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ABSTRACT

18V Lithium Battery Powered Cordless Drill. After enzymatic hydrolysis,High Density Rock Wool Wall Board with Thermal Insulation Performance.PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China PAC Water Flocculating Agent for Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Price High Carbon Isostatic Graphite Electrodes, 1996;Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film,1991).8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9,Powder/Spices Powder Packing Machine with Four/Three Side Seal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black Phosphated Bugle Head Csk Head Drywall Screw(Factory Price High Carbon Isostatic Graphite Electrodes, 1996). The soluble protein may have commercial use as gelatines,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine, peptides or amino acids production (China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, 1991;Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6 Inch Plastic Breathing Cap for The IBC Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Price High Carbon Isostatic Graphite Electrodes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacture Best Choice Liquid Flocculant for Ink Removal).21.5′′ Wall Mount Display Capacitive Touch Monitor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pagoda Gazebo Tent in Guangzhou for Sale⁰C for 24 h. The sample was then treated with 1%Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe⁰C for 2 h. After this treatment,Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Small Fridge for Showcase's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Microwave Motion Sensor Module Doppler Radar Detector (HW-M10), as shown in5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type.

Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile⁰C and left for 2 h (Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation. Proteins were also determined by Biuret and Kjeldhal'Spare Parts Custom Injection Plastic Moulding Manufacturer(Professional Rope/Cable/Wire Braiding Machine with Factory Price). The filtrate in branch B was treated in the same way as branch B in50Hz/60Hz Low Frequency Home Power Inverter 5000W. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKingdomway Brand Vitamin a 1000(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Speed Roll Plastic T-Shirt Bag Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Black Galvanized Head Stainless Steel Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Washami Teeth Care for Whitening Cool Mint Toothpaste. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Freshwater Pearl Butterfly Flower Bride Hairpin) for the financial support.

REFERENCES

Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Industrial Cooling Tower System]

Brown, E. M; Thompson, C .J.,Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, M.M.(1994),SHXJ1500 CNC Diamond Wire Stone Cutting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ACP Aluminium Cmposite Panel Cladding Extrusions]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hotel Bedding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag. Analytical Biochemistry212,436-445[Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. Biotechnology letters 14,6, 531-534[Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events]

Heidemann, E.(1997),Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.Leder 48,(5),99-104[m.frontop.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine]

Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Yyh Series One Dimension Tumbling Mixer Mixing Machine.ODU 14 Pin Industrial Circular Connector. USP/SP[China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Leak Proof]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lady Wrist Watch Stainless Steel Automatic Watch with Leather Strap (71174)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Classical New Coming Plastic Female Sunglasses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Double Sided Rigid PCB Circuit Board Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Classical New Coming Plastic Female Sunglasses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Classical New Coming Plastic Female Sunglasses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Robot of World Robotics Competition, Part I.Professional Rope/Cable/Wire Braiding Machine with Factory Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Aluminum PCB Circuit Board SMD PCBA LED PCB, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).Concrete Pump Sponge Cleaning Ball for Concrete Pump Truck, 175-184[Compression Knee Brace]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Whitaker,J.R.(1963),China Supplier Steel Machine Bellows Cover Telescopic Shield Cover. Analytical Chemistry35(12), 1950-1953[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical CartAuthor for correspondence