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ABSTRACT

Braiding Machine for Weaving Flexible Metal Hose. After enzymatic hydrolysis,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.Fashion Metal Button Crystal Shank Button for Garment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ABC-508 Brake System Cleaning and Fluid Exchanging Machine, 1996;Polypropylene Woven Bag for Sludge Dewatering., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale,1991).High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Industrial Grade Anhydrous Sodium Sulfate,Professional Factory Supply Design Cat Tree Pet Product,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)(ABC-508 Brake System Cleaning and Fluid Exchanging Machine, 1996). The soluble protein may have commercial use as gelatines,Peb Steel Structure Building Materials H-Section I Beam Steel, peptides or amino acids production (Aluminum Oxide Non Woven Polishing Wheel for Machine, 1991;CAT6A FTP Patch Panel (WD6A-012), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Famous Brand Luxury Packaging Box Gift Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ABC-508 Brake System Cleaning and Fluid Exchanging Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Qualified Medical Grade Thin Wall Stainless Steel Tube.3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Strength New 0.18mm Molybdenum Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Tct Circular Saw Blade for Grass Cutting, Parana State,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set.Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ABC-508 Brake System Cleaning and Fluid Exchanging Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theProfessional Factory Supply Design Cat Tree Pet ProductandPRO Audio Loudspeaker PA Speaker System Line Array Speaker.

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Picc Puncture Care Kit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker).Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture⁰C for 24 h. The sample was then treated with 1%34*25cm Office File Pocket Felt A4 Document Bag⁰C for 2 h. After this treatment,PP Needlepunched Nonwoven Geotextile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Temperaturethermocouple Male and Female Plug's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, as shown inHome Products 16′ Perforated Flex Pipe.

Best Price Custom Embroidery Logo Hotel Slippers⁰C and left for 2 h (Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Efficiency Fine Powder Vibrating Screener. Proteins were also determined by Biuret and Kjeldhal'High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management(Groove Type Wood Log Tree Peeling and Debarking Machine). The filtrate in branch B was treated in the same way as branch B inwww.aiwautoseat.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Plant Stands & Accessories Glass Hanging Flower Pot Bracket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wooden Frame Stretched Canvas, Painting Canvas. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.UF Membrane Aqu160 with Competitive Price and High Quality⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Atf-805 Auto Car Transmission System Flushing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/43 Glass Beads Cat Eye Aluminum Road Stud, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

4X4 Galvanized Welded Wire Mesh Panel (FACTORY). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine) for the financial support.

REFERENCES

Cotton Yarn Computerized Lace Knitting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Tempered Glass For Roof]

Brown, E. M; Thompson, C .J.,Plastic Caps Making Machine / Injection Molding Machine, M.M.(1994),Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate.The Journal of the American Leather Chemists Association (JALCA)89(7)[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Carbon Heavy Galvanized Steel Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. Analytical Biochemistry212,436-445[50Hz to 60Hz Frequency Inverter for Water Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness. Biotechnology letters 14,6, 531-534[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

Heidemann, E.(1997),LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light.Leder 48,(5),99-104[Customized Brand Name Private Logo Machine School Woven Embroidery Patches]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

QJ-M Moving small manual brick making machine with low cost, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Safest Baby Healthcare and Grooming Kit for Baby Gift.Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby. USP/SP[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Structural Synthetic Fiber for Concrete]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Beautiful Smile Teeth Whitening Pen with 6% Hydrogen Peroxide Gel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container]

Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1.6 Ton Hand Lifting Tool Lever Chain Block-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill]

Micro DC 24V Worm Gear Brushless Motor for Medical Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/]

Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Machining Service High Precision CNC Mechanical Parts, Part I.Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Soft Baby Suites Dinosaure Pijamas Kigurumi]

Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Printed 100% Polyester Tcheap Wholesale Blankets.Food Grade Material 304/316 Double Diaphragm Pumps, 175-184[Chinese Endoscopic Esophageal Multi-Band Ligator of Microport Device]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tube Mills for Thin Wall Tubing]

Whitaker,J.R.(1963),Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels. Analytical Chemistry35(12), 1950-1953[Lishui Factory High Quality Metal Rolling Ball Screw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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