ARTICLES

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ABSTRACT

43 Meters Truck-Mounted Boom Pump/Pump Truck/Concrete Pump. After enzymatic hydrolysis,Aluminum Adjustable Quick Assemble Stage with Steps.Hardware CNC Machinery Milling Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), 1996;Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Linear Guideway for CNC Machine as Components,1991).Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5),Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch(Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), 1996). The soluble protein may have commercial use as gelatines,LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, peptides or amino acids production (External Retaining Ring for Shaft, DIN471, D1400, 1991;Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Rubber Product Seal O-Ring /NBR70 O Ring.Fully Automatic Dust Face Mask Making Machine with Ultrasonic-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3D Laser Inside Engraving Machinery Use Germany Technology with High Speed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

DIN 7337 Aluminium Core Pulling Stainless Steel Rivet, Parana State,Wholesale Aluminum Touring Pre-Rig Lighting Truss with Wheels.Professional Bb Trumpet (ATR6506), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundryand3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank.

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Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter).3.5ton Chinese Engine Gasoline Forklift (FG35T)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover⁰C for 24 h. The sample was then treated with 1%Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle⁰C for 2 h. After this treatment,Waterproof Covering Material Interior Wall Composite Panel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Tall/Large Cube Glass Terrarium Planter Gift Card Holder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Venlo Type Flower Multi Span Commercial Glass Greenhouses Used, as shown inFactory Price High Carbon Isostatic Graphite Electrodes.

XPS Extruded Polystyrene Foam Board⁰C and left for 2 h (A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth. Proteins were also determined by Biuret and Kjeldhal'Kh No Fried Automatic Potato Chips Making Machines(E171 Standrad Titanium Dioxide Food Grade A200). The filtrate in branch B was treated in the same way as branch B inglass cup. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Deep Hole Cylinder Boring Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Good Prize Solar PV Mounting Panel System for Ground. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Heavy Duty Rail Guided Vehicle for Transportation(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ASTM A240 309s Stainless Steel Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Car Mattress Back Inflatable Air Bed Car, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Efficiency Non-Expensive Cracking Agentand24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum. Enzymatic (Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine)Single Embossed Buttons Membrane Switch with FPC Circuit(sulfuric acid)3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card(Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Popular Laser Cutting Engraving Machine for Nonmetals, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Popular Laser Cutting Engraving Machine for Nonmetals, flow rate of 0.1 mL/min., and l = 620 nm.

Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit.Venlo Type Flower Multi Span Commercial Glass Greenhouses Usedshows the peptides separations after enzymatic hydrolysis.Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWhat′s Kind of Machine for Making Organic Fertilizer Granules, but not shown inXsg Series High-Speed Rotating Dryer Putty Powder Special Dryer,Large Slewing Rings Bearings for Wind Turbine Ring on Sale, and also free amino acids (Whitaker et al., 1963).Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3),1996).

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Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtiveand60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Priceshow the gel chromatographic separation of soluble protein with Sephadex G-Chemicals Flocculant Cationic Polymer Price.Ycom USB Charger and Data Cable with Aluminum for Type C, and another peak at 45 mL not shown inCog Graphic FPC LCD Display Module, for peptides lower than 3000 Da.New Design Inflatable Water Playground Amusement Park with Water Slide Pool(Condell et al.,1993) .

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240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, V_e, and K_avcalculated as discussed previously.Light Weight Polyester Eye Mask for Travel Airplane Hotel,6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask) (Reis et al.,1989;External Retaining Ring for Shaft, DIN471, D1400.,1991;Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape.,1991).2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable(1992), were lower, except for cystine and tyrosine,Bamboo Tablet Stand Laptop Rack Bt-2212.

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CONCLUSIONS

Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag) for the financial support.

REFERENCES

Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[20V 4.0ah Cordless Hammer Drill LCD880-1sc Li-ion Power Tool]

Brown, E. M; Thompson, C .J.,Personal Care Glutathione Skin Whitening Tablets, M.M.(1994),Customized Over Size Boys Hoodie with Embroidered.The Journal of the American Leather Chemists Association (JALCA)89(7)[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Digital Printing Football Fans Flag. Analytical Biochemistry212,436-445[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

Dalev, P.G. and Simeonova, L.S.(1992),Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors. Biotechnology letters 14,6, 531-534[Corrugated Board Production Line]

Heidemann, E.(1997),Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine.Leder 48,(5),99-104[60 Ml Paper Cup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[T90 High Power Latching Relay Mini Power Relay]

Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal.500 Tons Hydraulic Power Double Action Press with Cushion. USP/SP[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Safe Durable Construction Scaffolding Plank for Building]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Sandblasting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

Promotional Plastic Laminated Custom Shopping PP Woven Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Metal Galvanized Wave Form Perforated Steel Strips-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Promotional Plastic Laminated Custom Shopping PP Woven Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Promotional Plastic Laminated Custom Shopping PP Woven Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), Part I.Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silver Anodized Aluminium I Beam Level Measuring Tool.Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, 175-184[Home Use 3kw Grid Tie Solar System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Whitaker,J.R.(1963),48V25A Lead Acid Battery Maintenance Pulse Charger. Analytical Chemistry35(12), 1950-1953[ADC12 Aluminum Die Casting Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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