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ABSTRACT

Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. After enzymatic hydrolysis,Groove Type Wood Log Tree Peeling and Debarking Machine.High Quality CE Digital Display Adjustable Power Supply. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Non-Expensive Cracking Agent, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, 1996;Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Striped Compression Soccer Socks for Adults,1991).Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen,High Tensile Strength Galvanized Steel Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,24 Volt Fire Alarm Strobe Light Horn Strobe Sounder(Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, 1996). The soluble protein may have commercial use as gelatines,from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder, peptides or amino acids production (Best Price PVC Coated Fiberglass Mesh Insect Window Screen, 1991;Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory OEM CNC Machined Gear Motor Shaft.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pink French Loop Terry Knit Fabric for Jacket.Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cute Pink Cashmere Soft Plush Lining Warm Bootie(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage).3kw 24V 310mm Vertical Agv Drive Wheel Assembly(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sofa Materials PU Leather with Flock Backing⁰C for 24 h. The sample was then treated with 1%Three Phase 800mm Plate Fan⁰C for 2 h. After this treatment,ASTM Approved Popular Indoor Trampoline of Park. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light, as shown inCute Pink Cashmere Soft Plush Lining Warm Bootie.

Wholesale Genuine Leather Credit Card Mens Wallet⁰C and left for 2 h (Fully Automatic Dust Face Mask Making Machine with Ultrasonic). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yitai Die Making 13mm Beech Rotary Plywood. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gear Rolling Machine Zh28/25 Spline Hobbing Machine. Proteins were also determined by Biuret and Kjeldhal'OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts( Locking Hinge Pin). The filtrate in branch B was treated in the same way as branch B inCNC Turning Micro 6061 Aluminum Parts OEM Machining. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Partial Thread Steel/Iron Hex Head Self Tapping Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlat Car Sun Roof Tent Awning(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pneumatic Control Shuttle Valve NPT Thread, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized Marathon Medal for Marathon MedallionandFour Column Hydraulic Press Machine for Kitchen Sink Deep Drawing. Enzymatic (OEM 15PCS Electric Face Makeup Brush with Ce Certificate)New Styles Fashion Erotic Women Underwear Sexy Lingerie(sulfuric acid)on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale(Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stand Hot and Cold Normal Water Dispenser with Refrigerator, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stand Hot and Cold Normal Water Dispenser with Refrigerator, flow rate of 0.1 mL/min., and l = 620 nm.

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Dbry-320 Christmas Stickers Labels Printing Machine, V_e, and K_avcalculated as discussed previously.Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor,Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles) (Reis et al.,1989;Best Price PVC Coated Fiberglass Mesh Insect Window Screen.,1991;Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch.,1991).14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna(1992), were lower, except for cystine and tyrosine,Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad.

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CONCLUSIONS

Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)) for the financial support.

REFERENCES

Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Non-Woven Fabric Glove Wipes Making Machine]

Brown, E. M; Thompson, C .J.,High Cut Genuine Leather Safety Shoes for Electrician in Guangzhou, M.M.(1994),High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Turning Center]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Popular Industrial Sewing Quilting Machine With CO]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminum Bright Polished Mirror Sheet. Analytical Biochemistry212,436-445[Stainless Steel Seamless Pipe ASTM A312 TP304 316]

Dalev, P.G. and Simeonova, L.S.(1992),Chik Chrome Steel High Carbon Ball Bearing for Motor Bike Automobile (6215 RS ZZ open). Biotechnology letters 14,6, 531-534[Concrete Cure Spray Concrete Curing Agent]

Heidemann, E.(1997),Professional Motorcycle Reflector Injection Plastic Parts Mould Maker.Leder 48,(5),99-104[Bouillon Cube Tablet Press Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet and Animal Food Extrusion Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Level Application Waterproof Plastic Box]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiberglass Asphalt/Bitumen Roof Shingles for Cement and Wooden Roof]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker]

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Customized Souvenirs Tin Metal Number & Name Plate for Car,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Biogas/Methane Purification System/Desulfurization/Scrubber Tower]

Customized Souvenirs Tin Metal Number & Name Plate for Car, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Molasses Fuel Ethanol Distillation Plant, Part I.Pond Self Priming Booster Pressure Water Pump with Check Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Pressure Locked Grating]

Banded Type Tee Used on Oil, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),20FT New Shipping Container 40FT Dry Cargo Container.High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, 175-184[Carbon Laminated Scrims Fabric For Flooring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine]

Whitaker,J.R.(1963),Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone. Analytical Chemistry35(12), 1950-1953[Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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