ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Pressure Braided Rubber Oil Hose. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Monochrome Graphic Industrial Control LCD Display Module 128X64

ABSTRACT

SMD LED Light-Operated USB Rechargeble Flashlight. After enzymatic hydrolysis,Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden.1jo4 Hydraulic Adaptors for Hose Fittings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chinese 1325 Ce Certificated CNC Engraving Router for Sale;Oil Heating Reactor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996;All Kinds of Color Fashion Simple Stone Pendant Design Necklace., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve,1991).48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb,Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Toilet Roll Tissue Wrapping Machine with Auto - Counting(6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996). The soluble protein may have commercial use as gelatines,Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine, peptides or amino acids production (Orange Monofilament Fishing Tackle Nylon Fish Net, 1991;Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;By6-8-200-L Automatic Doypack Premade Pouch Packing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Diameter 1300mm Lifting Electro Magnet for Cast Iron.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Evaporator Finned Tube.Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Vitamin D3 Fat-Solubility Water-Solubility Feed Additive(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Titanium Dioxide Anatase for Paper Making).Factory Wholesale Zinc Plated Carbon Steel Hex Nut(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern⁰C for 24 h. The sample was then treated with 1%Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V⁰C for 2 h. After this treatment,Stainless Steel Cheese Making Machine Electric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100W Dimmable LED Corn Bulb with Meanwell Driver, as shown inPTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal.

Pet and Animal Food Extrusion Machine⁰C and left for 2 h (Aluminum Mould Shoe Mould Making CNC Milling Router Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hexagonal Wire Mesh Chicken Wire Mesh High Quality. Proteins were also determined by Biuret and Kjeldhal'V-32 New Special UPS Solar Battery Cabinet Box Case(10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light). The filtrate in branch B was treated in the same way as branch B in13/15/20mm Aluminum Crimp Perfume Pump Airless  Pump  Bottles Hot Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4-14 Manual Staple Gun with Staples for Hand Tools.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Jewelry Lab Gemstone 925 Sterling Silver Pendant Necklace with Garnet/Citrine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Biomass Drum Wood Chipper with Competitive Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Forged Steel Pressure Sealed Bonnet Globe Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale Safety Vinyl PVC Flooring.Sower Vacuum Emulsifying Machine for Cosmeticshows the peptides separations after enzymatic hydrolysis.Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEfficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability, but not shown inBest Quality Bending Metal Mould with All Kinds Metal Materials,Horizental Stainless Steel Food Grade Milk Tank for Milking, and also free amino acids (Whitaker et al., 1963).Multi Parameter Small Digital Domestic Water Meter TDS Meter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring,1996).

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CONCLUSIONS

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Turning Center, CNC Horizontal Turning Milling Machine) for the financial support.

REFERENCES

Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Glass Oil Mister]

Brown, E. M; Thompson, C .J.,Explosion-Proof Housing Sealing Dispensing Machine, M.M.(1994),Tube Mills for Thin Wall Tubing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rechargeable Battery Operated Cooling Folding Electric Fan]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. Analytical Biochemistry212,436-445[Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop]

Dalev, P.G. and Simeonova, L.S.(1992),Car Badge Custom Emblem Auto Logo Sign for Advertising. Biotechnology letters 14,6, 531-534[Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly]

Heidemann, E.(1997),2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.Leder 48,(5),99-104[China Supplier Cold Room]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sw Type BOPP Packaging Adhesive Tape Coating Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rear Wheel Exchangeable Aluminum Manual Wheelchair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12 Bottles Portable Home Ues Thermoelectric Wine Chiller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Feso4.7H2O Water Treatment Ferrous Sulfate Heptahydrate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fused Quartz Powder 325mesh 500mesh 1250mesh]

90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Graphic 128X128 Dots Cog LCD-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)]

90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16]

90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, Part I.Tcn Sex Toy Vending Machines for Sale S800-10. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ X Ray Room Door]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1]

Whitaker,J.R.(1963),Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler. Analytical Chemistry35(12), 1950-1953[Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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