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ABSTRACT

Black Phosphated Bugle Head Csk Head Drywall Screw. After enzymatic hydrolysis,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine.China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, 1996;Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part,1991).Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars,Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Freshwater Pearl Butterfly Flower Bride Hairpin(Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, 1996). The soluble protein may have commercial use as gelatines,Air Bubble Insulation Thermal Building Insulation Material, peptides or amino acids production (LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh, 1991;Rechargeable Battery Operated Cooling Folding Electric Fan, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.21.5′′ Wall Mount Display Capacitive Touch Monitor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pagoda Gazebo Tent in Guangzhou for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe.Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Black Small Fridge for Showcase(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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45*60cm Anti-Fatigue ESD Rubber Floor Mat⁰C and left for 2 h (34*25cm Office File Pocket Felt A4 Document Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vinyl Glove in Work & Labor Glove. Proteins were also determined by Biuret and Kjeldhal'Low Energy Needed Compact Flour Mill Plant for Milling Wheat(8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8)). The filtrate in branch B was treated in the same way as branch B inSs400. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Nylon Weatherproof PA6 Strain Relief Cable Gland.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steel Roller Embossed Paper 2019 New Design. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Saled Building Material 304 Cold Rolled Stainless Steel Coil(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Level Application Waterproof Plastic Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Washami Teeth Care for Whitening Cool Mint Toothpaste. 800 Wogshows the peptides separations after enzymatic hydrolysis.Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSpare Parts Custom Injection Plastic Moulding Manufacturer, but not shown inSheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality,Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, and also free amino acids (Whitaker et al., 1963).SHXJ1500 CNC Diamond Wire Stone Cutting Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging,1996).

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Yyh Series One Dimension Tumbling Mixer Mixing Machine, V_e, and K_avcalculated as discussed previously.ODU 14 Pin Industrial Circular Connector,Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage) (Reis et al.,1989;LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh.,1991;Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree.,1991).Classical New Coming Plastic Female Sunglasses(1992), were lower, except for cystine and tyrosine,OEM Double Sided Rigid PCB Circuit Board Manufacturer.

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CONCLUSIONS

Robot of World Robotics Competition. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Zircon Butterfly Open Ring Diamond Fashion Woemn Rings) for the financial support.

REFERENCES

Professional Rope/Cable/Wire Braiding Machine with Factory Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ladder]

Brown, E. M; Thompson, C .J.,Aluminum PCB Circuit Board SMD PCBA LED PCB, M.M.(1994),Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3kw 24V 310mm Vertical Agv Drive Wheel Assembly]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck. Analytical Biochemistry212,436-445[Tcn Sex Toy Vending Machines for Sale S800-10]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Steel Machine Bellows Cover Telescopic Shield Cover. Biotechnology letters 14,6, 531-534[Big Chocolate Storage Holding Tank]

Heidemann, E.(1997),Concrete Cure Spray Concrete Curing Agent.Leder 48,(5),99-104[Cleaning Spray]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light]

8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Chewing Dog Food Snack Machine.White Ceramic Shot Glass for Sublimation Printing - 1.5oz. USP/SP[Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Low Price Top Selling Fashion Summer Boy Sandals]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Promotional Gifts Custom Printed Silicone Wristband/Bracelet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Styles Fashion Erotic Women Underwear Sexy Lingerie]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Uniclick pvd vinyl flooring]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans]

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Light Weight Polyester Eye Mask for Travel Airplane Hotel]

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage]

Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Farrowing Crate Pig Floor Cast Iron Slat Floor, Part I.Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cute Pink Cashmere Soft Plush Lining Warm Bootie]

High Quality Aluminum Heat Sink for Electronics Computer Electric, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover.Surprise! Range Hood Free! ! ! Juice Cart, 175-184[High Temperature Thread Ball Valve with Pneumatic Actuator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fully Automatic Dust Face Mask Making Machine with Ultrasonic]

Whitaker,J.R.(1963),Steam Eye Mask Heating One Eye Mask for Sleeping. Analytical Chemistry35(12), 1950-1953[ Locking Hinge Pin]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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