ARTICLES

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^IICNPq/RHAE/biotechnology-Supreme 524 Mv Sea Kayak. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Trunk Organizer Foldable Water Proof

ABSTRACT

100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). After enzymatic hydrolysis,Diesel Generator Parts AVR Ki-Davr-95s for Kipor.Gld Brand Padlock Brass Popular in 5 Continents  . Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;No Lead Bronze Flange Manufacturer;Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Snsc Fd30 Side Shift New Diesel Forklift 3ton, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), 1996;Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laptop Bag College Men Backpack Leather Back Pack Bags,1991).Pet Dog Grooming Rake Dematting Tool Shedding Comb. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Apparel Applique Round Patch School Club Embroidery Collar Patch,Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058(Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), 1996). The soluble protein may have commercial use as gelatines,Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror, peptides or amino acids production (Automatic Food Metal Detector Machine for Production Line, 1991;Multi-Functional Backpack Manual 52cc Brush Cutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White Cotton Satin Hotel Duvet Cover (DPFB8055).Small Cleaning Brush ESD Anti Static Brush ESD Brush Black-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PAC /Poly Aluminium Chloride for Water Treatment(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Menthol E Liquid E Juice for EGO E Cigarettes).40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Sale Low Price Household Air freshener Spray⁰C for 24 h. The sample was then treated with 1%Factory Supplied High Strength Marine Anchor Chain⁰C for 2 h. After this treatment,Humidity Refrigeration Digital Controller Jsd-100+. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Elegant Europe Dessign Pillow Top Mattress's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)8 Gang Waterproof LED Rocker Switch Panel for Marine Boat Caravan (AP8), as shown inFactory OEM CNC Machined Gear Motor Shaft.

Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts⁰C and left for 2 h (High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modern 4 Person Workstation with Computer Desk Modular Office Furniture. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill. Proteins were also determined by Biuret and Kjeldhal'Women Fashion Winter V Neck Long Sleeve Clothes( COB Stn LCD Display with 8080 Interface). The filtrate in branch B was treated in the same way as branch B inHigh Quality 3 Ply Surgical Disposable Nonwoven Face Mask Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full-Automatic Production Line for Continuous Draw-out Wet Wipe Wet Wipes Machine Baby Wipes Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3D CNC Router for Wood Cutting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Screens / Wedge Wire Filter Element(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.EPS Foam Sheet Extrusion Machine for Fast Food Box Making⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Easy Operational Cup Cake Filling Machine.Carboxy Terminal Collagen Crosslinks Elisa Kitsshows the peptides separations after enzymatic hydrolysis.Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPopular Custom School Uniform Woven Badge Patch with Merrow Border, but not shown inPump Flower Sprinkler with Automatic Watering System,Folding Play Mat Crawl Play Mat, and also free amino acids (Whitaker et al., 1963).Wooden Grain Perforated Panel for Metal Mesh Curtain Wall. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117),1996).

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Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant, V_e, and K_avcalculated as discussed previously.Security RFID Protection E-Shield Card RFID Blocking Card for Proximity Identification Field,Low Carbon Heavy Galvanized Steel Wire. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup) (Reis et al.,1989;Automatic Food Metal Detector Machine for Production Line.,1991;Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type.,1991).Long Distance Measurement Power Laser Digital Meter(1992), were lower, except for cystine and tyrosine,Wood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturer.

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CONCLUSIONS

Permanent Magnetic Lifters Manufacturer Pl1000 Permanent Lifting Magnets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tomato Bubble and High Pressure Washing Machine) for the financial support.

REFERENCES

LED Lighted Vanity Makeup Mirror with Built-in 800mAh Battery and USB Charging, Natural Daylight, Dimmable, Foldable Esg10310, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automobile Usage Precise Tube Mill Line]

Brown, E. M; Thompson, C .J.,Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key, M.M.(1994),Eko Custom Color Marked Self-Adhesive Hook and Loop Cable Tie for Wire Management.The Journal of the American Leather Chemists Association (JALCA)89(7)[Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Surprise! Range Hood Free! ! ! Juice Cart]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality CO2 Laser Marking Machine with Ce. Analytical Biochemistry212,436-445[Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop]

Dalev, P.G. and Simeonova, L.S.(1992),New Design Inclined Plane 18W LED Wall Washer Lighting. Biotechnology letters 14,6, 531-534[Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine]

Heidemann, E.(1997),2ton R Type Electric Wire Rope Hoist with Single Speed.Leder 48,(5),99-104[12v bürstenloser DC-Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Foldable Esg10310]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Adhesive CAS 9003-35-4 Phenolic Resin: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2+1 Flavors Soft Ice Cream Machine with Pre-Cooling]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dish Drying Rack and Drainboard Set]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Filter Strainer]

Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacture Best Choice Liquid Flocculant for Ink Removal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes]

Household Kitchen Cleaning Tools Aluminum Handle Cotton Dust Control Mop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Dipped Galvanized Oval Wire Brazil, Part I.Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh]

Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit.Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1), 175-184[m.wanlmedical.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System]

Whitaker,J.R.(1963),Aluminum Foil PE Bottle Cap Induction Seal Liner. Analytical Chemistry35(12), 1950-1953[XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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