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ABSTRACT

Professional Bb Trumpet (ATR6506). After enzymatic hydrolysis,Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines.3.5ton Chinese Engine Gasoline Forklift (FG35T). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof Covering Material Interior Wall Composite Panel, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Tall/Large Cube Glass Terrarium Planter Gift Card Holder, 1996;Venlo Type Flower Multi Span Commercial Glass Greenhouses Used., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (XPS Extruded Polystyrene Foam Board,1991).Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth,Kh No Fried Automatic Potato Chips Making Machines,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Deep Hole Cylinder Boring Machine(Tall/Large Cube Glass Terrarium Planter Gift Card Holder, 1996). The soluble protein may have commercial use as gelatines,Good Prize Solar PV Mounting Panel System for Ground, peptides or amino acids production (Electric Heavy Duty Rail Guided Vehicle for Transportation, 1991;Sower Vacuum Emulsifying Machine for Cosmetic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ASTM A240 309s Stainless Steel Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Invt Bd 3/5kw Hybrid Solar Power Inverter for Solar Power System Home.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Car Mattress Back Inflatable Air Bed Car. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Tall/Large Cube Glass Terrarium Planter Gift Card Holder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum.Automatic Liquid Tomato Paste Sauce Packaging Bag Pouch Packing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Embossed Buttons Membrane Switch with FPC Circuit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chemicals Flocculant Cationic Polymer Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Metal Separating Gold Brass Zippers for Sale).Ycom USB Charger and Data Cable with Aluminum for Type C(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design Inflatable Water Playground Amusement Park with Water Slide Pool⁰C for 24 h. The sample was then treated with 1%240 * 64 Graphic LCD Screen, IC: LC7981, COB Stn LCD Display with 8080 Interface⁰C for 2 h. After this treatment,Light Weight Polyester Eye Mask for Travel Airplane Hotel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6 Seats Electric Club Car Golf Cart by Marshell (DG-C6-8)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2.5mm2 4mm² 6mm² 10mm² TUV 2pfg1169 Approved Double Insulated PV Solar Electric Power Cable, as shown inClassical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159).

Bamboo Tablet Stand Laptop Rack Bt-2212⁰C and left for 2 h (Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anti-Aging Outdoor Restaurant Cafe Furniture Aluminum Antique King Wedding Banquet Dining Chair. Proteins were also determined by Biuret and Kjeldhal'Personal Care Glutathione Skin Whitening Tablets(Dia. 5.5mm Bonding ND: YAG Laser Rod Lens). The filtrate in branch B was treated in the same way as branch B in15t Low Temperature Grain Drying Machine Paddy Dryer for Grain. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Over Size Boys Hoodie with Embroidered.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Digital Printing Football Fans Flag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Cost Safety Guarantee Storage Silo with High-Efficient Conveyors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/500 Tons Hydraulic Power Double Action Press with Cushion, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit) for the financial support.

REFERENCES

Stainless Steel Clarifier Tank for Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Brown, E. M; Thompson, C .J.,Carrier Roller for Sany Excavator, M.M.(1994),LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Yitai Die Making 13mm Beech Rotary Plywood]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine. Analytical Biochemistry212,436-445[PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle]

Dalev, P.G. and Simeonova, L.S.(1992),Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts. Biotechnology letters 14,6, 531-534[Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51]

Heidemann, E.(1997),Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets.Leder 48,(5),99-104[Furniture Fittings Plastic Shoes Rack for Shoes Cabinet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Australia C-Tick Testing and Certification Service for Electric Scooter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Potassium T-Butoxide Potassium Tert-Butanolate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

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Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, Part I.Advanced Unlocking Tool for Locksmith. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[15W Aquarium Submersible UV Lamp Durable Germicidal Lamp]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Digital Programmable Ntc Temperature Controller]

Whitaker,J.R.(1963),Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. Analytical Chemistry35(12), 1950-1953[Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody BagAuthor for correspondence