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ABSTRACT

High Speed Roll Plastic T-Shirt Bag Making Machine. After enzymatic hydrolysis,Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine.Wholesale Black Galvanized Head Stainless Steel Screw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine;45*60cm Anti-Fatigue ESD Rubber Floor Mat; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vinyl Glove in Work & Labor Glove, 1996;Low Energy Needed Compact Flour Mill Plant for Milling Wheat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Nylon Weatherproof PA6 Strain Relief Cable Gland,1991).Steel Roller Embossed Paper 2019 New Design. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil,Factory Price DIN5685 Galvanized Marine Anchor Link Chain,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)(Vinyl Glove in Work & Labor Glove, 1996). The soluble protein may have commercial use as gelatines,6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, peptides or amino acids production (High Level Application Waterproof Plastic Box, 1991;Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Water Slide for Indoor Play Structure.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vinyl Glove in Work & Labor Glove, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1220*2440mm Concrete Formwork black film faced plywood.Washami Teeth Care for Whitening Cool Mint Toothpaste-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball, Parana State,Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine.Yyh Series One Dimension Tumbling Mixer Mixing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Vinyl Glove in Work & Labor Glove, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Eye Steam MaskandCarrier Roller for Sany Excavator.

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ODU 14 Pin Industrial Circular Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Oxide Non Woven Polishing Wheel for Machine).Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Classical New Coming Plastic Female Sunglasses⁰C for 24 h. The sample was then treated with 1%OEM Double Sided Rigid PCB Circuit Board Manufacturer⁰C for 2 h. After this treatment,Robot of World Robotics Competition. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Rope/Cable/Wire Braiding Machine with Factory Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum PCB Circuit Board SMD PCBA LED PCB, as shown inConcrete Floor Grinding Disc Machine for Four Segments.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%White Ceramic Shot Glass for Sublimation Printing - 1.5oz. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPromotional Gifts Custom Printed Silicone Wristband/Bracelet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Farrowing Crate Pig Floor Cast Iron Slat Floor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns.Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panelshows the peptides separations after enzymatic hydrolysis.55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W, but not shown inABC-508 Brake System Cleaning and Fluid Exchanging Machine,Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security, and also free amino acids (Whitaker et al., 1963).Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vinyl Glove in Work & Labor Glove,1996).

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Slip Resistant Corrugated Protection Entrance Rubber Floor MatandCAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronateshow the gel chromatographic separation of soluble protein with Sephadex G-Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block.High Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card, and another peak at 45 mL not shown inFactory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor, for peptides lower than 3000 Da.T90 High Power Latching Relay Mini Power Relay(Condell et al.,1993) .

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CONCLUSIONS

Aluminum Mould Shoe Mould Making CNC Milling Router Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag) for the financial support.

REFERENCES

High Pressure Fitting Quick Coupling Hydraulic Plug, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[etonsmtauto.com]

Brown, E. M; Thompson, C .J.,High Quality Products Insulin Syringe/ Insulin Pump with Medical Head Cover, M.M.(1994),Refrigeration Digital Temperature Controller Stc-100A.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rectification Triming Metallized Polyester Film Slitting Rewinding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Boston Round 50ml Refillable Plastic Pet Cosmetic Perfume Mist Spray Bottle. Analytical Biochemistry212,436-445[New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints]

Dalev, P.G. and Simeonova, L.S.(1992),Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies. Biotechnology letters 14,6, 531-534[Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover]

Heidemann, E.(1997),Black Color Hexagon Bolt with High Quality.Leder 48,(5),99-104[Flange Mating Sleeve No Flange Sc Green Color Simplex Single Mode/Multimode Fiber Optic Adapter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flat Pack Temperary Container House 20FT 40FT]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Engieered Veneer Faced Commericial Plywood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baochuan Welded Wire Mesh Fence]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting]

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery, Part I.Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Positive Drive Turn Belt for Spiral Freezing Equipment]

Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Single Stage High Speed Fruit and Vegetable Pulping Machine.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, 175-184[China Aluminium Bathroom Doors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Yitai Die Making 13mm Beech Rotary Plywood]

Whitaker,J.R.(1963),Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services. Analytical Chemistry35(12), 1950-1953[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass BottleAuthor for correspondence