not shown inFengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag,High Quality Aluminum Heat Sink for Electronics Computer Electric, and also free amino acids (Whitaker et al., 1963).5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine,1996).

Galvanized Steel Frame PC Board Greenhouse for Planting

Custom Made Colorful Sublimation Womens Yoga Pants Wholesale

Automatic French Fries Frying Machine with Low Priceand3.5ton Chinese Engine Gasoline Forklift (FG35T)show the gel chromatographic separation of soluble protein with Sephadex G-Surprise! Range Hood Free! ! ! Juice Cart.Steam Eye Mask Heating One Eye Mask for Sleeping, and another peak at 45 mL not shown in Art Painting (abstract sailing oil painting), for peptides lower than 3000 Da.Automatic French Fries Frying Machine with Low Price(Condell et al.,1993) .

Pathmate Stone Mold

Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro

Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, V_e, and K_avcalculated as discussed previously.55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software,Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pre-Seasoned Cast Iron Mini Oval Casserole Pot) (Reis et al.,1989;Low Energy Needed Compact Flour Mill Plant for Milling Wheat.,1991;High Speed Roll Plastic T-Shirt Bag Making Machine.,1991).Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping(1992), were lower, except for cystine and tyrosine,Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block.

Micro Small Plastic Product Injection Molding Making Machine

Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine

CONCLUSIONS

High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Water Slide for Indoor Play Structure) for the financial support.

REFERENCES

T90 High Power Latching Relay Mini Power Relay, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[ruichirobot.com]

Brown, E. M; Thompson, C .J.,Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, M.M.(1994),Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Striped Compression Soccer Socks for Adults]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Price Sweep Frequency Response Analyzer for Power Transformer Test]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polyglycolic Acid Absorable Suture. Analytical Biochemistry212,436-445[China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA]

Dalev, P.G. and Simeonova, L.S.(1992),No. 1062 20PCS Tools. Biotechnology letters 14,6, 531-534[Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody]

Heidemann, E.(1997),Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant.Leder 48,(5),99-104[Fiber Optic Amplifier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services]

Black Small Fridge for Showcase, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminum Mould Shoe Mould Making CNC Milling Router Machine.High Pressure Fitting Quick Coupling Hydraulic Plug. USP/SP[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cinema Room Furniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

Refrigeration Digital Temperature Controller Stc-100A, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

Refrigeration Digital Temperature Controller Stc-100A,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing]

Refrigeration Digital Temperature Controller Stc-100A, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies, Part I.Black Color Hexagon Bolt with High Quality. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Non Standard U Channel Steel]

China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price.Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, 175-184[Black Mounts Metal Folding Studio Monitor Stand]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Speed Copper Wire Braiding Making Machine]

Whitaker,J.R.(1963),High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. Analytical Chemistry35(12), 1950-1953[ Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Supplier Granular Coal Based Activated Carbon Filter MeidaAuthor for correspondence