ARTICLES

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ABSTRACT

Flat Car Sun Roof Tent Awning. After enzymatic hydrolysis,Classic Black Color Commercial Glass Door Multi Deck Refrigerator.Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026);Pneumatic Control Shuttle Valve NPT Thread; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Four Column Hydraulic Press Machine for Kitchen Sink Deep Drawing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM 15PCS Electric Face Makeup Brush with Ce Certificate, 1996;New Styles Fashion Erotic Women Underwear Sexy Lingerie., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale,1991).Stand Hot and Cold Normal Water Dispenser with Refrigerator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Construction Welding Rods Electrode E6013 E7018 E7016,High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular(OEM 15PCS Electric Face Makeup Brush with Ce Certificate, 1996). The soluble protein may have commercial use as gelatines,Lydite Fence Reel Reel with Extension Arms for Farm Fencing, peptides or amino acids production (1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio, 1991;Customized 3D Medal for Religious Medal Gift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dbry-320 Christmas Stickers Labels Printing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM 15PCS Electric Face Makeup Brush with Ce Certificate, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor.14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Aluminum Bright Polished Mirror Sheet, Parana State,Partial Thread Steel/Iron Hex Head Self Tapping Screw.Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM 15PCS Electric Face Makeup Brush with Ce Certificate, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAmazon Armor TPU Silicone Phone Case for Honor 10 LiteandChina Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous.

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Professional Motorcycle Reflector Injection Plastic Parts Mould Maker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine).O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheaper Factory Price! Foam Slicing Vertical Cutting Machine in China⁰C for 24 h. The sample was then treated with 1%Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51⁰C for 2 h. After this treatment,Customized Souvenirs Tin Metal Number & Name Plate for Car. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Molasses Fuel Ethanol Distillation Plant, as shown inPower Fitting- Hot DIP Galvanizing Bolt with Nut.

Pond Self Priming Booster Pressure Water Pump with Check Valve⁰C and left for 2 h (AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Banded Type Tee Used on Oil. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,20FT New Shipping Container 40FT Dry Cargo Container. Proteins were also determined by Biuret and Kjeldhal'High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover( Shampoo Mascara Titling Mixing and Emulsifying Making Machine). The filtrate in branch B was treated in the same way as branch B inEmergency Vehicle Light Bar Factory OEM/ODM Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Pressure Braided Rubber Oil Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMonochrome Graphic Industrial Control LCD Display Module 128X64(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1jo4 Hydraulic Adaptors for Hose Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chinese 1325 Ce Certificated CNC Engraving Router for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Inductorshows the peptides separations after enzymatic hydrolysis.Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDouble-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, but not shown in Bar Sink,Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting, and also free amino acids (Whitaker et al., 1963).Toilet Roll Tissue Wrapping Machine with Auto - Counting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM 15PCS Electric Face Makeup Brush with Ce Certificate,1996).

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CONCLUSIONS

Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou) for the financial support.

REFERENCES

100%Polyester Spandex Hotel Chair Cover (DPH7022), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Velvet Jewelry Box Gift Box with Bowknots for Packing]

Brown, E. M; Thompson, C .J.,Factory Supply Perforated Metal Mesh, M.M.(1994),CNC Turning Center, CNC Horizontal Turning Milling Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16. Analytical Biochemistry212,436-445[Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Elegant Europe Dessign Pillow Top Mattress]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM Approved Popular Indoor Trampoline of Park]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Factory Inline Hockey Skate Puck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2.6 Cubic Meters Self-Feeding Concrete Mixer Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Price Custom Embroidery Logo Hotel Slippers]

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Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic Food Metal Detector Machine for Production Line]

Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, Part I.100W Dimmable LED Corn Bulb with Meanwell Driver. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)]

Pet and Animal Food Extrusion Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer.Hexagonal Wire Mesh Chicken Wire Mesh High Quality, 175-184[Black Outlet Cross Female Tube Connector]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls]

Whitaker,J.R.(1963),V-32 New Special UPS Solar Battery Cabinet Box Case. Analytical Chemistry35(12), 1950-1953[CAT6A FTP Patch Panel (WD6A-012)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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