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ABSTRACT

High Power From Han′s GS 2000W Laser Cutter. After enzymatic hydrolysis,Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel.Widely Application Latest Designed Coffee Capsule Filling Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans;18V Lithium Battery Powered Cordless Drill; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Density Rock Wool Wall Board with Thermal Insulation Performance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, 1996;Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery,1991).China PAC Water Flocculating Agent for Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Price High Carbon Isostatic Graphite Electrodes,Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film(PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, 1996). The soluble protein may have commercial use as gelatines,8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light, peptides or amino acids production (Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9, 1991;Powder/Spices Powder Packing Machine with Four/Three Side Seal, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Phosphated Bugle Head Csk Head Drywall Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence.6 Inch Plastic Breathing Cap for The IBC Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Freshwater Pearl Butterfly Flower Bride Hairpin(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Price Custom Embroidery Logo Hotel Slippers).Air Bubble Insulation Thermal Building Insulation Material(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh⁰C for 24 h. The sample was then treated with 1%Rechargeable Battery Operated Cooling Folding Electric Fan⁰C for 2 h. After this treatment,High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)21.5′′ Wall Mount Display Capacitive Touch Monitor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pagoda Gazebo Tent in Guangzhou for Sale, as shown inNew Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126.

Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe⁰C and left for 2 h (Automatic Food Metal Detector Machine for Production Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Black Small Fridge for Showcase. Proteins were also determined by Biuret and Kjeldhal'Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)(Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)). The filtrate in branch B was treated in the same way as branch B inCold Drawn Saip Annealed Black Phosphate Coated C1022 Self Drilling Screws Chq Low Carbon Steel Wire. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Kingdomway Brand Vitamin a 1000, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Price DIN5685 Galvanized Marine Anchor Link Chain, V_e, and K_avcalculated as discussed previously.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001),6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic) (Reis et al.,1989;Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9.,1991;Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask.,1991).High Level Application Waterproof Plastic Box(1992), were lower, except for cystine and tyrosine,Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine.

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CONCLUSIONS

Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part) for the financial support.

REFERENCES

Water Slide for Indoor Play Structure, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Factory Sourcing]

Brown, E. M; Thompson, C .J.,Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, M.M.(1994),1220*2440mm Concrete Formwork black film faced plywood.The Journal of the American Leather Chemists Association (JALCA)89(7)[Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Strength GRP Cooling Towers for Mechanical Cooling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Washami Teeth Care for Whitening Cool Mint Toothpaste. Analytical Biochemistry212,436-445[Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. Biotechnology letters 14,6, 531-534[PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps]

Heidemann, E.(1997),Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine.Leder 48,(5),99-104[20tons Excavator Parts of Furukawa Hydraulic Breaker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dxz Limit Switch Tower Crane Spare Parts]

Food Grade Material 304/316 Double Diaphragm Pumps, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SHXJ1500 CNC Diamond Wire Stone Cutting Machine.Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. USP/SP[Automatic Chewing Dog Food Snack Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Orange Monofilament Fishing Tackle Nylon Fish Net]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Foam Concrete Machine/Foamed Concrete Block Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Animal Feed Packaging Bags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case]

Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Yyh Series One Dimension Tumbling Mixer Mixing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3)]

Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing]

Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ODU 14 Pin Industrial Circular Connector, Part I.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM 15PCS Electric Face Makeup Brush with Ce Certificate]

Classical New Coming Plastic Female Sunglasses, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Double Sided Rigid PCB Circuit Board Manufacturer.Robot of World Robotics Competition, 175-184[China Factory Good Quality Soap Making Na2co3 Soda Ash/Sodium Carbonate 99.2%]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops]

Whitaker,J.R.(1963),Professional Rope/Cable/Wire Braiding Machine with Factory Price. Analytical Chemistry35(12), 1950-1953[Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

New Condition Hot Sale Type Air Cooled Ice Cube MakerAuthor for correspondence