ARTICLES

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ABSTRACT

2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt. After enzymatic hydrolysis,Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo).Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rear Wheel Exchangeable Aluminum Manual Wheelchair;90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Graphic 128X128 Dots Cog LCD, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, 1996;Tcn Sex Toy Vending Machines for Sale S800-10., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Silver 8 Inch Double Thick Round Cake Board,1991).High Precision Machining Auto Parts Metal Contact. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Paper Sticky Lint Remover Roller/Pet Hair Roller(Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, 1996). The soluble protein may have commercial use as gelatines,Safe Durable Scaffolding Trapdoor Plank for Construction, peptides or amino acids production (China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, 1991;Various Sublimation Blank Home Supply Fabric Bath Towel etc, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Quality Bending Metal Mould with All Kinds Metal Materials.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Milling Precision Stainless Steel CNC Machinery Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Food Machine Cube Ice Machine for Commercial Use(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (11kg 24L Low Pressure LPG Gas Cylinder).Christmas Decoration Rose Flower LED Light String(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%D Section Stainless Steel Tube⁰C for 24 h. The sample was then treated with 1%Mint Flavor 50ml Home Foam Toothpaste⁰C for 2 h. After this treatment,125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dxz Limit Switch Tower Crane Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, as shown inAluminum Forging Cast Metal Sand Die Casting.

High-End Business Magnet Leather Ring Binder Notebook⁰C and left for 2 h (High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. Proteins were also determined by Biuret and Kjeldhal'Eyes- Caring LED Night Light for Nursing Reading with Soft Light(Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers). The filtrate in branch B was treated in the same way as branch B inFashionable Men′s Stainless Steel Wrist Watch with Chronograph Quartz Movement (JY-ST070). The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Bearing with Housing Bearing / Pillow Block Bearing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avThe Most Competitive Sodium Gluconate for Water Reducer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Magic Hot Hair Styling Curling Iron with Brushes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Melt Pressure Transducer (PT133), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

6LTR Stainless Steel Foam Empty Fire Extinguisher CylinderandWomen Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag. Enzymatic (Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine)Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game(sulfuric acid)Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662(Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord, flow rate of 0.1 mL/min., and l = 620 nm.

Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck.Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Formingshows the peptides separations after enzymatic hydrolysis.Durable Aluminum Flag Pole with Dome Top Tangle Free Finial(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDn32 (11/4) BSPT Thread Air Actuated Piston Valves, but not shown in32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Office/Hotels/Shopping Mall,Customized Marathon Medal for Marathon Medallion, and also free amino acids (Whitaker et al., 1963).Price of a Retail Supermarket Push Cart for Sale. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen,1996).

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Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, V_e, and K_avcalculated as discussed previously.Biogas/Methane Purification System/Desulfurization/Scrubber Tower,Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Elbow Forming Machine Gorelocker HVAC Duct Making Machine) (Reis et al.,1989;China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display.,1991;Tcn Sex Toy Vending Machines for Sale S800-10.,1991).Popular Custom School Uniform Woven Badge Patch with Merrow Border(1992), were lower, except for cystine and tyrosine,1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor.

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CONCLUSIONS

Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21) for the financial support.

REFERENCES

Electrical Stationary Hoist Tower Crane 500kg Motor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medium Voltage 15kv Urd Underground Copper/Aluminum Conductor Power Cable]

Brown, E. M; Thompson, C .J.,Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, M.M.(1994),Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange.The Journal of the American Leather Chemists Association (JALCA)89(7)[Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. Analytical Biochemistry212,436-445[Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles]

Dalev, P.G. and Simeonova, L.S.(1992),Flow Wrapper/ Bakery Moon Cake Packing Machine. Biotechnology letters 14,6, 531-534[Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)]

Heidemann, E.(1997),Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine.Leder 48,(5),99-104[Shopping Mall Auto Indoor/Outdoor Moving Sidewalk Walk Walkway Ramps with High Quality]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bathroom False Plastic Board Interior Decorative 3D Wall Panels]

Tube Mills for Thin Wall Tubing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh.Reusable Metal Stainless Steel Drinking Straws. USP/SP[Steel Wire Rope Pulling Hoist or Manual Lifting Tirfor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Designed Steel Frame Factory Building: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Designed Steel Frame Factory Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tws Mini Earphone For Music]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW]

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[By6-8-200-L Automatic Doypack Premade Pouch Packing Machine]

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6 Inch Plastic Breathing Cap for The IBC Valve]

Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Scouring Pads Making Machine for Brush King, Part I.Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Carts]

Glass Mix Stone Mosaic Tiles, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine.3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, 175-184[Semi Trailer Boogie Suspension Trailer Bogie]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Customized Chilled Cast Iron Roll]

Whitaker,J.R.(1963),Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net. Analytical Chemistry35(12), 1950-1953[Water Slide for Indoor Play Structure]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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