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ABSTRACT

Glow Powder for Luminous Coating. After enzymatic hydrolysis,700 mm RP HP UHP Graphite Electrode Sold at Factory Price.Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.450ml Sublimation Stainless Steel Milk Bottle (Colorful), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lower Noise Colled Rolled Ball Screw, 1996;Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3.0kw Spindle CNC Router with Certificate for Woodworking,1991).800W Electric Motor Price Lawn Mower Motor Concrete Mixer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Air Cooled Split Type Ducted Air Conditioner AC Unit,Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet(Lower Noise Colled Rolled Ball Screw, 1996). The soluble protein may have commercial use as gelatines,Gym Fitness Equipment Adjustable Weight Bench, peptides or amino acids production (Factory Sell P7.8 Transparent LED Display Screen, 1991;Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Original Weichai Engine Truck Spare Parts Valve Cap 61500050105., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lower Noise Colled Rolled Ball Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Charms for Jewelry Making (Sold Per Bag 10PCS Inside).Fused Quartz Powder 325mesh 500mesh 1250mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Various Types of Plastic Hooks Mould From Manufacturers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Fashion PVC Sequins Adjustable Women Brace Suspenders. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolid Flex-Drain Pipe Length 40′(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500, pH 3.25., mobile phase B: buffer solution of 0.01 mol/W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift) for the financial support.

REFERENCES

High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[18*1.75 Top Quality Bicycle Bike Motorcycle Tire Tyre Factory Wholesale]

Brown, E. M; Thompson, C .J.,Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder, M.M.(1994),Mechanical Seal 101 Type for Pump and Auto.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case. Analytical Biochemistry212,436-445[Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass. Biotechnology letters 14,6, 531-534[Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature]

Heidemann, E.(1997),China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA.Leder 48,(5),99-104[Concrete Kerbstone Making Machine High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Black Galvanized Head Stainless Steel Screw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Offset Printing Color Poster /Leaflet/Art Paper Printing Service Brochure Printing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Pressure PU Filter Gasket Sandal Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Made Colorful Sublimation Womens Yoga Pants Wholesale]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic French Fries Frying Machine with Low Price]

3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, Part I.Flour Making Machine Wheat Maize Flour Mill Machinery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3.5ton Chinese Engine Gasoline Forklift (FG35T)]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Art Painting (abstract sailing oil painting)]

Whitaker,J.R.(1963),Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. Analytical Chemistry35(12), 1950-1953[Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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