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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Decorative Aluminum Solid Panel for Commercial Building. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing

ABSTRACT

Di DIN3352 Double Orifice Automatic Air Release Valve Dn65. After enzymatic hydrolysis,Magic Hair Twist   Curl Sponge Brush.Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bimetallic Thermometer Industrial Temperature Gauge, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (19 Network Cabinet with Lockable Rear Door, 1996;Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (175mm Diamond Cutting Blade for Granite&Tiles,1991).Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PU-Flex Duct Hose for Transporting Dust, Granules and Shavings,Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Uprigth Touch-Screen Red Wine Chiller(19 Network Cabinet with Lockable Rear Door, 1996). The soluble protein may have commercial use as gelatines,Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, peptides or amino acids production (Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, 1991;Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Groove Type Wood Log Tree Peeling and Debarking Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality CE Digital Display Adjustable Power Supply. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (19 Network Cabinet with Lockable Rear Door, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires.Parcel Tape Adhesive OPP Packing Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Efficiency Non-Expensive Cracking Agent(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, Parana State,Cummins ISBE truck diesel engine motor parts 3940059 camshaft bushing.High Tensile Strength Galvanized Steel Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (19 Network Cabinet with Lockable Rear Door, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIntelligent Regulating Sanitary Diaphram Valves with Flow Control Head for BeverageandGood Quality Pillow for Pregnant Wome Body Pregnant Pillow Case.

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24 Volt Fire Alarm Strobe Light Horn Strobe Sounder(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing).from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price PVC Coated Fiberglass Mesh Insect Window Screen⁰C for 24 h. The sample was then treated with 1%Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application⁰C for 2 h. After this treatment,Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory OEM CNC Machined Gear Motor Shaft's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage, as shown inOEM 15PCS Electric Face Makeup Brush with Ce Certificate.

Pink French Loop Terry Knit Fabric for Jacket⁰C and left for 2 h (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. Proteins were also determined by Biuret and Kjeldhal'500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel(Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe). The filtrate in branch B was treated in the same way as branch B inHigh Quality 19′′ Standard Installation Wall Mount DDF Network Cabinet. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBuiltin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cute Pink Cashmere Soft Plush Lining Warm Bootie, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3kw 24V 310mm Vertical Agv Drive Wheel Assembly, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Genuine Leather Credit Card Mens Wallet.Washami Teeth Care for Whitening Cool Mint Toothpasteshows the peptides separations after enzymatic hydrolysis.Yitai Die Making 13mm Beech Rotary Plywood(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb, but not shown inPrice of Automatic Sludge Dewatering Machine,Gear Rolling Machine Zh28/25 Spline Hobbing Machine, and also free amino acids (Whitaker et al., 1963).OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (19 Network Cabinet with Lockable Rear Door,1996).

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CONCLUSIONS

OEM 15PCS Electric Face Makeup Brush with Ce Certificate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Striped Compression Soccer Socks for Adults) for the financial support.

REFERENCES

New Styles Fashion Erotic Women Underwear Sexy Lingerie, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Finger Stripes]

Brown, E. M; Thompson, C .J.,on Site Use Psa Oxygen Plant Oxygen Cylinder Filling Machine Hot Sale, M.M.(1994),Stand Hot and Cold Normal Water Dispenser with Refrigerator.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wooden Grain Perforated Panel for Metal Mesh Curtain Wall]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Construction Welding Rods Electrode E6013 E7018 E7016. Analytical Biochemistry212,436-445[Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair]

Dalev, P.G. and Simeonova, L.S.(1992),High Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process. Biotechnology letters 14,6, 531-534[ 316L)]

Heidemann, E.(1997),Day & Night Vision 40X60 Hunting Camping Hiking HD Optical Monocular.Leder 48,(5),99-104[Credit Card RFID Card or Passport Blocking Sleeves for Personal Info Protection]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Catering Use Cafe Use Tolix Outdoor Metal Dining Chair Restaurant]

Carbon Graphite Crucible, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Lydite Fence Reel Reel with Extension Arms for Farm Fencing.1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio. USP/SP[22 High Rubber Mixing Mill]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized 3D Medal for Religious Medal Gift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[The Ductile Iron Casting Parallel Double Disc Gate Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1]

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dbry-320 Christmas Stickers Labels Printing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Temperaturethermocouple Male and Female Plug]

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle]

Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor, Part I.Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electrical PV DC Solar Cable]

14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Strong Cleaning Bow-Tie Scourer Pad Shape Abrasive Green Scouring Pad.Petrol Chain Saw Wood Cutting Machine 38cc Chainsaw CS3800, 175-184[Customized 2/4/6 Layers Flexible Circuit Board PCB Factory]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale]

Whitaker,J.R.(1963),Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope. Analytical Chemistry35(12), 1950-1953[High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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