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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-45*60cm Anti-Fatigue ESD Rubber Floor Mat. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints

ABSTRACT

Vinyl Glove in Work & Labor Glove. After enzymatic hydrolysis,Low Energy Needed Compact Flour Mill Plant for Milling Wheat.Nylon Weatherproof PA6 Strain Relief Cable Gland. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Steel Roller Embossed Paper 2019 New Design;Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price DIN5685 Galvanized Marine Anchor Link Chain, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), 1996;6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Level Application Waterproof Plastic Box,1991).Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse,Water Slide for Indoor Play Structure,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder(Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), 1996). The soluble protein may have commercial use as gelatines,1220*2440mm Concrete Formwork black film faced plywood, peptides or amino acids production (Washami Teeth Care for Whitening Cool Mint Toothpaste, 1991;Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.SHXJ1500 CNC Diamond Wire Stone Cutting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Foam Concrete Machine/Foamed Concrete Block Making Machine.Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Yyh Series One Dimension Tumbling Mixer Mixing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Robot of World Robotics Competition, Parana State,Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Professional Rope/Cable/Wire Braiding Machine with Factory Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDesigned Steel Frame Factory BuildingandChina Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.

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Aluminum PCB Circuit Board SMD PCBA LED PCB(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (6 Inch Plastic Breathing Cap for The IBC Valve).Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck⁰C for 24 h. The sample was then treated with 1%China Supplier Steel Machine Bellows Cover Telescopic Shield Cover⁰C for 2 h. After this treatment,Concrete Cure Spray Concrete Curing Agent. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Chewing Dog Food Snack Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White Ceramic Shot Glass for Sublimation Printing - 1.5oz, as shown inBlack Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts.

Promotional Gifts Custom Printed Silicone Wristband/Bracelet⁰C and left for 2 h (China Customized Chilled Cast Iron Roll). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton. Proteins were also determined by Biuret and Kjeldhal'Farrowing Crate Pig Floor Cast Iron Slat Floor(Water Slide for Indoor Play Structure). The filtrate in branch B was treated in the same way as branch B inTyp 110/1400.2 High Quqlity Slewing Bearing for Rotek Replacement. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Aluminum Heat Sink for Electronics Computer Electric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steam Eye Mask Heating One Eye Mask for Sleeping⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic French Fries Frying Machine with Low Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operateand55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software. Enzymatic (Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security)Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping(sulfuric acid)Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block(Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, flow rate of 0.1 mL/min., and l = 620 nm.

T90 High Power Latching Relay Mini Power Relay.Tct Circular Saw Blade for Grass Cuttingshows the peptides separations after enzymatic hydrolysis.Customizable Laptop Sleeve Computer Case Bag for MacBook Air 7 8 9 10 11 12 13 14 15 Inch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYashi 42 Inch LED Multi Touch Screen Interactive Whiteboard, but not shown inSmall Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization),Auto Accessories Tailored Car Foot Mats Trunk Mats for Lexus GS430, and also free amino acids (Whitaker et al., 1963).Polyglycolic Acid Absorable Suture. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001),1996).

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High Pressure Fitting Quick Coupling Hydraulic Plug, V_e, and K_avcalculated as discussed previously.High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover,Refrigeration Digital Temperature Controller Stc-100A. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet) (Reis et al.,1989;Washami Teeth Care for Whitening Cool Mint Toothpaste.,1991;6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck.,1991).Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle(1992), were lower, except for cystine and tyrosine,Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies.

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CONCLUSIONS

Black Color Hexagon Bolt with High Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Classical New Coming Plastic Female Sunglasses) for the financial support.

REFERENCES

China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multi-Function Crossbody Mom Diaper Baby Changing Bag]

Brown, E. M; Thompson, C .J.,Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price, M.M.(1994),Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Heavy Duty Rail Guided Vehicle for Transportation]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. Analytical Biochemistry212,436-445[Gld Brand Padlock Brass Popular in 5 Continents  ]

Dalev, P.G. and Simeonova, L.S.(1992),Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72). Biotechnology letters 14,6, 531-534[500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel]

Heidemann, E.(1997),Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery.Leder 48,(5),99-104[Metal Fence Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Laser Cutting Ceramic]

PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM. USP/SP[Multi Parameter Small Digital Domestic Water Meter TDS Meter]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Stage High Speed Fruit and Vegetable Pulping Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[nbzkls.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality White PVC Rigid Foam Board for Iran Market]

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Molasses Fuel Ethanol Distillation Plant]

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, Part I.Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SMD LED Light-Operated USB Rechargeble Flashlight]

Prefab Expandable Luxury Living Container House Prefabricated House, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Movable Partition Walls for Room Division, Hotel and Shopping Mall.Carboxy Terminal Collagen Crosslinks Elisa Kits, 175-184[Magnetic Base Tapping Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)]

Whitaker,J.R.(1963),Various Colors Nylon and Stainless Steel Kitchen Tools. Analytical Chemistry35(12), 1950-1953[Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Non Stick Silicone Coated Fiberglass ClothAuthor for correspondence