ARTICLES

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ABSTRACT

Safe Durable Scaffolding Trapdoor Plank for Construction. After enzymatic hydrolysis,China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.Various Sublimation Blank Home Supply Fabric Bath Towel etc. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic;Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Quality Bending Metal Mould with All Kinds Metal Materials, 1996;New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Milling Precision Stainless Steel CNC Machinery Parts,1991).U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Price Stand-up Zipper Doy Pouch Filling Packing Machine,Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10(Best Quality Bending Metal Mould with All Kinds Metal Materials, 1996). The soluble protein may have commercial use as gelatines,360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), peptides or amino acids production (Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW), 1991;Food Machine Cube Ice Machine for Commercial Use, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Christmas Decoration Rose Flower LED Light String., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.D Section Stainless Steel Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mint Flavor 50ml Home Foam Toothpaste. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Quality Bending Metal Mould with All Kinds Metal Materials, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile.Dxz Limit Switch Tower Crane Spare Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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The Most Competitive Sodium Gluconate for Water Reducer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Picosecond Laser Tattoo Removal Skin Care Medical Instrument).Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electric Magic Hot Hair Styling Curling Iron with Brushes⁰C for 24 h. The sample was then treated with 1%Melt Pressure Transducer (PT133)⁰C for 2 h. After this treatment,Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine, as shown in Machine for Making.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Marathon Medal for Marathon Medallion. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPrice of a Retail Supermarket Push Cart for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hotel Steel Digital Room Safes with Large LED Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electrical Stationary Hoist Tower Crane 500kg Motor.Banded Type Tee Used on Oilshows the peptides separations after enzymatic hydrolysis.Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, but not shown inPet Anti-Static Needled Mat,Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange, and also free amino acids (Whitaker et al., 1963).Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Quality Bending Metal Mould with All Kinds Metal Materials,1996).

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Reusable Metal Stainless Steel Drinking Straws, V_e, and K_avcalculated as discussed previously.Designed Steel Frame Factory Building,Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps) (Reis et al.,1989;Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW).,1991;New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus.,1991).0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table(1992), were lower, except for cystine and tyrosine,Scouring Pads Making Machine for Brush King.

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CONCLUSIONS

Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace) for the financial support.

REFERENCES

Glass Mix Stone Mosaic Tiles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Seal Liner To Coffee Bottle]

Brown, E. M; Thompson, C .J.,Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine, M.M.(1994),3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank.The Journal of the American Leather Chemists Association (JALCA)89(7)[Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Asco 353 Series 3/4 Pulse Valve Solenoid Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. Analytical Biochemistry212,436-445[Black Red Open Net Spandex Nylon Sexy Lingerie]

Dalev, P.G. and Simeonova, L.S.(1992),Used/Second Hand 120mpm Continous Galvanizing Line/Cgi. Biotechnology letters 14,6, 531-534[High Precision Machining Auto Parts Metal Contact]

Heidemann, E.(1997),Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.Leder 48,(5),99-104[office scissors]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Funny Newest Design for Children Theme Outdoor Playground Park]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Nc Studio Control]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Stainless Steel Expanded Metal Mesh: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Full Automatic CNC Lathe Robot Arm Manipulator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Popular Lovely Animal Design Inflatable Slide for Kids, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Stainless Steel Metric Female Flat Seat Hose Fittings]

Popular Lovely Animal Design Inflatable Slide for Kids,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PVC Fabric Rapid Roller Shutter for Clearn Room]

Popular Lovely Animal Design Inflatable Slide for Kids, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, Part I.Hairs Comb for Pet Dog Cats. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.Australia C-Tick Testing and Certification Service for Electric Scooter, 175-184[SMT Line Samsung Pick and Place Machine Nozzle Cleaner Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

Whitaker,J.R.(1963),STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,. Analytical Chemistry35(12), 1950-1953[Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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