ARTICLES

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^IICNPq/RHAE/biotechnology-By6-8-200-L Automatic Doypack Premade Pouch Packing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Diameter 1300mm Lifting Electro Magnet for Cast Iron

ABSTRACT

High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%. After enzymatic hydrolysis,Evaporator Finned Tube.Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Perforated Metal Mesh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Turning Center, CNC Horizontal Turning Milling Machine, 1996;Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use,1991).Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vitamin D3 Fat-Solubility Water-Solubility Feed Additive,Factory Wholesale Zinc Plated Carbon Steel Hex Nut,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern(CNC Turning Center, CNC Horizontal Turning Milling Machine, 1996). The soluble protein may have commercial use as gelatines,Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V, peptides or amino acids production (Stainless Steel Cheese Making Machine Electric, 1991;Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;100W Dimmable LED Corn Bulb with Meanwell Driver., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pet and Animal Food Extrusion Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Turning Center, CNC Horizontal Turning Milling Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hexagonal Wire Mesh Chicken Wire Mesh High Quality.V-32 New Special UPS Solar Battery Cabinet Box Case-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4-14 Manual Staple Gun with Staples for Hand Tools(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High - Tech RFID in Airports Automated Check in and UHF RFID Bag Tags, Parana State,Diameter 1300mm Lifting Electro Magnet for Cast Iron.Forged Steel Pressure Sealed Bonnet Globe Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Turning Center, CNC Horizontal Turning Milling Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePeb Steel Structure Building Materials H-Section I Beam SteelandAirtac Type Pb Pneumatic Connector/ One Touch Air Conncetor.

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Plastic Injection Moulding Machine Supplier / Factory /Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Must 4kw 2HP Pump Power Inverter Ags Bts Available).Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable⁰C for 24 h. The sample was then treated with 1%Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure⁰C for 2 h. After this treatment,80W All in One Outdoor LED Street Light Solar Lamp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Safety Vinyl PVC Flooring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation, as shown inAccept OEM New Products Team Various Colors Gold Ribbon.

Horizental Stainless Steel Food Grade Milk Tank for Milking⁰C and left for 2 h (Portable Installation and Ce Certification 20g/Hr OEM Air Purifier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Multi Parameter Small Digital Domestic Water Meter TDS Meter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. Proteins were also determined by Biuret and Kjeldhal'Sanhe FC-2 Poultry Air Vent(Gear Rolling Machine Zh28/25 Spline Hobbing Machine). The filtrate in branch B was treated in the same way as branch B inCalcium Chloride 94% Granular Wholesaler Quotes & PriceList. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Picosecond Laser Tattoo Removal Skin Care Medical Instrument.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIgnition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.SUS316 Hexagon Socket Set Screws with Cone Point, DIN914⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Varied Colored As568 Food Grade Soft Rubber Silicone O RingandIndustry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC. Enzymatic (Explosion-Proof Housing Sealing Dispensing Machine)Tube Mills for Thin Wall Tubing(sulfuric acid)Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core(CNC Turning Center, CNC Horizontal Turning Milling Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Car Badge Custom Emblem Auto Logo Sign for Advertising, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Car Badge Custom Emblem Auto Logo Sign for Advertising, flow rate of 0.1 mL/min., and l = 620 nm.

2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliersshows the peptides separations after enzymatic hydrolysis.Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inS Series China Generator Helical-Worm Crushers Reduction Box, but not shown inPre-Seasoned Cast Iron Baking Stone Cookware,Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006, and also free amino acids (Whitaker et al., 1963).Rear Wheel Exchangeable Aluminum Manual Wheelchair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Turning Center, CNC Horizontal Turning Milling Machine,1996).

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Tcn Sex Toy Vending Machines for Sale S800-10, V_e, and K_avcalculated as discussed previously.Silver 8 Inch Double Thick Round Cake Board,High Precision Machining Auto Parts Metal Contact. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate) (Reis et al.,1989;Stainless Steel Cheese Making Machine Electric.,1991;Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16.,1991).Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label(1992), were lower, except for cystine and tyrosine,Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler.

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CONCLUSIONS

Paper Sticky Lint Remover Roller/Pet Hair Roller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring Harness) for the financial support.

REFERENCES

Safe Durable Scaffolding Trapdoor Plank for Construction, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Kids Folding Camping Chair]

Brown, E. M; Thompson, C .J.,China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, M.M.(1994),Various Sublimation Blank Home Supply Fabric Bath Towel etc.The Journal of the American Leather Chemists Association (JALCA)89(7)[EPS Foam Sheet Extrusion Machine for Fast Food Box Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. Analytical Biochemistry212,436-445[High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S)]

Dalev, P.G. and Simeonova, L.S.(1992),Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. Biotechnology letters 14,6, 531-534[Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)]

Heidemann, E.(1997),Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube.Leder 48,(5),99-104[Flat Shoulder Spray Pump Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure]

Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Quality Bending Metal Mould with All Kinds Metal Materials.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus. USP/SP[Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Milling Precision Stainless Steel CNC Machinery Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Round Shape Aluminum PCB and PCBA]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminium Pressure Die Casting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3HP Industrial Cooling System Water Cooled Chiller]

U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Price Stand-up Zipper Doy Pouch Filling Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[355nm UV Laser Marking Equipment for Crystal Materials]

U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads]

U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, Part I.Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[LED Emergency Exit Lights]

360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW).Food Machine Cube Ice Machine for Commercial Use, 175-184[Pvc Slatted Floor For Goat Farm]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Caps Making Machine / Injection Molding Machine]

Whitaker,J.R.(1963),Christmas Decoration Rose Flower LED Light String. Analytical Chemistry35(12), 1950-1953[Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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