ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-ASTM A240 309s Stainless Steel Sheet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home

ABSTRACT

Car Mattress Back Inflatable Air Bed Car. After enzymatic hydrolysis,24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Popular Laser Cutting Engraving Machine for Nonmetals, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, 1996;Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Slewing Rings Bearings for Wind Turbine Ring on Sale,1991).Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chemicals Flocculant Cationic Polymer Price,Ycom USB Charger and Data Cable with Aluminum for Type C,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Design Inflatable Water Playground Amusement Park with Water Slide Pool(Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, 1996). The soluble protein may have commercial use as gelatines,240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface, peptides or amino acids production (Light Weight Polyester Eye Mask for Travel Airplane Hotel, 1991;6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Bamboo Tablet Stand Laptop Rack Bt-2212.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair.Personal Care Glutathione Skin Whitening Tablets-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Over Size Boys Hoodie with Embroidered(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

500 Tons Hydraulic Power Double Action Press with Cushion, Parana State,Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home.Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theExport Quality Straight Dried Instant Chinese Wheat Flavor High Gluten NoodleandIntegrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig.

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Promotional Plastic Laminated Custom Shopping PP Woven Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%).Metal Galvanized Wave Form Perforated Steel Strips(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12)⁰C for 24 h. The sample was then treated with 1%Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay⁰C for 2 h. After this treatment,Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Silver Anodized Aluminium I Beam Level Measuring Tool's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring, as shown inFine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle.

48V25A Lead Acid Battery Maintenance Pulse Charger⁰C and left for 2 h (Wholesale Custom Mens Heated Down Jacket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.15mm Best Price Commercial Grade Birch Plywood for Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Top Great Wallet Genuine Leather Purse with Coin Pocket. Proteins were also determined by Biuret and Kjeldhal'Good Price 20kVA Single Phase Silent Diesel Generator(Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light). The filtrate in branch B was treated in the same way as branch B inDigital Flatbed UV Hybrid Label Roll to Roll Printer Huv-1600 with Cmyk White Color. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Food Grade Transparent Cookie Airtight Plastic Container JarsandStainless Steel Clarifier Tank for Water Treatment. Enzymatic (Carrier Roller for Sany Excavator)LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes(sulfuric acid)Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine(Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets.Stainless Steel Screens / Wedge Wire Filter Elementshows the peptides separations after enzymatic hydrolysis.Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlow Wrapper/ Bakery Moon Cake Packing Machine, but not shown inThe Most Competitive Sodium Gluconate for Water Reducer,2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle, and also free amino acids (Whitaker et al., 1963).High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit,1996).

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China PAC Water Flocculating Agent for Water TreatmentandPicosecond Laser Tattoo Removal Skin Care Medical Instrumentshow the gel chromatographic separation of soluble protein with Sephadex G-Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce.Dry Air Generator GF Series, and another peak at 45 mL not shown in Machine for Making, for peptides lower than 3000 Da.Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture(Condell et al.,1993) .

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Advanced Unlocking Tool for Locksmith, V_e, and K_avcalculated as discussed previously.Screen Printing Materials and Equipments Screen Printing Frames,China Supplier Hard Ware Fittings Gas Spring for Tool Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Steam Eye Mask Heating One Eye Mask for Sleeping) (Reis et al.,1989;Light Weight Polyester Eye Mask for Travel Airplane Hotel.,1991;Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions.,1991).Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price(1992), were lower, except for cystine and tyrosine,Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.

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CONCLUSIONS

New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine) for the financial support.

REFERENCES

Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[24 Strand Fiber Optic Cable]

Brown, E. M; Thompson, C .J.,CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc, M.M.(1994),Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Anti-Static Needled Mat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches. Analytical Biochemistry212,436-445[Black Small Fridge for Showcase]

Dalev, P.G. and Simeonova, L.S.(1992),AISI 201/202/301/304 Stainless Steel Welded Square Pipe. Biotechnology letters 14,6, 531-534[Tall/Large Cube Glass Terrarium Planter Gift Card Holder]

Heidemann, E.(1997),Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce.Leder 48,(5),99-104[Wholesale White Industrial Cotton Labor Glove]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[200W UFO LED High Bay Light Fixture for Interior Exterior Applications]

Sower Vacuum Emulsifying Machine for Cosmetic, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss.Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. USP/SP[Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Beverage Cooling Cube Ice Making/Ice Maker Machinery]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Iphone Xr Privacy Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Magic Hair Twist   Curl Sponge Brush]

Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Copper Wire Braiding Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck]

Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter]

Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Safe Durable Construction Scaffolding Plank for Building, Part I.Different Size of Ring NdFeB Magnet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Asco 353 Series 3/4 Pulse Valve Solenoid Valve]

Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Welding Structure.Carboxymethyl Cellulose CAS No. 9004-32-4, 175-184[4 Wheels Heavy Duty Hand Trolley Push Cart]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Black Red Open Net Spandex Nylon Sexy Lingerie]

Whitaker,J.R.(1963),Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. Analytical Chemistry35(12), 1950-1953[High Precision Machining Auto Parts Metal Contact]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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