ARTICLES

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ABSTRACT

Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer. After enzymatic hydrolysis,Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail.High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Clarifier Tank for Water Treatment;Carrier Roller for Sany Excavator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 1996;Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets,1991).Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle,High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce(Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 1996). The soluble protein may have commercial use as gelatines,Dry Air Generator GF Series, peptides or amino acids production (Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, 1991;Advanced Unlocking Tool for Locksmith, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Screen Printing Materials and Equipments Screen Printing Frames., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Supplier Hard Ware Fittings Gas Spring for Tool Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve).PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road⁰C for 24 h. The sample was then treated with 1%High Speed Copper Wire Braiding Making Machine⁰C for 2 h. After this treatment,Safe Durable Construction Scaffolding Plank for Building. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Different Size of Ring NdFeB Magnet's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine, as shown inWhite Ceramic Shot Glass for Sublimation Printing - 1.5oz.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Professional LED Display Ceramic Hair Curling Iron. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vulcanizing Machine /Rubber Tiles Vulcanzing Press⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Famous Brand Luxury Packaging Box Gift BoxandOmni Newest Manager Desk Office Smart Office Table Office Staff Table. Enzymatic (6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap)30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps(sulfuric acid)Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage(Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Full-Auto CNC Cutting Machine for Architecture Glass Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Full-Auto CNC Cutting Machine for Architecture Glass Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product.Full-Auto CNC Cutting Machine for Architecture Glass Sheetshows the peptides separations after enzymatic hydrolysis.Hot Forming Pushing Elbow Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, but not shown inHeat Pump Food Cabinet Dog Food Pet Food Dryer Machine,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, and also free amino acids (Whitaker et al., 1963).Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine,1996).

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CONCLUSIONS

Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches) for the financial support.

REFERENCES

Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Supply Football Sport Fashion Sneaker Man Soccer Shoes]

Brown, E. M; Thompson, C .J.,High Borosilicate Glass Baking Dish Set Clear, M.M.(1994),G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen.The Journal of the American Leather Chemists Association (JALCA)89(7)[Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Embossing Roller for Tissue Paper. Analytical Biochemistry212,436-445[Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. Biotechnology letters 14,6, 531-534[High Precision C45 Steel CNC Lathe Parts]

Heidemann, E.(1997),Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.Leder 48,(5),99-104[Tartan Plaid Scarf]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb]

Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Colorful  Reflective Sticker Reflective Tape.Concrete Spare Part Electrical System Central Distribution Box. USP/SP[100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Angle Bar 5052]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck]

50Hz to 60Hz Frequency Inverter for Water Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree]

50Hz to 60Hz Frequency Inverter for Water Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Steel Roller Embossed Paper 2019 New Design]

50Hz to 60Hz Frequency Inverter for Water Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), Part I.4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm]

Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Cabinet Construction Board, White 18mm PVC Foam Board.2019 New Portable Mini Dental X-ray Machine, 175-184[Standard USB Mini Fan for Power Bank and Computer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[No Lead Bronze Flange Manufacturer]

Whitaker,J.R.(1963),Ss Reduced Port 2000wog 1PC Screwed Ball Valve. Analytical Chemistry35(12), 1950-1953[1220 Fork Length 3-Wheel Electric Forklift Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Miniature Ball Bearing (616)Author for correspondence