ARTICLES

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ABSTRACT

240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface. After enzymatic hydrolysis,Light Weight Polyester Eye Mask for Travel Airplane Hotel.6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable;Bamboo Tablet Stand Laptop Rack Bt-2212; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, 1996;Personal Care Glutathione Skin Whitening Tablets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Over Size Boys Hoodie with Embroidered,1991).Digital Printing Football Fans Flag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors,Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal(Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, 1996). The soluble protein may have commercial use as gelatines,500 Tons Hydraulic Power Double Action Press with Cushion, peptides or amino acids production (Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, 1991;Promotional Plastic Laminated Custom Shopping PP Woven Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Galvanized Wave Form Perforated Steel Strips., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale.Silver Anodized Aluminium I Beam Level Measuring Tool-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank).Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer⁰C for 24 h. The sample was then treated with 1%Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail⁰C for 2 h. After this treatment,High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Clarifier Tank for Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Carrier Roller for Sany Excavator, as shown inDouble Roller Rotary Wood Peeling Machine with CE for Sale.

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes⁰C and left for 2 h (Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts. Proteins were also determined by Biuret and Kjeldhal'Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets(Factory Price High Carbon Isostatic Graphite Electrodes). The filtrate in branch B was treated in the same way as branch B inSimple Decorative Omani Design Figure Fabric Shade Standing Floor Lamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dry Air Generator GF Series⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Advanced Unlocking Tool for Locksmith, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Different Size of Ring NdFeB Magnet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top Great Wallet Genuine Leather Purse with Coin Pocket) for the financial support.

REFERENCES

Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cable Lug Sc]

Brown, E. M; Thompson, C .J.,High Quality Welding Structure, M.M.(1994),Carboxymethyl Cellulose CAS No. 9004-32-4.The Journal of the American Leather Chemists Association (JALCA)89(7)[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Eyes- Caring LED Night Light for Nursing Reading with Soft Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. Analytical Biochemistry212,436-445[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Dalev, P.G. and Simeonova, L.S.(1992),Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip. Biotechnology letters 14,6, 531-534[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

Heidemann, E.(1997),150kg Per Hour Frozen French Fries Production Line.Leder 48,(5),99-104[Cheap Rubber Flooring]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

Chemicals Flocculant Cationic Polymer Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Professional LED Display Ceramic Hair Curling Iron.CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. USP/SP[Corrugated Board Production Line]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[T90 High Power Latching Relay Mini Power Relay]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.clin-ele.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Safe Durable Construction Scaffolding Plank for Building]

Vulcanizing Machine /Rubber Tiles Vulcanzing Press, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

Vulcanizing Machine /Rubber Tiles Vulcanzing Press,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

Vulcanizing Machine /Rubber Tiles Vulcanzing Press, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, Part I.Omni Newest Manager Desk Office Smart Office Table Office Staff Table. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps.Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage, 175-184[Cheap Interlocking Foam Mats]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Whitaker,J.R.(1963),Full-Auto CNC Cutting Machine for Architecture Glass Sheet. Analytical Chemistry35(12), 1950-1953[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper PullAuthor for correspondence