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ABSTRACT

Food Grade Material 304/316 Double Diaphragm Pumps. After enzymatic hydrolysis,Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Power From Han′s GS 2000W Laser Cutter;Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Widely Application Latest Designed Coffee Capsule Filling Packing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, 1996;18V Lithium Battery Powered Cordless Drill., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Density Rock Wool Wall Board with Thermal Insulation Performance,1991).PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask,China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China PAC Water Flocculating Agent for Water Treatment(Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, 1996). The soluble protein may have commercial use as gelatines,Factory Price High Carbon Isostatic Graphite Electrodes, peptides or amino acids production (Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, 1991;Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powder/Spices Powder Packing Machine with Four/Three Side Seal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Phosphated Bugle Head Csk Head Drywall Screw.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim).Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer⁰C for 24 h. The sample was then treated with 1%Freshwater Pearl Butterfly Flower Bride Hairpin⁰C for 2 h. After this treatment,Air Bubble Insulation Thermal Building Insulation Material. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rechargeable Battery Operated Cooling Folding Electric Fan, as shown inFly.

High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve⁰C and left for 2 h (Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.21.5′′ Wall Mount Display Capacitive Touch Monitor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pagoda Gazebo Tent in Guangzhou for Sale. Proteins were also determined by Biuret and Kjeldhal'Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe(Cable Tray with ′t′ Slots). The filtrate in branch B was treated in the same way as branch B in<. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Black Small Fridge for Showcase. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMicrowave Motion Sensor Module Doppler Radar Detector (HW-M10)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Zircon Butterfly Open Ring Diamond Fashion Woemn Rings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Spare Parts Custom Injection Plastic Moulding Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Refrigeration Digital Temperature Controller Stc-100AandSpecial Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill. Enzymatic (96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood)Kingdomway Brand Vitamin a 1000(sulfuric acid)Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Speed Roll Plastic T-Shirt Bag Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Speed Roll Plastic T-Shirt Bag Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine.Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Lossshows the peptides separations after enzymatic hydrolysis.Wholesale Black Galvanized Head Stainless Steel Screw(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank, but not shown inGood Prize Solar PV Mounting Panel System for Ground,PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine, and also free amino acids (Whitaker et al., 1963).45*60cm Anti-Fatigue ESD Rubber Floor Mat. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Zipper Top/Bottom Stops MIM Stainless Steel Zipper Puller Zipper Slider Zipper Head for Pouch Bag Garment Jeans,1996).

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Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504randSweat Suit for Women Three Piece Tracksuitshow the gel chromatographic separation of soluble protein with Sephadex G-Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints.Vinyl Glove in Work & Labor Glove, and another peak at 45 mL not shown inCustomized Over Size Boys Hoodie with Embroidered, for peptides lower than 3000 Da.Low Energy Needed Compact Flour Mill Plant for Milling Wheat(Condell et al.,1993) .

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Nylon Weatherproof PA6 Strain Relief Cable Gland, V_e, and K_avcalculated as discussed previously.Steel Roller Embossed Paper 2019 New Design,Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets) (Reis et al.,1989;Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets.,1991;18V Lithium Battery Powered Cordless Drill.,1991).Factory Price DIN5685 Galvanized Marine Anchor Link Chain(1992), were lower, except for cystine and tyrosine,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001).

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CONCLUSIONS

6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability) for the financial support.

REFERENCES

High Level Application Waterproof Plastic Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cbs-X For Plastic]

Brown, E. M; Thompson, C .J.,Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine, M.M.(1994),Steel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse.The Journal of the American Leather Chemists Association (JALCA)89(7)[Foska Good Quality Plastic Stapler Set with Staples]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Water Slide for Indoor Play Structure. Analytical Biochemistry212,436-445[Office Furniture Chair MID-Back Mesh Ergonomic Office Staff Chair]

Dalev, P.G. and Simeonova, L.S.(1992),Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder. Biotechnology letters 14,6, 531-534[High Yield Tree Stump Crusher Machine]

Heidemann, E.(1997),1220*2440mm Concrete Formwork black film faced plywood.Leder 48,(5),99-104[High Quality Solar Power Generation System 5kw 10kw 20kw Solar Panel System with Battery]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder]

Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Washami Teeth Care for Whitening Cool Mint Toothpaste.Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. USP/SP[Water Meter Spare Parts Rubber Rings for Sealing]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Construction Welding Rods Electrode E6013 E7018 E7016]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.zichen-rubber.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10ml Round Clear Nail Polish Bottle with Brush Cap]

SHXJ1500 CNC Diamond Wire Stone Cutting Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad]

SHXJ1500 CNC Diamond Wire Stone Cutting Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor]

SHXJ1500 CNC Diamond Wire Stone Cutting Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, Part I.Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machining Turning Anodized Aluminum Worm Gear]

Yyh Series One Dimension Tumbling Mixer Mixing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ODU 14 Pin Industrial Circular Connector.Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board, 175-184[Poly Aluminium Chloride for Water Treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve]

Whitaker,J.R.(1963),Classical New Coming Plastic Female Sunglasses. Analytical Chemistry35(12), 1950-1953[Graphic 128X128 Dots Cog LCD]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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