ARTICLES

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ABSTRACT

Loquat Juice and Loquat Syrup Production Machinery Export Worldwide. After enzymatic hydrolysis,PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material.Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Concrete Floor Grinding Disc Machine for Four Segments;Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Garden Furniture Rattan Sofa Set with Ottomen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine, 1996;Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Non Standard U Channel Steel,1991).Socket Flange. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Dn32 (11/4) BSPT Thread Air Actuated Piston Valves,Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine(Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine, 1996). The soluble protein may have commercial use as gelatines,Disposable Sterile Twist Top Blood Lancet, peptides or amino acids production (Industrial Control Housing with Plate Shape (XD-CC04), 1991;Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC 3 Axis Milling Machining Center. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting.Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4 Axis CNC Woodworking Machinery Engraver Router(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0Alcalase2.5 L DX,Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Pre-Seasoned Cast Iron Baking Stone Cookware(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Compression Polyester Light Ankle Support Brace for Youth, Parana State,Custom Made Fabrication Service Anodized Aluminium CNC Machining Parts.Good Quality White PVC Rigid Foam Board for Iran Market, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSanhe FC-2 Poultry Air VentandChina OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.

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Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Kh No Fried Automatic Potato Chips Making Machines).Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Precision Piston Ring Good Shape Ability High Quality Surface⁰C for 24 h. The sample was then treated with 1%Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002⁰C for 2 h. After this treatment,Farm Relief Plastic Automatic Air Release Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Plastic ABS/PMMA/PC/PP/as Material Components Injection Mold for Industry, as shown inSolar Energy Storad Power Generation System.

Square Decorative Stainless Steel Crimped Wire Mesh⁰C and left for 2 h (PP Needlepunched Nonwoven Geotextile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mold Injection Molding Plastic Products White PC Housing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sw Type BOPP Packaging Adhesive Tape Coating Machine. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Metric Female Flat Seat Hose Fittings(6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring). The filtrate in branch B was treated in the same way as branch B inShrink Tunnel for Packing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMulti Color Spade Made of Steel and Panting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Condition Hot Sale Type Air Cooled Ice Cube Maker, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Hinge Pin, Locking Hinge Pin, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling.Electrical Stationary Hoist Tower Crane 500kg Motorshows the peptides separations after enzymatic hydrolysis.Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton, but not shown inFM USB Radio Audio Power Amplifier for Home Bar Club,100W 120W 150W Outdoor Solar LED Street Lighting, and also free amino acids (Whitaker et al., 1963).Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine,1996).

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CONCLUSIONS

Varied Colored As568 Food Grade Soft Rubber Silicone O Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216) for the financial support.

REFERENCES

2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Heat Press Machine Small]

Brown, E. M; Thompson, C .J.,Chinese Galvanized Rectangular Tube for Buildings Materials, M.M.(1994),China Customized Chilled Cast Iron Roll.The Journal of the American Leather Chemists Association (JALCA)89(7)[Slewing Gear for Tower Crane Slewing Winch Reducer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IMTEK High Precision and Rigidity Linear Guides for CNC machinery. Analytical Biochemistry212,436-445[Mechanical Seal 101 Type for Pump and Auto]

Dalev, P.G. and Simeonova, L.S.(1992),Steam Sleeping Eye Mask, Heated Eye Mask, Eye Steam Mask. Biotechnology letters 14,6, 531-534[Outdoor Garden Save Energy with Solar Flame Wall Light]

Heidemann, E.(1997),Yashi Touch Screen Smart Interactive Whiteboard for Office.Leder 48,(5),99-104[Plastic Composite Asa Roof Tile]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder]

Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electric Fireplace PCBA with Double Layers Circuit Board.China Shipping Logistics to Stockholm. USP/SP[Multifunctional Solar Powered Safety Hammer LED Flashlight]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jk Long Rope Small Electric Winch 5ton for Pulling and Lifting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Shell and Tube Heat Exchanger Used for Air Compressor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Steel Forged Automobile Spare Parts with Customized Drawing]

Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Animal Agricultural Damping Horse Stall Rubber Cow Mat, Part I.CNC Automatic Laser Wood Cutting Machine Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine]

Yc Pure White Nylon Powder for 3D Printing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats.Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM, 175-184[Q235 Grade and 0.12mm-1.2mm Thickness PPGI/Corrugated Zinc Roofing Sheet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 100p]

Whitaker,J.R.(1963),High Quality Liquid Spouted Stand up Pouch with Custom Logo. Analytical Chemistry35(12), 1950-1953[Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chinese Knuckle Boom Hydraulic Truck Mounted Crane for SaleAuthor for correspondence