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ABSTRACT

6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck. After enzymatic hydrolysis,High Level Application Waterproof Plastic Box.Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1220*2440mm Concrete Formwork black film faced plywood, 1996;Washami Teeth Care for Whitening Cool Mint Toothpaste., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope,1991).Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SHXJ1500 CNC Diamond Wire Stone Cutting Machine,Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 Foam Concrete Machine/Foamed Concrete Block Making Machine(1220*2440mm Concrete Formwork black film faced plywood, 1996). The soluble protein may have commercial use as gelatines,Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, peptides or amino acids production (Yyh Series One Dimension Tumbling Mixer Mixing Machine, 1991;ODU 14 Pin Industrial Circular Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Classical New Coming Plastic Female Sunglasses.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Double Sided Rigid PCB Circuit Board Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1220*2440mm Concrete Formwork black film faced plywood, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Robot of World Robotics Competition.Professional Rope/Cable/Wire Braiding Machine with Factory Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum PCB Circuit Board SMD PCBA LED PCB(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Promotional Gifts Custom Printed Silicone Wristband/Bracelet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3HP Industrial Cooling System Water Cooled Chiller).Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton⁰C for 24 h. The sample was then treated with 1%Farrowing Crate Pig Floor Cast Iron Slat Floor⁰C for 2 h. After this treatment,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Aluminum Heat Sink for Electronics Computer Electric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover, as shown in355nm UV Laser Marking Equipment for Crystal Materials.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNatural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/T90 High Power Latching Relay Mini Power Relay, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic Caps Making Machine / Injection Molding MachineandAuto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430. Enzymatic (Polyglycolic Acid Absorable Suture)No. 1062 20PCS Tools(sulfuric acid)Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant(1220*2440mm Concrete Formwork black film faced plywood, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluminum Mould Shoe Mould Making CNC Milling Router Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluminum Mould Shoe Mould Making CNC Milling Router Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Supplier Steel Machine Bellows Cover Telescopic Shield Cover) for the financial support.

REFERENCES

Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pc750-7 Hydraulic Pump]

Brown, E. M; Thompson, C .J.,Single Stage High Speed Fruit and Vegetable Pulping Machine, M.M.(1994),China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. Analytical Biochemistry212,436-445[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

Dalev, P.G. and Simeonova, L.S.(1992),Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads. Biotechnology letters 14,6, 531-534[Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service]

Heidemann, E.(1997),Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard.Leder 48,(5),99-104[Drilling Screw With Rubber Washer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Air Cooled Split Type Ducted Air Conditioner AC Unit]

Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Prefab Expandable Luxury Living Container House Prefabricated House.Movable Partition Walls for Room Division, Hotel and Shopping Mall. USP/SP[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Made in China Lqa Strap Banding Tool]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Carboxy Terminal Collagen Crosslinks Elisa Kits: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality PU/ Micro Fiber Leather Fighting Gloves]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Modern Pharmacy Vacuum Filter Press]

Various Colors Nylon and Stainless Steel Kitchen Tools, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),355nm UV Laser Marking Equipment for Crystal Materials-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Folding Play Mat Crawl Play Mat]

Various Colors Nylon and Stainless Steel Kitchen Tools,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dbry-320 Christmas Stickers Labels Printing Machine]

Various Colors Nylon and Stainless Steel Kitchen Tools, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Metal Separating Gold Brass Zippers for Sale, Part I.Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Density Celuca Kitchen Cabinets PVC Foam Board]

OEM Refrigeration Digital Thermometer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L).Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, 175-184[Disposable Pedicure Manicure Set Customized Logo]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fiber Optic Sc APC Fast Connector for FTTH Drop Cable]

Whitaker,J.R.(1963),79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar. Analytical Chemistry35(12), 1950-1953[Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Foil PE Bottle Cap Induction Seal LinerAuthor for correspondence