ARTICLES

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ABSTRACT

DC 12V Power Relay for Smart House. After enzymatic hydrolysis,2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment.Medical Human Body Thermometer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.;Greenhouse Fixing Film Profile Lock Channel and Spring Wire; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996;RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket,1991).Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52,China Supplier Granular Coal Based Activated Carbon Filter Meida,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Glass Cutting Table/CNC Glass Cutting Machine(Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996). The soluble protein may have commercial use as gelatines,12 Bottles Portable Home Ues Thermoelectric Wine Chiller, peptides or amino acids production (Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, 1991;Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe.Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Custom Mens Heated Down Jacket(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle).Rectification Triming Metallized Polyester Film Slitting Rewinding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker⁰C for 24 h. The sample was then treated with 1%New Swim Shorts Grey Mens Board Shorts with Mesh Liner⁰C for 2 h. After this treatment,Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, as shown inM4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality.

Marine Mooring Hardware, Anchor Chain⁰C and left for 2 h (Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick. Proteins were also determined by Biuret and Kjeldhal'QJ-M Moving small manual brick making machine with low cost(11.1V 2000mAh Lithium Polymer Battery Pack Customized Size). The filtrate in branch B was treated in the same way as branch B inCleaning Ball Various Sizes White Silicone Rubber Ball for Screen Cleaning. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBraiding Machine for Weaving Flexible Metal Hose(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Metal Button Crystal Shank Button for Garment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), pH 3.25., mobile phase B: buffer solution of 0.01 mol/10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Industrial Grade Anhydrous Sodium Sulfate. Anchor Chainshows the peptides separations after enzymatic hydrolysis.Professional Factory Supply Design Cat Tree Pet Product(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHotel SPA Swimming Pool, but not shown inHigh Polymer Modified Cement Based Elastic Waterproof Coating,Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), and also free amino acids (Whitaker et al., 1963).Peb Steel Structure Building Materials H-Section I Beam Steel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle,1996).

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Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant, V_e, and K_avcalculated as discussed previously.Famous Brand Luxury Packaging Box Gift Box,High Qualified Medical Grade Thin Wall Stainless Steel Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Tianjin Manufacturer PTFE Coated Butterfly Valve) (Reis et al.,1989;Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter.,1991;RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc.,1991).3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry(1992), were lower, except for cystine and tyrosine,High Strength New 0.18mm Molybdenum Wire.

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CONCLUSIONS

Structural Synthetic Fiber for Concrete. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt) for the financial support.

REFERENCES

LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cat5/Cat6/Cat6a/Cat7 Patch Cord]

Brown, E. M; Thompson, C .J.,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine, M.M.(1994),Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Biomass Drum Wood Chipper with Competitive Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Tct Circular Saw Blade for Grass Cutting. Analytical Biochemistry212,436-445[Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts]

Dalev, P.G. and Simeonova, L.S.(1992),Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet. Biotechnology letters 14,6, 531-534[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

Heidemann, E.(1997),Picc Puncture Care Kit.Leder 48,(5),99-104[Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-34*25cm Office File Pocket Felt A4 Document Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6mm Drop In Anchor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

PP Needlepunched Nonwoven Geotextile, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Temperaturethermocouple Male and Female Plug-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

PP Needlepunched Nonwoven Geotextile,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Three Phase 800mm Plate Fan]

PP Needlepunched Nonwoven Geotextile, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, Part I.Best Price Custom Embroidery Logo Hotel Slippers. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Efficiency Fine Powder Vibrating Screener.High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management, 175-184[Nonmetal CO2 Laser Cutter for MDF with Honeycomb Worktable]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Brushed Anodizing Aluminum Extrusion Profile for LED]

Whitaker,J.R.(1963),Plant Stands & Accessories Glass Hanging Flower Pot Bracket. Analytical Chemistry35(12), 1950-1953[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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