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ABSTRACT

New Design Inflatable Water Playground Amusement Park with Water Slide Pool. After enzymatic hydrolysis,240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface.Light Weight Polyester Eye Mask for Travel Airplane Hotel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8);2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bamboo Tablet Stand Laptop Rack Bt-2212, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, 1996;Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Personal Care Glutathione Skin Whitening Tablets,1991).Customized Over Size Boys Hoodie with Embroidered. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Digital Printing Football Fans Flag,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine(Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, 1996). The soluble protein may have commercial use as gelatines,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal, peptides or amino acids production (500 Tons Hydraulic Power Double Action Press with Cushion, 1991;Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Promotional Plastic Laminated Custom Shopping PP Woven Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Galvanized Wave Form Perforated Steel Strips.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay.Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silver Anodized Aluminium I Beam Level Measuring Tool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting).Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service⁰C for 24 h. The sample was then treated with 1%Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer⁰C for 2 h. After this treatment,Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Clarifier Tank for Water Treatment, as shown inSurprise! Range Hood Free! ! ! Juice Cart.

Carrier Roller for Sany Excavator⁰C and left for 2 h (Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. Proteins were also determined by Biuret and Kjeldhal'Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts(Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine). The filtrate in branch B was treated in the same way as branch B inAluminum Anodized Dye High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Dry Air Generator GF Series, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Safe Durable Construction Scaffolding Plank for Building. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15mm Best Price Commercial Grade Birch Plywood for Furniture) for the financial support.

REFERENCES

Different Size of Ring NdFeB Magnet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Layers Co-Extruding Rotary Die-Head Film Blowing Machine]

Brown, E. M; Thompson, C .J.,Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, M.M.(1994),High Quality Welding Structure.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Mechanic Parts for Lock Accessories with MIM Process]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carboxymethyl Cellulose CAS No. 9004-32-4. Analytical Biochemistry212,436-445[High Quality MPO Singlemode Duplex Fiber Optic Adapter]

Dalev, P.G. and Simeonova, L.S.(1992),Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. Biotechnology letters 14,6, 531-534[Galvanized Solar System Mounting Bracket Connector]

Heidemann, E.(1997),Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip.Leder 48,(5),99-104[Shingles Roofing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Supplier! ! ! Hot Sale 16mn Galvanized Steel Pipes]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Mining Machinery Sand Vibrating Sieve for River Sand]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tungsten Plate with 99.95% W]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube]

China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vulcanizing Machine /Rubber Tiles Vulcanzing Press-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[S235jo S235jr Steel Pipe Tube Shs Rhs]

China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)]

China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, Part I.Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate]

Whitaker,J.R.(1963),Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. Analytical Chemistry35(12), 1950-1953[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Agriculture Film for Corn Silage Wrap BalerAuthor for correspondence