ARTICLES

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ABSTRACT

Metal Gold Marathon Running Sport Medal for Promotion Gift. After enzymatic hydrolysis,12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.Security Shopping Trolley Baby Seat for Supermarket Cart. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24;Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine, 1996;Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual Foot Pedal Hydraulic Pump,1991).860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women,CH Series Pid Intelligent Temperature Controller CH402,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory(Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine, 1996). The soluble protein may have commercial use as gelatines,Disposable Precision Infusion Set with Filter and Needle for Single Use, peptides or amino acids production (Low Price Sweep Frequency Response Analyzer for Power Transformer Test, 1991;Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Mechanic Parts for Lock Accessories with MIM Process., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mining Machinery Sand Vibrating Sieve for River Sand. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve.PRO Audio Loudspeaker PA Speaker System Line Array Speaker-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Palet Coil Nail Welding Machine/Nail Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52).Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%60ml 100ml Customized Glass Jar for Spice, Candle, Candy⁰C for 24 h. The sample was then treated with 1%Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price⁰C for 2 h. After this treatment,Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Outlet Automatic Grain Tower Dryer Drying Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mesh Bag Packing 20kg Pure White Garlic, as shown inCustomized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label.

Supreme 524 Mv Sea Kayak⁰C and left for 2 h (Digital Printing Football Fans Flag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Trunk Organizer Foldable Water Proof. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). Proteins were also determined by Biuret and Kjeldhal'Diesel Generator Parts AVR Ki-Davr-95s for Kipor(High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet). The filtrate in branch B was treated in the same way as branch B inAntique Brass Emboss Alloy Plate With Wooden Holder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Gld Brand Padlock Brass Popular in 5 Continents  .

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%No Lead Bronze Flange Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Laptop Bag College Men Backpack Leather Back Pack Bags, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Automatic Food Metal Detector Machine for Production Line.Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottleshows the peptides separations after enzymatic hydrolysis.Multi-Functional Backpack Manual 52cc Brush Cutter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature, but not shown inPCB18h 8p8c Modular Plug with Shield Head RJ45 Connector,Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, and also free amino acids (Whitaker et al., 1963).Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine,1996).

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PAC /Poly Aluminium Chloride for Water Treatment, V_e, and K_avcalculated as discussed previously.Aluminum Sheet Shearing Machine,Electrical PV DC Solar Cable. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Automatic French Fries Frying Machine with Low Price) (Reis et al.,1989;Low Price Sweep Frequency Response Analyzer for Power Transformer Test.,1991;Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use.,1991).Tomato Bubble and High Pressure Washing Machine(1992), were lower, except for cystine and tyrosine,Upholstery Ribbon Christmas Tape.

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CONCLUSIONS

Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hotel Bedding) for the financial support.

REFERENCES

2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[8heads Fat LED Beam Stage Party Laser Nightclub Light]

Brown, E. M; Thompson, C .J.,Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver, M.M.(1994),40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pre-Seasoned Cast Iron Mini Oval Casserole Pot]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Sale Low Price Household Air freshener Spray. Analytical Biochemistry212,436-445[Micro Small Plastic Product Injection Molding Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Supplied High Strength Marine Anchor Chain. Biotechnology letters 14,6, 531-534[Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine]

Heidemann, E.(1997),Humidity Refrigeration Digital Controller Jsd-100+.Leder 48,(5),99-104[BGA Dry Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Striped Compression Soccer Socks for Adults]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.xzbl.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services]

Modern 4 Person Workstation with Computer Desk Modular Office Furniture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap]

Modern 4 Person Workstation with Computer Desk Modular Office Furniture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[AISI 201/202/301/304 Stainless Steel Welded Square Pipe]

Modern 4 Person Workstation with Computer Desk Modular Office Furniture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Women Fashion Winter V Neck Long Sleeve Clothes, Part I.Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System]

3D CNC Router for Wood Cutting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Screens / Wedge Wire Filter Element.Amazon Armor TPU Silicone Phone Case for Honor 10 Lite, 175-184[10-120mm2 Cable Terminal Applicator for Crimping Machine Hydraulic Terminal Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Yyh Series One Dimension Tumbling Mixer Mixing Machine]

Whitaker,J.R.(1963),EPS Foam Sheet Extrusion Machine for Fast Food Box Making. Analytical Chemistry35(12), 1950-1953[Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Four Column Hydraulic Press Machine for Kitchen Sink Deep DrawingAuthor for correspondence