ARTICLES

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ABSTRACT

Maxdrill 64mm T38 Thread Button Drill Bit Top Hammer Drill Rock Mining Bits. After enzymatic hydrolysis,3HP Industrial Cooling System Water Cooled Chiller.OEM/ODM Custom Made Muslim Bridal Wedding Gown Dress. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale High Quality Decoration Oil Painting, Home Decoration Painting, Art Painting (abstract sailing oil painting), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, 1996;32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Kara Two-Way Dual 8′′ Line Array PRO Audio System Powerful PA Speaker,1991).Corrugated Board Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Magnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive,Car Air Conditional Compressor Magetic Clutch 5pk,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Engieered Veneer Faced Commericial Plywood(Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, 1996). The soluble protein may have commercial use as gelatines,Hex Thin Head Nut, peptides or amino acids production (Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2, 1991;Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Spare Parts for Caterpillar Construction Machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50 Grams Ozone Producing Device for Makeup Toner Producing Plant. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flat Pack Temperary Container House 20FT 40FT.Sweat Suit for Women Three Piece Tracksuit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cold Rolled Stainless Steel Strip /Coils 410/430/409(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System, Parana State,Folding Round Picnic Caravan Table RV Picnic Table Camping Table.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theManufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of BackingandFast Supplier Juice Sealing Film Wrapping Packing Machine Production Line.

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PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Classical New Coming Plastic Female Sunglasses).Blue Steel Packing Strip(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Powder and Wax Brush with Wooden Handle⁰C for 24 h. The sample was then treated with 1%Cheap Price Paper Straws Individually Wrapped Drinking Straw⁰C for 2 h. After this treatment,ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose, as shown inSx Sm Fiber Optic MPO to MPO Adapter with Flange Type.

Smelting and Heating Induction Machine (GW-HY187)⁰C and left for 2 h (Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Home Products 16′ Perforated Flex Pipe. Proteins were also determined by Biuret and Kjeldhal'1220 Fork Length 3-Wheel Electric Forklift Truck(Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse). The filtrate in branch B was treated in the same way as branch B inBuy Lidocaine High Quality 99% Chemical Pain Relief Powder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%FM USB Radio Audio Power Amplifier for Home Bar Club. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFunctional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glow Powder for Luminous Coating⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/700 mm RP HP UHP Graphite Electrode Sold at Factory Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Gsx750f Gsf1200 Bandit 01-06 Carb Repair KitandUnion Metal Steel Building and Construction Material. Enzymatic (Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller)450ml Sublimation Stainless Steel Milk Bottle (Colorful)(sulfuric acid)Lower Noise Colled Rolled Ball Screw(Si3n4 Full Ceramic  Bearing  608 Size 8X22X7, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Fused Quartz Powder 325mesh 500mesh 1250mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig) for the financial support.

REFERENCES

Various Types of Plastic Hooks Mould From Manufacturers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium Die Casting Machine Price]

Brown, E. M; Thompson, C .J.,New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle, M.M.(1994),10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mint Flavor 50ml Home Foam Toothpaste]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift. Analytical Biochemistry212,436-445[Fly]

Dalev, P.G. and Simeonova, L.S.(1992),Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India. Biotechnology letters 14,6, 531-534[Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine]

Heidemann, E.(1997),Soft Baby Suites Dinosaure Pijamas Kigurumi.Leder 48,(5),99-104[Bluetooth Light Bulb]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cable Tray with ′t′ Slots]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blood Pressure Monitor Cuff]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Prize Solar PV Mounting Panel System for Ground]

E171 Standrad Titanium Dioxide Food Grade A200, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Elbow Forming Machine Gorelocker HVAC Duct Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

E171 Standrad Titanium Dioxide Food Grade A200,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r]

E171 Standrad Titanium Dioxide Food Grade A200, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh, Part I.Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sweat Suit for Women Three Piece Tracksuit]

L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric.304 Stainless Steel Anti-Corrosion Welded Wire Mesh, 175-184[Zirconia Sintering Furnace]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Over Size Boys Hoodie with Embroidered]

Whitaker,J.R.(1963),Custom Made Colorful Sublimation Womens Yoga Pants Wholesale. Analytical Chemistry35(12), 1950-1953[Diaphragm Valve for Bag Filter Since 1992]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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