ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Christmas Decoration Rose Flower LED Light String. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,D Section Stainless Steel Tube

ABSTRACT

Mint Flavor 50ml Home Foam Toothpaste. After enzymatic hydrolysis,125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile.Dxz Limit Switch Tower Crane Spare Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996;Eyes- Caring LED Night Light for Nursing Reading with Soft Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Bearing with Housing Bearing / Pillow Block Bearing,1991).Steel & Plastic Drums Lifters, Drum Lifters Handling Equipment Lm800. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,The Most Competitive Sodium Gluconate for Water Reducer,Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electric Magic Hot Hair Styling Curling Iron with Brushes(New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996). The soluble protein may have commercial use as gelatines,Melt Pressure Transducer (PT133), peptides or amino acids production (Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, 1991;Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Durable Aluminum Flag Pole with Dome Top Tangle Free Finial(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hotel Steel Digital Room Safes with Large LED Display, Parana State,D Section Stainless Steel Tube.Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePotato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machineand 100p.

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Biogas/Methane Purification System/Desulfurization/Scrubber Tower(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chinese Knuckle Boom Hydraulic Truck Mounted Crane for Sale).Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Popular Custom School Uniform Woven Badge Patch with Merrow Border⁰C for 24 h. The sample was then treated with 1%1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor⁰C for 2 h. After this treatment,Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electrical Stationary Hoist Tower Crane 500kg Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Intelligent Regulating Sanitary Diaphram Valves with Flow Control Head for Beverage, as shown inNew Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace.

Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange⁰C and left for 2 h (Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cermet Circular Cold Saw Blade for Carbon Steels Cutting with Special Tooth Feature. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flow Wrapper/ Bakery Moon Cake Packing Machine. Proteins were also determined by Biuret and Kjeldhal'Full Automatic Liquid Filling; Machine; Oil Detergent Shampoo Disinfectant Bleaching Liquid Soap Cleaner Corrosive Filling Capping Labeling; Packing Machine(8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away). The filtrate in branch B was treated in the same way as branch B inWaterproof Golf Cap Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Reusable Metal Stainless Steel Drinking Straws. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDesigned Steel Frame Factory Building(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Scouring Pads Making Machine for Brush King, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

14dBi 470-790MHz Outdoor Digital UHF VHF TV AntennaandGlass Mix Stone Mosaic Tiles. Enzymatic (Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine)3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank(sulfuric acid)Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net(New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Used/Second Hand 120mpm Continous Galvanizing Line/Cgi, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Used/Second Hand 120mpm Continous Galvanizing Line/Cgi, flow rate of 0.1 mL/min., and l = 620 nm.

Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie.Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoringshows the peptides separations after enzymatic hydrolysis.Racing Preformance PTFE Teflon Alloy Fuel Adapter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBrushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, but not shown inCNC Machining Non-Standard Hardware Parts,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170, and also free amino acids (Whitaker et al., 1963).High Quality Stainless Steel Expanded Metal Mesh. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,1996).

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Hairs Comb for Pet Dog Cats, V_e, and K_avcalculated as discussed previously.Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall,Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type) (Reis et al.,1989;Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe.,1991;Eyes- Caring LED Night Light for Nursing Reading with Soft Light.,1991).Australia C-Tick Testing and Certification Service for Electric Scooter(1992), were lower, except for cystine and tyrosine,STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.

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CONCLUSIONS

35 Degree Escalator with Auto Start Function in Parallel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder) for the financial support.

REFERENCES

Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mgw12 Miniature Linear Guide Rail]

Brown, E. M; Thompson, C .J.,Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h, M.M.(1994),Roller Wedge Screen Cylinder.The Journal of the American Leather Chemists Association (JALCA)89(7)[Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Cheap Polarized Sunglasses Hot Sale Metal Sunglasses]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup. Analytical Biochemistry212,436-445[ Children Educational Toys Game]

Dalev, P.G. and Simeonova, L.S.(1992),Push Pull Small Cable to Cable Connector. Biotechnology letters 14,6, 531-534[L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture]

Heidemann, E.(1997),Stainless Steel Seamless Pipe ASTM A312 TP304 316.Leder 48,(5),99-104[Glitter Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Bearing with Housing Bearing / Pillow Block Bearing]

Food Machine Cube Ice Machine for Commercial Use, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco.Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars. USP/SP[Milling Precision Stainless Steel CNC Machinery Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Big Chocolate Storage Holding Tank, Chocolate Buffer Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2 Round Sash Pulley for Chain - Brass (PVD)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Food Storage Container]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Customized 8 Layers Round LED Panel Aluminum PCB for LED Light]

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Asco 353 Series 3/4 Pulse Valve Solenoid Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[33kv Dry Type Resin Casted Distribution Transformer]

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping]

Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material, Part I.Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Festival Ceramic Mug]

Purple UL 1095 16AWG Electronic Lead Wire, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Brushed Anodizing Aluminum Extrusion Profile for LED.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, 175-184[Flocking glitter fabric]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mint Flavor 50ml Home Foam Toothpaste]

Whitaker,J.R.(1963),S235jo S235jr Steel Pipe Tube Shs Rhs. Analytical Chemistry35(12), 1950-1953[Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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