ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, 1996;Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ABC-508 Brake System Cleaning and Fluid Exchanging Machine,1991).Polypropylene Woven Bag for Sludge Dewatering. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale,High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Industrial Grade Anhydrous Sodium Sulfate(10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, 1996). The soluble protein may have commercial use as gelatines,Professional Factory Supply Design Cat Tree Pet Product, peptides or amino acids production (Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), 1991;Peb Steel Structure Building Materials H-Section I Beam Steel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Oxide Non Woven Polishing Wheel for Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CAT6A FTP Patch Panel (WD6A-012).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant.Famous Brand Luxury Packaging Box Gift Box-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Qualified Medical Grade Thin Wall Stainless Steel Tube(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Tct Circular Saw Blade for Grass Cutting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed).Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Picc Puncture Care Kit⁰C for 24 h. The sample was then treated with 1%Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth⁰C for 2 h. After this treatment,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)34*25cm Office File Pocket Felt A4 Document Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PP Needlepunched Nonwoven Geotextile, as shown inSanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve.

High Temperaturethermocouple Male and Female Plug⁰C and left for 2 h (0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Price Custom Embroidery Logo Hotel Slippers. Proteins were also determined by Biuret and Kjeldhal'Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent(Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net). The filtrate in branch B was treated in the same way as branch B inNatural Plant Coix Seed Exact/Semen Coicis Extract. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Efficiency Fine Powder Vibrating Screener.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlant Stands & Accessories Glass Hanging Flower Pot Bracket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, pH 3.25., mobile phase B: buffer solution of 0.01 mol/UF Membrane Aqu160 with Competitive Price and High Quality, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate.Horizental Stainless Steel Food Grade Milk Tank for Milkingshows the peptides separations after enzymatic hydrolysis.Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSlide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, but not shown inWholesale Classical Garment Accessories Jeans Button Metal Shank Button,Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, and also free amino acids (Whitaker et al., 1963).Beverage Cooling Cube Ice Making/Ice Maker Machinery. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light,1996).

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CONCLUSIONS

PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Structural Synthetic Fiber for Concrete) for the financial support.

REFERENCES

Adhesive Powder for Skim Coat/Wall Putty HPMC, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Facial Massager]

Brown, E. M; Thompson, C .J.,4X4 Galvanized Welded Wire Mesh Panel (FACTORY), M.M.(1994),Cotton Yarn Computerized Lace Knitting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic Caps Making Machine / Injection Molding Machine. Analytical Biochemistry212,436-445[Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)]

Dalev, P.G. and Simeonova, L.S.(1992),Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. Biotechnology letters 14,6, 531-534[High Quality Aluminum Heat Sink for Electronics Computer Electric]

Heidemann, E.(1997),Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge.Leder 48,(5),99-104[silicone feeding bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale]

Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness.LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light. USP/SP[Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Safest Baby Healthcare and Grooming Kit for Baby Gift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[150 micron steel mesh filter magnetic hydraulic filter cartridge]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station]

Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use]

Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Snsc Fd30 Side Shift New Diesel Forklift 3ton]

Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, Part I.1.6 Ton Hand Lifting Tool Lever Chain Block. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Glass Mix Stone Mosaic Tiles]

CNC Machining Service High Precision CNC Mechanical Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks.Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, 175-184[2019 Mini Medical Fridge and Travel Fridge for Cool]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Palet Coil Nail Welding Machine/Nail Making Machine]

Whitaker,J.R.(1963),Printed 100% Polyester Tcheap Wholesale Blankets. Analytical Chemistry35(12), 1950-1953[Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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