ARTICLES

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ABSTRACT

Hanwha SMT Chip Shooter LED Pick and Place Machine. After enzymatic hydrolysis,ACP Aluminium Cmposite Panel Cladding Extrusions.New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Wholesale Custom Mens Heated Down Jacket;Rectification Triming Metallized Polyester Film Slitting Rewinding Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Swim Shorts Grey Mens Board Shorts with Mesh Liner, 1996;Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization),1991).Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Marine Mooring Hardware, Anchor Chain,Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick(New Swim Shorts Grey Mens Board Shorts with Mesh Liner, 1996). The soluble protein may have commercial use as gelatines,QJ-M Moving small manual brick making machine with low cost, peptides or amino acids production (Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 1991;Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Braiding Machine for Weaving Flexible Metal Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Metal Button Crystal Shank Button for Garment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Swim Shorts Grey Mens Board Shorts with Mesh Liner, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616).10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Industrial Grade Anhydrous Sodium Sulfate, Parana State,High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt.Professional Factory Supply Design Cat Tree Pet Product, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Swim Shorts Grey Mens Board Shorts with Mesh Liner, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDark Blue Color Texture Indoor Swimming Pool Glass Mosaic TileandPlanter Moving Round Planter Saucer Casters Plant Trolley (M).

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Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel).Peb Steel Structure Building Materials H-Section I Beam Steel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Oxide Non Woven Polishing Wheel for Machine⁰C for 24 h. The sample was then treated with 1%CAT6A FTP Patch Panel (WD6A-012)⁰C for 2 h. After this treatment,LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Famous Brand Luxury Packaging Box Gift Box, as shown inLED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.

High Qualified Medical Grade Thin Wall Stainless Steel Tube⁰C and left for 2 h (ABC-508 Brake System Cleaning and Fluid Exchanging Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Strength New 0.18mm Molybdenum Wire. Proteins were also determined by Biuret and Kjeldhal'Structural Synthetic Fiber for Concrete(Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)). The filtrate in branch B was treated in the same way as branch B inm.cnsdhead.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Picc Puncture Care Kit, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Price Custom Embroidery Logo Hotel Slippers.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronateshows the peptides separations after enzymatic hydrolysis.Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, but not shown inMetal Separating Gold Brass Zippers for Sale,High Efficiency Fine Powder Vibrating Screener, and also free amino acids (Whitaker et al., 1963).High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Swim Shorts Grey Mens Board Shorts with Mesh Liner,1996).

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CONCLUSIONS

High Precision C45 Steel CNC Lathe Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale) for the financial support.

REFERENCES

I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Construction Building]

Brown, E. M; Thompson, C .J.,CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate, M.M.(1994),Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flat Pack Temperary Container House 20FT 40FT]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Grade PTFE Coated Fiberglass Mesh Baking Sheet. Analytical Biochemistry212,436-445[High Quality Engieered Veneer Faced Commericial Plywood]

Dalev, P.G. and Simeonova, L.S.(1992),Beverage Cooling Cube Ice Making/Ice Maker Machinery. Biotechnology letters 14,6, 531-534[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

Heidemann, E.(1997),Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank.Leder 48,(5),99-104[Folding Kick Electric Scooter for Adult and Kids]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Transparent Composite Fiberglass Roof Tiles.Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. USP/SP[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Positive Drive Turn Belt for Spiral Freezing Equipment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Two Layer License Plates with Wide Border Embossed: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yitai Die Making 13mm Beech Rotary Plywood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Used Cranes In Dubai]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Really Microfiber PVC Leather for Sofa Furniture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle]

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51]

Electrical Barber Shop Equipment Electric Hair Curling Wand Iron, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:94V0 Circuit Board Mobile Phone PCB Motherboard, Part I.Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels]

PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Adhesive Powder for Skim Coat/Wall Putty HPMC.4X4 Galvanized Welded Wire Mesh Panel (FACTORY), 175-184[Annulus-Microfracture Gas-Migration Products Made in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope]

Whitaker,J.R.(1963),Cotton Yarn Computerized Lace Knitting Machine. Analytical Chemistry35(12), 1950-1953[Australia C-Tick Testing and Certification Service for Electric Scooter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Carbon Steel Manifold Aluminum Tee Pipe Welding Transition JointsAuthor for correspondence