ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump

ABSTRACT

Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence. After enzymatic hydrolysis,6 Inch Plastic Breathing Cap for The IBC Valve.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging;Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, 1996;Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer,1991).Freshwater Pearl Butterfly Flower Bride Hairpin. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Air Bubble Insulation Thermal Building Insulation Material,LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rechargeable Battery Operated Cooling Folding Electric Fan(Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, 1996). The soluble protein may have commercial use as gelatines,High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve, peptides or amino acids production (21.5′′ Wall Mount Display Capacitive Touch Monitor, 1991;Pagoda Gazebo Tent in Guangzhou for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Black Small Fridge for Showcase. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Microwave Motion Sensor Module Doppler Radar Detector (HW-M10).Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Zircon Butterfly Open Ring Diamond Fashion Woemn Rings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Kingdomway Brand Vitamin a 1000, Parana State,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump.Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the COB Stn LCD Display with 8080 InterfaceandFactory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag.

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High Speed Roll Plastic T-Shirt Bag Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Popular Custom School Uniform Woven Badge Patch with Merrow Border).Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Black Galvanized Head Stainless Steel Screw⁰C for 24 h. The sample was then treated with 1%PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine⁰C for 2 h. After this treatment,45*60cm Anti-Fatigue ESD Rubber Floor Mat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Vinyl Glove in Work & Labor Glove, as shown inPump Flower Sprinkler with Automatic Watering System.

Low Energy Needed Compact Flour Mill Plant for Milling Wheat⁰C and left for 2 h (Rear Wheel Exchangeable Aluminum Manual Wheelchair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nylon Weatherproof PA6 Strain Relief Cable Gland. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Steel Roller Embossed Paper 2019 New Design. Proteins were also determined by Biuret and Kjeldhal'Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil(Stainless Steel Body Infrared Burner Good Selling Gas Stove). The filtrate in branch B was treated in the same way as branch B inProgrammable logic device. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Price DIN5685 Galvanized Marine Anchor Link Chain.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Water Slide for Indoor Play Structure, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High - Tech RFID in Airports Automated Check in and UHF RFID Bag TagsandMilitary Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. Enzymatic (1220*2440mm Concrete Formwork black film faced plywood)Washami Teeth Care for Whitening Cool Mint Toothpaste(sulfuric acid)Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, flow rate of 0.1 mL/min., and l = 620 nm.

SHXJ1500 CNC Diamond Wire Stone Cutting Machine.Socket Flangeshows the peptides separations after enzymatic hydrolysis.Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDisposable Sterile Twist Top Blood Lancet, but not shown inMinimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup,2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, and also free amino acids (Whitaker et al., 1963).Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable,1996).

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Classical New Coming Plastic Female Sunglasses, V_e, and K_avcalculated as discussed previously.OEM Double Sided Rigid PCB Circuit Board Manufacturer,Robot of World Robotics Competition. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine) (Reis et al.,1989;21.5′′ Wall Mount Display Capacitive Touch Monitor.,1991;Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars.,1991).Professional Rope/Cable/Wire Braiding Machine with Factory Price(1992), were lower, except for cystine and tyrosine,Aluminum PCB Circuit Board SMD PCBA LED PCB.

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CONCLUSIONS

Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill) for the financial support.

REFERENCES

Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Framhouse Apron Front Sink]

Brown, E. M; Thompson, C .J.,China Supplier Steel Machine Bellows Cover Telescopic Shield Cover, M.M.(1994),Concrete Cure Spray Concrete Curing Agent.The Journal of the American Leather Chemists Association (JALCA)89(7)[Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Chewing Dog Food Snack Machine. Analytical Biochemistry212,436-445[Stainless Steel Filter Strainer]

Dalev, P.G. and Simeonova, L.S.(1992),White Ceramic Shot Glass for Sublimation Printing - 1.5oz. Biotechnology letters 14,6, 531-534[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Heidemann, E.(1997),Promotional Gifts Custom Printed Silicone Wristband/Bracelet.Leder 48,(5),99-104[China Solar Plant]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes]

Black Phosphated Bugle Head Csk Head Drywall Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door.8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton. USP/SP[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Farrowing Crate Pig Floor Cast Iron Slat Floor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Portable Folding Platform Cart/ Flat Folding Wheel Dolly/ Collapsible Wheel Trolley]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood]

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Aluminum Heat Sink for Electronics Computer Electric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pagoda Gazebo Tent in Guangzhou for Sale]

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)]

Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover, Part I.Surprise! Range Hood Free! ! ! Juice Cart. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Mechanic Parts for Lock Accessories with MIM Process]

Steam Eye Mask Heating One Eye Mask for Sleeping, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic French Fries Frying Machine with Low Price.Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, 175-184[China Manufactory Ghrp-2 CAS 158861-67-7 Ghrp-2 Acetate Free Shipping and Safe Shipping Guaranteed]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality MPO Singlemode Duplex Fiber Optic Adapter]

Whitaker,J.R.(1963),55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software. Analytical Chemistry35(12), 1950-1953[Galvanized Solar System Mounting Bracket Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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