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ABSTRACT

2 Round Sash Pulley for Chain - Brass (PVD). After enzymatic hydrolysis,Anodized Aluminum Expanded Metal Mesh Competitive Price.Hot DIP Galvanized G2150 D Ring Shackle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair;Round Shape Aluminum PCB and PCBA; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.36PCS LED Gold Matrix Stage Zoom Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, 1996;Chicken Cage Mesh Hexagonal Wire Mesh Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (110/220V Handle Mild Steel Welding Machine PCB Board 160A,1991).Swbf10b3SL-3station Multi-Station Cold Heading Machinen and Bolt Forging Former for Screw - Metal Fastener -Metal Fabrication -Nail -Rolling Forming. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Enamel Cast Iron Mini Casserole Cast Iron Cocotte,Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Energy Saving Small Air Conitioner Air Source Heat Pump Manufacturer(Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, 1996). The soluble protein may have commercial use as gelatines,Sex Toy Flirting Anal Plug Five Lines with Beads Handle Type Extra-Long Anal Plug Can Deeply Stimulate The Anus of Both Men and Women Plug, peptides or amino acids production (Automatic Cashew Processing Cashew Nuts Shelling Peeling Machine, 1991;CNC System Air Duct Line5 Make Machine for HVAC, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Titanium Dioxide Anatase for Paper Making., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.40A C Electronic Air Circuit Breaker with Dz47-4p.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pipe Thermostat with Sensor Ce Certificate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Walking Long Distance Surveying Instrument Meter Measuring Wheel.Chinese Supplier of PVC Profile-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tianjin Manufacturer PTFE Coated Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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ALS Stage 5W RGB Animation Laser Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Powder and Wax Brush with Wooden Handle).Multifunctional Solar Powered Safety Hammer LED Flashlight, Alarm Fescape Rescue LED Torch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light⁰C for 24 h. The sample was then treated with 1%Pet Anti-Static Needled Mat⁰C for 2 h. After this treatment,Dz/Bt-80h Multifunctional Full Automatic Cartoning Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Sheet Metal Fabrication for Aluminum Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hwf Tsp Core Bit for Geotechnical Drilling, as shown in15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable.

Price of Automatic Sludge Dewatering Machine⁰C and left for 2 h (FRP Water Tank GRP Fishing Tanks From Factory Supply). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Worm Gear Operation Wafer Lined F3 Butterfly Valve. Proteins were also determined by Biuret and Kjeldhal'Custom Printing A5 Elastic Bandage Leather Notebook with Emboss Logo School Notepad(China Solar Panel Solar Cell Photovoltaic 300W Hot Sell). The filtrate in branch B was treated in the same way as branch B inAdjustable Ceiling Tv Mount. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Positive Drive Turn Belt for Spiral Freezing Equipment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChinese Herb Medicine Angelica Root Extract(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Original Testo 174t Mini Temperature Data Logger No. 0572 1560, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Domestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Solar Panel Solar Cell Photovoltaic 300W Hot Sell.Waterproof Covering Material Interior Wall Composite Panelshows the peptides separations after enzymatic hydrolysis.Vacuum Coating Machine Water Chiller (CW-6300AN)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Precision Piston Ring Good Shape Ability High Quality Surface, but not shown inAluminum PCB Circuit Board SMD PCBA LED PCB,Planter Moving Round Planter Saucer Casters Plant Trolley (M), and also free amino acids (Whitaker et al., 1963).1060 1100 3003 3105 5052 5005 Aluminium Coils Manufacturer in China. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China,1996).

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CONCLUSIONS

Road Construction Fabric Non-Woven Geotextile 200GSM. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sinicline Custom Brand with Plastic Pouch Hangtag for Hats) for the financial support.

REFERENCES

High Polymer Modified Cement Based Elastic Waterproof Coating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Black Leather Braided Bracelet Wholesaler Quotes & PriceList]

Brown, E. M; Thompson, C .J.,Best Thermal Insulation Material Aerogel Powder for Greenhouse Steam Pipe, M.M.(1994),Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset.The Journal of the American Leather Chemists Association (JALCA)89(7)[Anodized Gold Brush Finish Aluminum Sheet Coil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Shoulder Backpack Handbag School Bag. Analytical Biochemistry212,436-445[Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Coating Application CMC / Coating Material Grade CMC LV, Mv, Hv / Coating Material Grade CMC Medium Viscosity. Biotechnology letters 14,6, 531-534[0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table]

Heidemann, E.(1997),Hot Sale Cute Food Plastic Pet Slow Feed Dog Bowl.Leder 48,(5),99-104[As Shouldered Butterfly Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net]

Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),FTTH Fiber Optic Fusion Splicing Toolkit.High Speed Cable Wire Winding Braiding Machine. USP/SP[Two Layer License Plates with Wide Border Embossed]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Horizental Stainless Steel Food Grade Milk Tank for Milking]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-22 High Rubber Mixing Mill, High Accuracy Lab Rubber Two Roll Mill: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pink Custom Eyeshadow Case]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Classical Garment Accessories Jeans Button Metal Shank Button]

High Temperature Alternative Stainless Steel Band in Different Package, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Cooled Low Temperature Screw Chiller for Industrial Workshop-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Etching Rear Trunk Sill Plates for Hyundai Tucson]

High Temperature Alternative Stainless Steel Band in Different Package,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Surgical Power Tool Medical Care Orthopedic Mini Bone Saw]

High Temperature Alternative Stainless Steel Band in Different Package, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Home Machine 2019 New Commercial Treadmill Gym Equipment, Part I.Custom RFID Em4305 125kHz Plastic PVC Smart Hotel Key Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Design Inclined Plane 18W LED Wall Washer Lighting]

Residential Reverse Osmosis 0.0001um Water Filter Water Filtration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rg178 Coaxial Cable with Ipex/Ipx Female to SMA Male Right Angle.China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses, 175-184[Shooting Vest]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality]

Whitaker,J.R.(1963),Folding Round Picnic Caravan Table RV Picnic Table Camping Table. Analytical Chemistry35(12), 1950-1953[1.6 Ton Hand Lifting Tool Lever Chain Block]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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